High impact information on Itgb2l

• In addition, intracellular Pactolus translocates to the neutrophil surface following induction of apoptosis [1].
• The increased expression of the membrane form of Pactolus by granulocytes of C57BL/6 mice suggests a compensatory adhesion function that is reduced in cells from the low producing strains [2].
• One gene was identified that was preferentially expressed in the SCF-derived cells and encodes a novel murine integrin beta subunit-like molecule, dubbed Pactolus-1 (Pactolus) [3].
• Identification of pactolus, an integrin beta subunit-like cell-surface protein preferentially expressed by cells of the bone marrow [3].
• Two distinct forms of Pactolus mRNA were detected which, via alternative splicing, are predicted to encode a membrane-bound form and truncated version of the protein [3].

Biological context of Itgb2l

• The murine Pactolus gene is comprised of 16 exons that encompass 24 kb of the genome [4].
• The Pactolus gene was mapped to a terminal region of murine Chromosome (Chr) 16, distinct from the previously mapped site of the beta2 integrin gene on murine Chr 10 [4].
• Transfection of Pactolus constructs into mouse macrophages, which do not express Pactolus, defined a negative control element within the first 100 base pairs [5].
• The presence of this negative regulatory site, distinct from the PU.1-binding site, was confirmed by EMSA oligonucleotide competition and gene reporter assays of Pactolus/CD18 chimeric constructs [5].
• The analysis of mice genetically deficient in Pactolus, however, did not define any lesion in neutrophil migration, adhesion or phagocytosis [6].

Anatomical context of Itgb2l

• These data suggest that Pactolus does not function as a neutrophil phagocytic or adhesion receptor, but may instead serve as a sugar-bearing ligand for lectin recognition by other cells [1].

Regulatory relationships of Itgb2l

• Sequence analysis of the negative control region in the Pactolus promoter suggested potential control by Snail and/or Smad families of transcription regulators [5].
• The numbers and expression patterns of these resident CXCL13-expressing cells do not vary in na??ve animals of wild type or Pactolus-deficient origin [6].

Other interactions of Itgb2l

• Transcriptional control of Pactolus: evidence of a negative control region and comparison with its evolutionary paralogue, CD18 (beta2 integrin) [5].
• This screen identified a single transcript, CXCL13, that was elevated in cells from a peritoneal lavage of the wild type animal compared to the Pactolus-deficient animal [6].
• The resident CXCL13-expressing cells (which do not express Pactolus) are functionally distinct from the macrophages recruited into the peritoneal cavity following the inflammatory stimulation since the recruited macrophages do not express detectable levels of the chemokine [6].

Analytical, diagnostic and therapeutic context of Itgb2l

• Immunoprecipitation of Pactolus revealed a cell-surface protein with an apparent molecular mass of about 95 kDa [3].
• This intracellular form of Pactolus can be released to the cell surface following inflammatory activation or ligation of Pactolus on the cell surface [1].

References