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Gene Review

Hyou1  -  hypoxia up-regulated 1

Mus musculus

Synonyms: 140 kDa, 140 kDa Ca(2+)-binding protein, AI415631, CBP-140, Cab140, ...
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Disease relevance of Hyou1

  • Autoantibodies in the serum of patients suffering the blistering skin disease pemphigus vulgaris recognize a 140-kDa glycoprotein (GP) present in enriched fractions of bovine tongue epidermal desmosomes [1].
  • When lymphoma 140-kDa polypeptide is extracted from permeabilized cells using 25 mM MgCl2, capping is inhibited [2].
  • Hence, LCMV attachment to rodent fibroblastic cell lines is mediated by a glycoprotein(s) with a molecular mass of 120 to 140 kDa, with complex N-linked sugars that are not involved in virus binding [3].
  • Binding was specific, since unlabeled LCMV, but not the unrelated enveloped virus herpes simplex virus type 1, competed with 125I-labeled LCMV for binding to the 120- to 140-kDa band [3].
  • A glycoprotein of apparent molecular mass of 140 kDa, similar to that found in human B lymphocytes, was immunoprecipitated with anti-CD21 mAb and proved to be functional since both C3d and EBV bound efficiently and specifically to mouse cells expressing EBVR/CR2 [4].

Psychiatry related information on Hyou1


High impact information on Hyou1


Biological context of Hyou1


Anatomical context of Hyou1

  • Localization of CBP-140 in endoplasmic reticulum was shown by indirect immunofluorescence staining and also by subcellular fractionation [8].
  • Cultured Purkinje cells from Tg ORP150 mice displayed resistance to both hypoxia- and AMPA-induced stress [9].
  • Immunogold ultrastructural localization reveals that the 140-kDa antigen is localized not only along the intercellular area of the desmosome but also is found along the whole epidermal cell surface [1].
  • The products of these mutated c-erbB genes were about 30 kilodalton (kDa) smaller than the normal 170-kDa EGF receptor, and the tumor cell membrane fractions containing the 140-kDa abnormal EGF receptor showed a significant elevation of tyrosine kinase activity without its ligand [12].
  • Overexpression of axl cDNA in NIH 3T3 cells induces neoplastic transformation with the concomitant appearance of a 140-kDa axl tyrosine-phosphorylated protein [13].

Associations of Hyou1 with chemical compounds

  • The transcript has a 1 kb 5' untranslated region, and the first methionine initiation codon occurs in exon 51, predicting a protein of 140 kDa [7].
  • PLC gamma 2 appears as a 140-kDa protein distributed between particulate and soluble fractions which exhibits characteristic selectivity for phosphatidylinositol 4,5-bisphosphate and is sensitive to powerful activation by Ca2+ [14].
  • The final material consisted of a 140-kDa tyrosine and serine phosphorylated protein that was greater than 98% pure as assessed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of either [35S]methionine-labeled, silver-stained, or radioiodinated preparations [15].
  • The alpha(6) integrin is a 140-kDa (nonreduced) laminin receptor [16].
  • Expression of pp140c-trk in 3T3 fibroblasts (3T3-c-trk), as evidenced by cross-linking of 125I-NGF to the 140-kDa protein, permits the NGF-dependent activation of raf-1 kinase, detected in the immunoprecipitate kinase assay, anti-raf immunoblot shift on gel electrophoresis, and incorporation of [32P]orthophosphate into the raf-1 protein [17].

Physical interactions of Hyou1

  • An important step in this interaction involves the binding of a 140-kDa membrane protein that binds to fibronectin [18].

Regulatory relationships of Hyou1


Other interactions of Hyou1

  • Several AKAPs have been identified in oocytes including one at 140 kDa that we now identify as a product of the Akap1 gene [23].
  • Covalent affinity cross-linking of 125I-KGF to its receptor on Balb/MK cells revealed two species of 115 and 140 kDa [24].
  • Affinity labeling of TGF-beta 1 or TGF-beta 2 in Day 4 blastocysts revealed three classes of binding proteins with approximate molecular sizes of 65 kDa (type I), 90 kDa (type II), and greater than 250 kDa (type III), in addition to a doublet of 130 and 140 kDa proteins [25].
  • On immunoblotting SDS-PAGE-separated proteins from dense-grown cell monolayers, "pan-cadherin" antibodies have reacted with a band at approximately 140 kDa, identified as N-cadherin by peptide fingerprinting of the immunoprecipitated protein, which for reasons not yet clear is modified or masked in immunolocalization experiments [26].
  • Affinity cross-linking of [125I]IGF-I to microsomal membranes, under reducing conditions, revealed a binding moiety with an apparent molecular mass of 130 kDa in permissive cells and 140 kDa in inducible cells, which corresponded to the alpha subunit of the IGF-I receptor [27].

Analytical, diagnostic and therapeutic context of Hyou1

  • Immunofluorescence observations of cryostat sections of bovine tongue epidermis reveal that affinity-purified rabbit antibodies to the 140-kDa GP generate a punctate intercellular stain that is similar to that generated by antibodies directed against a desmosome plaque component (desmoplakin) [1].
  • Western blot analyses of mouse kidney revealed that two major bands (80 and 140 kDa) were detected with antibody M6749, but not with anti-HNK-1 antibody [28].
  • Rek protein was identified by immunoprecipitation and immunoblotting as a 140-kDa protein expressed in the chick retina at embryonic days 6-13, which corresponded to the major period of neuronal and glial differentiation [29].
  • Following incubation of intact cells with [adenylate-32P]NAD and analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), a 97-kDa [32P]ADP-ribosylated protein was observed under reducing conditions and a 140-kDa complex under nonreducing conditions [30].
  • By affinity chromatography of human ECV304 cell extracts on a MBP-p130Cas column followed by mass spectrometry matrix-assisted laser desorption ionization/time of flight analysis, we identified p140Cap as a protein migrating at 140 kDa [31].


  1. A cell surface desmosome-associated component: identification of tissue-specific cell adhesion molecule. Jones, J.C., Yokoo, K.M., Goldman, R.D. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
  2. The role of caldesmon in the regulation of receptor capping in mouse T-lymphoma cell. Walker, G., Kerrick, W.G., Bourguignon, L.Y. J. Biol. Chem. (1989) [Pubmed]
  3. Characterization of lymphocytic choriomeningitis virus-binding protein(s): a candidate cellular receptor for the virus. Borrow, P., Oldstone, M.B. J. Virol. (1992) [Pubmed]
  4. Stable expression and function of EBV/C3d receptor following genomic transfection into murine fibroblast L cells. Cantaloube, J.F., Piechaczyk, M., Calender, A., Lenoir, G., Minty, A., Carrière, D., Fischer, E., Poncelet, P. Eur. J. Immunol. (1990) [Pubmed]
  5. Existence of a putative specific postsynaptic density protein produced during Purkinje cell spine maturation. Miranda-Contreras, L., Palacios-Prü, E.L. Int. J. Dev. Neurosci. (1995) [Pubmed]
  6. The stimulatory action of tolbutamide on Ca2+-dependent exocytosis in pancreatic beta cells is mediated by a 65-kDa mdr-like P-glycoprotein. Barg, S., Renström, E., Berggren, P.O., Bertorello, A., Bokvist, K., Braun, M., Eliasson, L., Holmes, W.E., Köhler, M., Rorsman, P., Thévenod, F. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  7. Dp140: a novel 140 kDa CNS transcript from the dystrophin locus. Lidov, H.G., Selig, S., Kunkel, L.M. Hum. Mol. Genet. (1995) [Pubmed]
  8. CBP-140, a novel endoplasmic reticulum resident Ca(2+)-binding protein with a carboxy-terminal NDEL sequence showed partial homology with 70-kDa heat shock protein (hsp70). Naved, A.F., Ozawa, M., Yu, S., Miyauchi, T., Muramatsu, H., Muramatsu, T. Cell Struct. Funct. (1995) [Pubmed]
  9. ORP150/HSP12A regulates Purkinje cell survival: a role for endoplasmic reticulum stress in cerebellar development. Kitao, Y., Hashimoto, K., Matsuyama, T., Iso, H., Tamatani, T., Hori, O., Stern, D.M., Kano, M., Ozawa, K., Ogawa, S. J. Neurosci. (2004) [Pubmed]
  10. Identification of a cis-acting DNA antisilencer element which modulates vimentin gene expression. Stover, D.M., Zehner, Z.E. Mol. Cell. Biol. (1992) [Pubmed]
  11. Lipopolysaccharide-induced biphasic inositol 1,4,5-trisphosphate response and tyrosine phosphorylation of 140-kilodalton protein in mouse peritoneal macrophages. Shinji, H., Akagawa, K.S., Tsuji, M., Maeda, M., Yamada, R., Matsuura, K., Yamamoto, S., Yoshida, T. J. Immunol. (1997) [Pubmed]
  12. Amplification of the structurally and functionally altered epidermal growth factor receptor gene (c-erbB) in human brain tumors. Yamazaki, H., Fukui, Y., Ueyama, Y., Tamaoki, N., Kawamoto, T., Taniguchi, S., Shibuya, M. Mol. Cell. Biol. (1988) [Pubmed]
  13. axl, a transforming gene isolated from primary human myeloid leukemia cells, encodes a novel receptor tyrosine kinase. O'Bryan, J.P., Frye, R.A., Cogswell, P.C., Neubauer, A., Kitch, B., Prokop, C., Espinosa, R., Le Beau, M.M., Earp, H.S., Liu, E.T. Mol. Cell. Biol. (1991) [Pubmed]
  14. Activation of phospholipase C gamma in Schizosaccharomyces pombe by coexpression of receptor or nonreceptor tyrosine kinases. Arkinstall, S., Payton, M., Maundrell, K. Mol. Cell. Biol. (1995) [Pubmed]
  15. Purification of the murine interleukin 3 receptor. Mui, A.L., Kay, R.J., Humphries, R.K., Krystal, G. J. Biol. Chem. (1992) [Pubmed]
  16. Identification of a novel structural variant of the alpha 6 integrin. Davis, T.L., Rabinovitz, I., Futscher, B.W., Schnölzer, M., Burger, F., Liu, Y., Kulesz-Martin, M., Cress, A.E. J. Biol. Chem. (2001) [Pubmed]
  17. Nerve growth factor stimulates the activities of the raf-1 and the mitogen-activated protein kinases via the trk protooncogene. Ohmichi, M., Pang, L., Decker, S.J., Saltiel, A.R. J. Biol. Chem. (1992) [Pubmed]
  18. Entamoeba histolytica contains a beta 1 integrin-like molecule similar to fibronectin receptors from eukaryotic cells. Talamás-Rohana, P., Hernández-Ramirez, V.I., Perez-García, J.N., Ventura-Juárez, J. J. Eukaryot. Microbiol. (1998) [Pubmed]
  19. Protein tyrosine phosphorylation induced by epidermal growth factor and insulin-like growth factor-I in a rat clonal dental pulp-cell line. Kawase, T., Orikasa, M., Ogata, S., Burns, D.M. Arch. Oral Biol. (1995) [Pubmed]
  20. cDNA cloning of mouse ret proto-oncogene and its sequence similarity to the cadherin superfamily. Iwamoto, T., Taniguchi, M., Asai, N., Ohkusu, K., Nakashima, I., Takahashi, M. Oncogene (1993) [Pubmed]
  21. Induction of NCAM expression in mouse olfactory keratin-positive basal cells in vitro. Satoh, M., Takeuchi, M. Brain Res. Dev. Brain Res. (1995) [Pubmed]
  22. The c-kit receptor transduces the stem cell factor-triggered growth signal in murine interleukin-3-dependent cell line. Ohashi, H., Kameda, R., Nishikawa, M., Kawagishi, M., Liu, Y.C. Cytotechnology. (1994) [Pubmed]
  23. Dynamic anchoring of PKA is essential during oocyte maturation. Newhall, K.J., Criniti, A.R., Cheah, C.S., Smith, K.C., Kafer, K.E., Burkart, A.D., McKnight, G.S. Curr. Biol. (2006) [Pubmed]
  24. Characterization of the receptor for keratinocyte growth factor. Evidence for multiple fibroblast growth factor receptors. Bottaro, D.P., Rubin, J.S., Ron, D., Finch, P.W., Florio, C., Aaronson, S.A. J. Biol. Chem. (1990) [Pubmed]
  25. Localization and binding of transforming growth factor-beta isoforms in mouse preimplantation embryos and in delayed and activated blastocysts. Paria, B.C., Jones, K.L., Flanders, K.C., Dey, S.K. Dev. Biol. (1992) [Pubmed]
  26. A novel type of adhering junction in an epithelioid tumorigenic rat cell culture line. Schmelz, M., Way, D.L., Borgs, P., Peitsch, W.K., Schmidt, H., Witte, M.H., Witte, C.L., Franke, W.W., Moll, R. Cell Tissue Res. (1998) [Pubmed]
  27. Preferential binding of insulin-like growth factor-II (IGF-II) to a putative alpha 2 beta 2 IGF-II receptor type in C2 myoblasts. Domeyne, A., Pinset, C., Montarras, D., Garandel, V., Rosenfeld, R.G., Barenton, B. Eur. J. Biochem. (1992) [Pubmed]
  28. A non-sulfated form of the HNK-1 carbohydrate is expressed in mouse kidney. Tagawa, H., Kizuka, Y., Ikeda, T., Itoh, S., Kawasaki, N., Kurihara, H., Onozato, M.L., Tojo, A., Sakai, T., Kawasaki, T., Oka, S. J. Biol. Chem. (2005) [Pubmed]
  29. Rek, a gene expressed in retina and brain, encodes a receptor tyrosine kinase of the Axl/Tyro3 family. Biscardi, J.S., Denhez, F., Buehler, G.F., Chesnutt, D.A., Baragona, S.C., O'Bryan, J.P., Der, C.J., Fiordalisi, J.J., Fults, D.W., Maness, P.F. J. Biol. Chem. (1996) [Pubmed]
  30. Integrin alpha 7 as substrate for a glycosylphosphatidylinositol-anchored ADP-ribosyltransferase on the surface of skeletal muscle cells. Zolkiewska, A., Moss, J. J. Biol. Chem. (1993) [Pubmed]
  31. P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. Di Stefano, P., Cabodi, S., Boeri Erba, E., Margaria, V., Bergatto, E., Giuffrida, M.G., Silengo, L., Tarone, G., Turco, E., Defilippi, P. Mol. Biol. Cell (2004) [Pubmed]
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