Disease relevance of Morf4l1

• We determined by chromatin immunoprecipitation that MRG15 was recruited to the alpha-globin promoter during dimethyl sulfoxide-induced mouse erythroleukemia cell differentiation [1].

High impact information on Morf4l1

• In contrast, MRGX, which evolved later, is expressed only in vertebrates, suggesting that the lack of phenotype of MrgX-deficient mice is secondary to a compensatory effect by the evolutionarily conserved MRG15 protein but not vice versa [2].
• MRG15 regulates embryonic development and cell proliferation [1].
• Cell proliferation defects are also observed in Mrg15-/- mouse embryonic fibroblasts [1].
• These findings demonstrate that MRG15 has an essential role in embryonic development via chromatin remodeling and transcriptional regulation [1].
• Mrg15-/- embryos appeared pale, and microarray analysis revealed that alpha-globin gene expression was decreased in null versus wild-type embryos [1].

Biological context of Morf4l1

• Characterization of the expression pattern of mouse MrgX demonstrated it was ubiquitously expressed in all tissues of adult mice and also during embryogenesis and overlapped with its homolog Mrg15 [2].
• MRG15 associates with at least two nucleoprotein complexes that include histone acetyltransferases and/or histone deacetylases, suggesting it is involved in chromatin remodeling [1].
• Immunohistochemical analysis indicates that apoptosis in Mrg15-/- embryos is not increased compared with wild-type littermates [1].
• The mouse MRG15 gene is composed of twelve exons and spans over 24 kb DNA [3].
• Pf1 has independent binding sites for MRG15 and mSin3A [4].

Anatomical context of Morf4l1

• MRGX and MRG15 proteins localize predominantly to the chromatin fraction in the nucleus, although a small amount of both proteins localized to the nuclear matrix [2].
• Mouse MRG15 is expressed ubiquitously in adult tissues and at various embryonic stages, and expression in adult testis is higher than in other tissues [3].
• Immunohistochemistry with anti-MRG15 antibodies revealed that MRG15 was localized in dendrites as well as in the nuclei of Purkinje cells [5].

Other interactions of Morf4l1

• To study the role of MRG15 in vivo, we generated knockout mice and determined that the phenotype is embryonic lethal, with embryos and the few stillborn pups exhibiting developmental delay [1].

References