Disease relevance of Ms6hm

• The hypervariable mouse minisatellite locus Ms6hm was found to be highly unstable, specifically among sarcomas with the amplified c-myc gene [1].
• 6. A 7-kb allele of Ms6-hm was cloned from a C57BL/6J mouse and collapsed to a 400-bp plasmid insert on propagation in Escherichia coli due to loss of the majority of minisatellite repeat units [2].
• Effect of SCID mutation on the occurrence of mouse Pc-1 (Ms6-hm) germline mutations [3].

High impact information on Ms6hm

• Length change mutation at the Ms6hm hypervariable mouse minisatellite locus was analyzed in C57BL/6N x C3H/HeN F(1) mice and the F(1) of the reciprocal cross born to irradiated male parents [4].
• The mouse Ms6-hm hypervariable microsatellite forms a hairpin and two unusual tetraplexes [5].
• Somatic mutation at the Ms6-hm minisatellite locus was nearly seven times higher (27%, 4/15) than the spontaneous germline mutation rate (4%) [6].
• The characteristics of DNA sequences flanking the mouse minisatellite Ms6-hm have been investigated [7].
• The mouse minisatellite locus Ms6-hm has a germline mutation rate of 2.5% per gamete and is therefore one of the most unstable loci yet identified in the mouse genome [8].

Biological context of Ms6hm

• Paternal 60Co gamma-irradiation was tested for the induction of germline mutation at the mouse hypervariable minisatellite locus, Ms6hm [9].
• Spontaneous mutation at the hypervariable mouse minisatellite locus Ms6-hm: flanking DNA sequence and analysis of germline and early somatic mutation events [8].
• Under low-stringency hybridization conditions the collapsed subclone of Ms6-hm cross-hybridizes to other unstable loci in the mouse genome to generate a novel and highly individual specific mouse DNA fingerprint [2].
• Linkage analysis localized Ms6-hm near the brown coat color gene (b) on chromosome 4 [2].
• Sequence analysis revealed that Ms6-hm has evolved by amplification within a member of the MT (mouse transcript) family of interspersed repetitive elements [2].

Anatomical context of Ms6hm

• The paternal mutation frequencies per gamete of the Ms6hm locus were 8.3, 13, 28 and 15% for the C3H/HeN control, exposed spermatozoa, spermatids and spermatogonia stages, respectively [9].
• These somatic mutation events at Ms6-hm must therefore occur very early in development, preceding the allocation of somatic lineages, and the same pool of primitive ectoderm cells must contribute equally to all somatic tissues [2].
• We used single-molecule-polymerase chain reaction (SM-PCR) to assess the relative mutant frequency after 4-week chemical treatments at the Ms6-hm ESTR sequence of cultured C3H/10T1/2 cells (a mouse embryonic cell line) [10].

Analytical, diagnostic and therapeutic context of Ms6hm

• DNA samples of F1 offsprings were analysed by Southern blotting for the repeat length mutation at the Ms6hm locus [11].
• We now show that repeat DNA instability at the mouse ESTR locus Ms6-hm can also be monitored by single molecule PCR analysis of genomic DNA [12].

References