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Gene Review

cdc10  -  MBF transcription factor complex subunit...

Schizosaccharomyces pombe 972h-

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High impact information on cdc10

  • Mitosis and cytokinesis occur in the presence of hydroxyurea or in the double mutant cdc10-cut5 (the cdc10 mutation alone blocks progression from G1 to S) [1].
  • Activity of Start genes cdc2 and cdc10 is necessary and p34cdc2 kinase is active in re-replicating cells [2].
  • Appropriately timed expression of this N-terminal fragment of Cdc13 overcame the G1 arrest seen in cdc10 mutant strains, suggesting that proteins required for the initiation of S phase are subject to destruction by the same proteolytic machinery as cyclin [3].
  • We have recently isolated rep2+ as a multicopy suppressor of a temperature-sensitive cdc10 mutant which encodes a zinc finger protein [4].
  • Ectopic expression of cdt1 can complement a temperature sensitive mutation of cdc10 at semipermissive temperature [5].

Biological context of cdc10

  • Study of RNA extracted from cells both entering stationary phase and undergoing synchronous cell divisions suggests that commitment to the cell cycle is not controlled by regulation of cdc10 transcript level [6].
  • The putative cdc10 gene product shows no significant primary structure similarity with products of other fission and budding yeast cell cycle genes, or with other protein sequences in several databases [6].
  • Overexpression of the cdc10-binding domain of p72res1 leads to a G1 arrest with a cdc phenotype and a decrease on MBF activity [7].
  • We have used an immunoprecipitation-PCR cycle to isolate physically genomic DNA sequences that are bound by the fission yeast cdc10 gene product in an attempt to identify novel target genes [5].
  • An essential gene, cdt1, has been isolated whose expression is cell cycle regulated in a cdc10 dependent manner [5].

Anatomical context of cdc10

  • In cdc10 mutant cells, the SPBs seem not only to be duplicated but also to undergo partial maturation, including invagination of the nuclear envelope underneath the SPB [8].
  • The relationship between actin and cell wall deposition was further confirmed in three temperature-sensitive cell division cycle (cdc) mutants; cdc10, cdc11 and cdc13 [9].

Regulatory relationships of cdc10


Other interactions of cdc10


Analytical, diagnostic and therapeutic context of cdc10

  • Examination of the regulation of MCB motif-containing genes through the perturbation of DNA synthesis control/MCB-binding factor (DSC/MBF)-mediated transcription in arrested synchronous cdc10 mutant cell cultures revealed a subset of functional targets of the DSC/MBF transcription factor complex, as well as certain gene promoter requirements [13].
  • Here we have studied the cdc10 mutant by flow cytometry using different DNA-binding fluorochromes and found no evidence that the increased fluorescence signal was caused by mitochondrial DNA synthesis [14].


  1. Fission yeast cut5+, required for S phase onset and M phase restraint, is identical to the radiation-damage repair gene rad4+. Saka, Y., Yanagida, M. Cell (1993) [Pubmed]
  2. A single fission yeast mitotic cyclin B p34cdc2 kinase promotes both S-phase and mitosis in the absence of G1 cyclins. Fisher, D.L., Nurse, P. EMBO J. (1996) [Pubmed]
  3. The role of proteolysis in cell cycle progression in Schizosaccharomyces pombe. Yamano, H., Gannon, J., Hunt, T. EMBO J. (1996) [Pubmed]
  4. Fission yeast Rep2 is a putative transcriptional activator subunit for the cell cycle 'start' function of Res2-Cdc10. Nakashima, N., Tanaka, K., Sturm, S., Okayama, H. EMBO J. (1995) [Pubmed]
  5. cdt1 is an essential target of the Cdc10/Sct1 transcription factor: requirement for DNA replication and inhibition of mitosis. Hofmann, J.F., Beach, D. EMBO J. (1994) [Pubmed]
  6. Cloning, sequencing and transcriptional control of the Schizosaccharomyces pombe cdc10 'start' gene. Aves, S.J., Durkacz, B.W., Carr, A., Nurse, P. EMBO J. (1985) [Pubmed]
  7. The Schizosaccharomyces pombe MBF complex requires heterodimerization for entry into S phase. Ayté, J., Leis, J.F., Herrera, A., Tang, E., Yang, H., DeCaprio, J.A. Mol. Cell. Biol. (1995) [Pubmed]
  8. Spindle pole body duplication in fission yeast occurs at the G1/S boundary but maturation is blocked until exit from S by an event downstream of cdc10+. Uzawa, S., Li, F., Jin, Y., McDonald, K.L., Braunfeld, M.B., Agard, D.A., Cande, W.Z. Mol. Biol. Cell (2004) [Pubmed]
  9. Growth polarity and cytokinesis in fission yeast: the role of the cytoskeleton. Marks, J., Hagan, I.M., Hyams, J.S. J. Cell Sci. Suppl. (1986) [Pubmed]
  10. A new cdc gene required for S phase entry of Schizosaccharomyces pombe encodes a protein similar to the cdc 10+ and SWI4 gene products. Tanaka, K., Okazaki, K., Okazaki, N., Ueda, T., Sugiyama, A., Nojima, H., Okayama, H. EMBO J. (1992) [Pubmed]
  11. Control over the onset of DNA synthesis in fission yeast. Simanis, V., Hayles, J., Nurse, P. Philos. Trans. R. Soc. Lond., B, Biol. Sci. (1987) [Pubmed]
  12. Cell division cycle mutants altered in DNA replication and mitosis in the fission yeast Schizosaccharomyces pombe. Nasmyth, K., Nurse, P. Mol. Gen. Genet. (1981) [Pubmed]
  13. Identification of cell cycle-regulated genes in fission yeast. Peng, X., Karuturi, R.K., Miller, L.D., Lin, K., Jia, Y., Kondu, P., Wang, L., Wong, L.S., Liu, E.T., Balasubramanian, M.K., Liu, J. Mol. Biol. Cell (2005) [Pubmed]
  14. Measurement of nuclear DNA content in fission yeast by flow cytometry. Carlson, C.R., Grallert, B., Bernander, R., Stokke, T., Boye, E. Yeast (1997) [Pubmed]
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