Disease relevance of Hst1

• The Hybrid sterility 1 (Hst1) gene causes male infertility in crosses between certain inbred strains of the laboratory and wild mouse, Mus musculus [1].
• Hybrid sterility 1 (Hst1) is the major gene responsible for sterility of male hybrids between Mus musculus and certain laboratory strains [2].
• Hst1 was expressed in an undifferentiated mouse teratocarcinoma cell line, F9 [3].
• Complex Robertsonian translocation heterozygosities in the mouse have been used to test different hypotheses regarding the correlation between male hybrid sterility and chromosomal abnormality [4].

High impact information on Hst1

• Thus, in mouse interspecific hybrids, several different hybrid sterility genes that impair gametogenesis of the male hybrids have been described [5].
• Our data provide molecular evidence for a major divergence near the pseudoautosomal region, consistent with the hypothesis that hybrid sterility in these species results from abnormal pairing of sex chromosomes during male meiosis [6].
• Chromosomal and gene hybrid sterility might be interrelated because the attributes of chromosomal sterility, such as X-autosome associations, occur also in interspecies hybrids with genic sterility [7].
• Hitherto, five hybrid sterility (Hst) loci have been identified in the mouse genome [7].
• In an effort to further understand the role of Hst-1/FGF-4 in mouse limb development, an antisense oligodeoxynucleotides (ODNs) study was performed [8].

Chemical compound and disease context of Hst1

• All-trans-retinoic acid and hexamethylene bisacetamide (HMBA) regulate TGF-alpha and Hst-1/kFGF expression in differentiation sensitive but not in resistant human teratocarcinomas [9].

Biological context of Hst1

• The map contains two phenotypic loci, T and Hst1, 12 RFLP markers, and 24 microsatellite loci [10].
• Sub-milliMorgan map of the proximal part of mouse Chromosome 17 including the hybrid sterility 1 gene [10].
• Each recombinant was also progeny-tested to ascertain its Hybrid sterility-1 (Hst-1) genotype by crossing to PWB/Ph, a Mus musculus-derived inbred strain [11].
• We found that mouse Hst1 and Int-2, like their human counterparts, were located close to each other on the genome: the distance was less than 20 kbp [3].
• Differential expression of two homologous and clustered oncogenes, Hst1 and Int-2, during differentiation of F9 cells [3].

Anatomical context of Hst1

• Using this organ culture system, we have shown that exposure of 9.5-10-d p.c. embryonal limb bud explants to antisense ODNs of Hst-1/FGF-4 blocks limb development [8].
• Detection of spatial localization of Hst-1/Fgf-4 gene expression in brain and testis from adult mice [12].
• The X-linked hybrid sterility is associated with reduced testes weight, lower sperm count, and morphological abnormalities of sperm heads [13].

Other interactions of Hst1

• To identify the causative gene, we have searched YAC clones encompassing the Hst1 region for testis-expressed sequences, using the cDNA selection method [1].
• Genetic mapping of the t-complex region on mouse chromosome 17 including the Hybrid sterility-1 gene [11].
• Here we report the identification and characterization of two additional hybrid sterility loci, Hst-5 and Hst-6, that map to the same inversion [14].
• In previous studies, a locus responsible for hybrid sterility in the genus Mus,Hst-4, was mapped to the distal inversion of the t complex on chromosome 17 [14].
• Through an analysis of recombinant chromosomes, it was possible to map the M. domesticus, M. spretus and t haplotype alleles responsible for these two hybrid sterility phenotypes to the same novel locus (Hybrid sterility-4) [15].

Analytical, diagnostic and therapeutic context of Hst1

• In the present study, as a first step to clarify the mechanism of hybrid sterility caused by the Hst-1 gene in mice, the male sterile hybrid between C57BL/6 and M. m. musculus was investigated by histological techniques [16].
• In situ hybridization technique was used to localize Hst-1/Fgf-4 gene expression in the cerebellum and testis from 10-week-old mice [12].
• To investigate whether there is a possible role of HST-1/FGF-4 in adult stage, we have carried out a highly sensitive RT-PCR analysis of Hst-1/Fgf-4 gene expression in adult mice tissues [12].
• Identification and linkage mapping of Hst7, a new M. spretus/M. m. domesticus chromosome 17 hybrid sterility locus [17].

References