Disease relevance of Csde1

• All proviruses were integrated in the same transcriptional orientation as unr and N-ras genes [1].
• Transient expression of Unr in unr(-/-) cells efficiently restored the HRV and poliovirus IRES activities [2].
• Thus, altogether our findings indicate that overexpression of N-ras plays a role in development of murine leukemia virus-induced B-cell lymphomas, leaving the expression of the tightly linked unr gene unaltered [1].
• In this report, we rationally designed and synthesized three antisense and one sense hybrid PNA (peptide nucleic acid) to the unr mRNA that is highly overexpressed in a breast cancer cell line (MCF-7) [3].

High impact information on Csde1

• These assays also confirmed the short nucleotide distance interposed between the N-ras transcription unit and the previously described upstream unr gene [4].
• We also showed that ras genes can transactivate the unr promoter activity and that the 90 bp fragment responded to this transactivation [5].
• Neither the primary protein structure nor the nucleic acid sequence of unr is homologous to any other known gene, including N-ras [6].
• An open reading frame, potentially encoding a 798 amino acid protein, is contained within the unr cDNA [6].
• The activity of picornaviral IRES elements was analyzed in unr(+/+), unr(+/-), and unr(-/-) cell lines [2].

Biological context of Csde1

• Different developmental pattern of N-ras and unr gene expression in mouse gametogenic and somatic tissues [7].
• Organization of the unr/N-ras locus: characterization of the promoter region of the human unr gene [5].
• These two pseudogenes, together with the wildtype N-ras gene and a small 3' part of the unr gene, were eventually cloned and their genomic organization and nucleotide sequences determined [8].
• First, antisense binding sites on the unr mRNA were mapped by an reverse transcriptase random oligonucleotide library (RT-ROL) method that we have improved, and by a serial analysis of antisense binding sites (SAABS) method that we have developed which is similar to another recently described method [9].

Anatomical context of Csde1

• However, the expression of unr and its alternative transcripts is developmentally and differentially regulated in small intestine, heart and testis [7].
• To investigate this possibility, we have deleted the unr promoter by homologous recombination in murine embryonic stem cells [10].
• To study the nucleic acid-binding properties of unr, a protein with multiple repeats of a nucleic acid-binding motif, we expressed the long splicing isoform in a eukaryotic cell line and purified it in native form [11].

Regulatory relationships of Csde1

• Specifically, transcription of unr could negatively regulate that of N-ras by transcriptional interference [10].

Other interactions of Csde1

• Characterization of unr; a gene closely linked to N-ras [6].

Analytical, diagnostic and therapeutic context of Csde1

• PCR amplification and sequence analyses of DNA flanking integrated proviruses revealed proviral insertion into the N-ras/unr (upstream of N-ras) locus in 2 out of 13 B-cell lymphomas, both of which appeared clonal by Southern blotting analysis [1].

References