High impact information on Map3k4

• The forming acini expressed BRCA1, GADD45beta, MEKK4, and the JNK activating complex GADD 45beta-MEKK4 in a glucocorticoid-dependent fashion [1].
• Dominant negative forms of MEKK1 and MEKK4 were expressed stably in the clones harboring Q226L Galpha13 [2].
• Expression of dominant negative versions of either MEKK1 or MEKK4 effectively blocks both the activation of Jun N-terminal kinase as well as the formation of primitive endoderm [2].
• Neither t-specific nor wild-type D17Rp17 alleles are present in chromosomes carrying either the T Orleans (TtOrl) or the T hairpin tail (Thp) deletions [3].
• A study of several t haplotypes resulted in the identification of t-specific alleles of D17Rp17 that map to the proximal half of the t complex [3].

Anatomical context of Map3k4

• Not only anti-CD34 antibodies (HPCA-1) but also MTK1 could be shown to concentrate bone marrow CFU-GM and burst forming unit erythroid (BFU-E) effectively [4].
• MTK1 partially inhibited the stem cell factor (SCF) induced proliferation of M-MOK cells, whereas, MTK2 was without effect [4].

Other interactions of Map3k4

• The map distances have been determined for the DNA loci in the T-H-2 interval and the Hst-1 gene was mapped in close vicinity to the D17Rp17 locus [5].
• We describe here a 1.2-Mb yeast artificial chromosome (YAC) contig within the region of mouse chromosome 17 between Brachyury (T) and D17Rp17e, and spanning the quaking (qk) region [6].
• Detailed physical and genetic mapping in the region of plasminogen, D17Rp17e, and quaking [7].
• The genetic distance to D17Rp17e from D17Leh511 is also 0.15 cM; the physical distance of less than 360 kb (minimum 200 kb) is consistent with an approximation of 2 Mbp per cM [7].

Analytical, diagnostic and therapeutic context of Map3k4

• Both allowed visualization of the 140 kDa c-kit protein by Western blot analysis, but MTK1 detected only positive band with non-reducing conditions for sodium dodecyl sulfate-polyacrylamide gel electrophoresis [4].

References