Disease relevance of Akap1

• In this study we tested the hypothesis that ribosomal protein extracts (RSW) from glioma cells contain PKA regulatory (RII) and catalytic (C) subunits that, in combination with a protein kinase A anchoring protein (AKAP 95) and CSR-BPs participate in forming CSR-protein complexes that are responsible for mRNA stability regulation [1].

High impact information on Akap1

• These findings indicate that PKA anchored by AKAP121 transduces cAMP signals to the mitochondria, and it may play an important role in mitochondrial physiology [2].
• An AKAP121 derivative mutant that localizes on mitochondria but does not bind PKA down-regulates PKA signaling to the mitochondria and promotes apoptosis [2].
• Here we show that conditional expression of AKAP121 in PC12 cells selectively enhances cAMP.PKA signaling to mitochondria [2].
• Differentiated thyroid cells (TL5) accumulate AKAP121 upon incubation with TSH or a cAMP analog [3].
• AKAP121 is induced with similar kinetics when an unrelated, spermatocyte-derived cell line (GC-2) is incubated with 8-chlorophenylthio-cAMP [3].

Biological context of Akap1

• Identification and characterization of D-AKAP1 as a major adipocyte PKA and PP1 binding protein [4].

Anatomical context of Akap1

• AKAP121 binds and targets PKAIIalpha to the cytoplasmic surface of mitochondria [3].
• Immunohistochemistry has illustrated that NOS2 was intensely accumulated in Sertoli and germ cells in the epithelium during adjudin-induced germ cell loss, with a concomitant accumulation of intracellular cGMP and an induction of PRKG but not cAMP or protein kinase A (cAMP-dependent protein kinase, PRKA) [5].
• This protein was found to be enriched in the lipid droplet fraction of primary adipocytes and was identified as D-AKAP1 [4].
• Because the labeling patterns for RIalpha and D-AKAP 1 are similar in the muscle fibers and at the postsynaptic membrane, it may be that this AKAP anchors RIalpha in these regions [6].
• RESULTS: Immunohistochemistry and confocal microscopy were used here to determine that D-AKAP1 colocalizes with RIalpha at the postsynaptic membrane of the vertebrate neuromuscular junction (NMJ) and the adjacent muscle, but not in the presynaptic region [6].

Associations of Akap1 with chemical compounds

• AKAP121 mRNA accumulated in the presence of cycloheximide, suggesting that transcription of the anchor protein gene is directly controlled by cAMP and PKA [3].
• Thus, AKAP121 concentration may be controlled by hormones that activate adenylate cyclase [3].

Analytical, diagnostic and therapeutic context of Akap1

• Levels of total and newly synthesized AKAP121 mRNA also increased after treatment [3].

References