Disease relevance of Klk7

• Transgenic mice expressing human stratum corneum chymotryptic enzyme in suprabasal epidermal keratinocytes were found to develop pathologic skin changes with increased epidermal thickness, hyperkeratosis, dermal inflammation, and severe pruritus [1].
• The results suggest that stratum corneum chymotryptic enzyme may be involved in the pathogenesis of inflammatory skin diseases, and that stratum corneum chymotryptic enzyme and related enzymes should be evaluated as potential targets for new therapies [1].
• We have previously reported on increased expression of the stratum corneum chymotryptic enzyme in psoriasis [1].
• Here is reported an increased epidermal expression of stratum corneum chymotryptic enzyme also found in chronic lesions of atopic dermatitis [1].
• Epidermal overexpression of stratum corneum chymotryptic enzyme in mice: a model for chronic itchy dermatitis [1].

High impact information on Klk7

• LEKTI deficiency causes abnormal desmosome cleavage in the upper granular layer through degradation of desmoglein 1 due to stratum corneum tryptic enzyme and stratum corneum chymotryptic enzyme-like hyperactivity [2].
• The serine protease stratum corneum chymotryptic enzyme is preferentially expressed in cornifying epithelia [1].
• It had an open-reading frame encoding a protein comprising 249 amino acids with 82% amino acid sequence homology to human SCCE including the conserved sequences of the catalytic traid of mammalian serine proteases [3].
• Human stratum corneum chymotryptic enzyme (SCCE) may play a central part in epidermal homeostasis [3].
• As in human SCCE the P2 position of the propeptide was occupied by an acidic amino acid residue, and the position corresponding to the suggested bottom of the primary substrate specificity pouch occupied by an asparagine residue [3].

Biological context of Klk7

• The murine protein was deduced to have a 21 amino acid signal peptide and a four amino acid propeptide ending with a tryptic cleavage site, followed by a sequence motif identical to the N-terminal amino acid sequence of native active human SCCE [3].

Anatomical context of Klk7

• A correlation therefore seems to exist between the presence of SCCE in high suprabasal cells and the ability of the epithelium to form an orthokeratotic cornified layer [4].

Analytical, diagnostic and therapeutic context of Klk7

• 2. Recombinant SCCE induced a significant decrease in the immunoblotting of both beta-GlcCer'ase or aSMase compared with control experiments performed in the absence of the active SCCE [5].
• Molecular cloning and tissue expression of the murine analog to human stratum corneum chymotryptic enzyme [3].
• Two mouse monoclonal antibodies (MAb) were raised, TE4b and TE9b, that reacted specifically with SCCE in immunoprecipitation, immunoblotting, and gel-exclusion chromatography [4].

References