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Gene Review

repA  -  plasmid replication protein

Escherichia coli

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Disease relevance of repA

  • It was observed that when the repA and repB genes were expressed in Escherichia coli, a strong ori binding activity was generated in the host cells [1].
  • A derivative of pKL1, pKL1Km, in which the transposon was restricted to a small 350-bp region, was stably maintained in Shigella, Salmonella, Serratia, and Citrobacter species and its replication was polA independent. pKL1 encoded only a single protein, RepA (Mr 17960), which specifically bound to pKL1 DNA [2].
  • RepA protein of the bacteriophage N15 exhibits activity of DNA helicase [3].

High impact information on repA


Biological context of repA

  • The cop region also harbors a region of plasmid incompatibility, inc, encompassing a stem-loop structure, the repA promoter, Prep, as well as two distinct RepA binding sites, BD-1 and BD-2 [6].
  • Both RepA binding sites were required to maintain incompatibility and effective pKL1 replication [6].
  • RepA protected a 68-bp region of the origin of replication of pMU720 [7].
  • The nucleotide sequence analysis revealed the presence of an open reading frame encoding a putative 317 amino acids protein homologous to replication proteins RepA [8].
  • Residues K42, E76, D77, D139 and H178, proposed to play key roles in catalyzing the hydrolysis of NTPs, are essential for RepA activity [9].

Analytical, diagnostic and therapeutic context of repA


  1. Origin binding activity of the Mycobacterial plasmid pAL5000 replication protein RepB is stimulated through interactions with host factors and coupled expression of repA. Basu, A., Chawla-Sarkar, M., Chakrabarti, S., Das Gupta, S.K. J. Bacteriol. (2002) [Pubmed]
  2. Small cryptic plasmids of multiplasmid, clinical Escherichia coli. Burian, J., Guller, L., Macor, M., Kay, W.W. Plasmid (1997) [Pubmed]
  3. RepA protein of the bacteriophage N15 exhibits activity of DNA helicase. Mardanov, A.V., Strakhova, T.S., Ravin, N.V. Dokl. Biochem. Biophys. (2004) [Pubmed]
  4. The RepA protein of plasmid pSC101 controls Escherichia coli cell division through the SOS response. Ingmer, H., Miller, C., Cohen, S.N. Mol. Microbiol. (2001) [Pubmed]
  5. Translational coupling to an upstream gene promotes folding of the mycobacterial plasmid pAL5000 replication protein RepB and thereby its origin binding activity. Basu, A., Chatterjee, S., Das Gupta, S.K. J. Bacteriol. (2004) [Pubmed]
  6. Replication control of a small cryptic plasmid of Escherichia coli. Burian, J., Stuchlík, S., Kay, W.W. J. Mol. Biol. (1999) [Pubmed]
  7. Interaction of the initiator protein of an IncB plasmid with its origin of DNA replication. Betteridge, T., Yang, J., Pittard, A.J., Praszkier, J. J. Bacteriol. (2003) [Pubmed]
  8. Characterization of pC7 from Lactobacillus paraplantarum C7 derived from Kimchi and development of lactic acid bacteria--Escherichia coli shuttle vector. Park, W.J., Lee, K.H., Lee, J.M., Lee, H.J., Kim, J.H., Lee, J.H., Chang, H.C., Chung, D.K. Plasmid (2004) [Pubmed]
  9. Hexameric RSF1010 helicase RepA: the structural and functional importance of single amino acid residues. Ziegelin, G., Niedenzu, T., Lurz, R., Saenger, W., Lanka, E. Nucleic Acids Res. (2003) [Pubmed]
  10. Hexamerization of RepA from the Escherichia coli plasmid pKL1. Burian, J., Ausió, J., Phipps, B., Moore, S., Dougan, D., Kay, W. Biochemistry (2003) [Pubmed]
  11. Comparative analyses of two cryptic plasmids from Haemophilus somnus (Histophilus somni). Siddaramappa, S., Duncan, A.J., Brettin, T., Inzana, T.J. Plasmid (2006) [Pubmed]
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