High impact information on Ube1y1

• Here we show that introduction of Ube1y1 and Dby as transgenes into Sxrb-deletion mice fails to overcome the spermatogenic block [1].
• Marsupial Y chromosome encodes a homologue of the mouse Y-linked candidate spermatogenesis gene Ube1y [2].
• We report here the identification of a functional marsupial Y-linked homologue of the murine Ube1y gene establishing that Metatherian and Eutherian Y chromosomes diverged from a common ancestor [2].
• We also detected two copies of the gene on the short arm of the mouse Y chromosome (A1s9Y-1 and A1s9Y-2) [3].
• Therefore A1s9Y-1 is a candidate for the spermatogenesis gene, Spy, which maps to this region [3].

Biological context of Ube1y1

• Ube1 is located at band A2 of the mouse X Chromosome (Chr) and Ube2 on the short arm of the Y Chr [4].
• Ube1y is a Y-linked gene transcribed in the testis, which maps to a region of the mouse Y required for normal spermatogonial proliferation [5].

Anatomical context of Ube1y1

• The functional A1s9Y gene (A1s9Y-1) is expressed in testis and is lost in the deletion mutant Sxrb [3].
• At various times after injection, APC numbers in the draining (axillary and brachial) lymph nodes were assessed using a Ube1y gene-specific PCR assay [6].
• The presence of Ube1x transcripts in A spermatogonia raises the question as to why Ube1y transcripts are required [5].
• Analysis of XXSxr(a) testes indicates that the bulk of the Ube1y transcription is in germ cells [5].
• Transcriptional analysis of the candidate spermatogenesis gene Ube1y and of the closely related Ube1x shows that they are coexpressed in spermatogonia and spermatids but are repressed in pachytene spermatocytes [5].

Other interactions of Ube1y1

• The ubiquitin-activating enzyme E1 homologous genes on the mouse Y chromosome (Ube1y) represent one functional gene and six partial pseudogenes [7].

References