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Chemical Compound Review

ethylmaleimide     1-ethylpyrrole-2,5-dione

Synonyms: N-EM, Lopac-E-3876, CHEMBL8211, SureCN19666, NSC-7638, ...
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Disease relevance of ethylmaleimide


Psychiatry related information on ethylmaleimide

  • The effects on olfaction of N-ethylmaleimide (NEM), a specific reagent of free sulfhydryl groups, were studied in the moth Mamestra brassicae [6].
  • IM significantly attenuated the inhibitory effect of AZ on the motor activity, while NEM did not affect the inhibited motor responses caused by AZ and Cys [7].

High impact information on ethylmaleimide


Chemical compound and disease context of ethylmaleimide


Biological context of ethylmaleimide

  • In normal subjects, this system was resistant to N-ethylmaleimide and demonstrated saturation kinetics [17].
  • Here we show that the neural t-SNARE (target-membrane-associated-soluble N-ethylmaleimide fusion protein attachment protein (SNAP) receptor) SNAP-25 is not required for nerve growth or stimulus-independent neurotransmitter release, but is essential for evoked synaptic transmission at neuromuscular junctions and central synapses [18].
  • The spontaneous or diamide-induced formation of disulfide bonds between cysteines 437 and 503 or 437 and 506, in apo-IRF, as well as its alkylation by N-ethylmaleimide, inhibit binding to the IRE.(ABSTRACT TRUNCATED AT 250 WORDS)[19]
  • Moreover, NEM modification also indicates an additional tRNA binding site on EF-Tu.GTP.kirromycin, which could not be detected with TPCK [20].
  • From the calcium dependence of cortical granule exocytosis, and from the exposure time and concentration dependence of N-ethylmaleimide inhibition, we determined that cortical granules do have spare active fusion complexes that are randomly distributed as a Poisson process among the population of granules [21].

Anatomical context of ethylmaleimide


Associations of ethylmaleimide with other chemical compounds


Gene context of ethylmaleimide


Analytical, diagnostic and therapeutic context of ethylmaleimide

  • In the second group, the addition of N-ethylmaleimide (10 microM), a Gi/Go protein antagonist, abolished AngII- induced EA (n = 6) but not AA constriction (n = 6) [36].
  • Moreover, immunoprecipitation of elastase extracts alkylated with [14C] N-ethylmaleimide selected a single species of mol wt 60,000 [37].
  • Neither microinjection of phalloidin, DNase I or N-ethylmaleimide-modified heavy meromyosin nor exogenous application of cytochalasin B has any effect on pigment motion, although these materials do block the actin-mediated motility of other systems in our hands [38].
  • In a preliminary biochemical dissection of the system, we observe that the sulfhydryl alkylating reagent N-ethylmaleimide inactivates both cytosolic factor(s) and also component(s) in the insoluble permeabilized cell fraction required for nuclear protein import [39].
  • In a murine heart perfusion system, we were able to "turn off" the transport of derivatized albumin [dinitrophenylated albumin (DNP-albumin)] from the perfusate to the tissue, by preperfusing the system with 1 mM N-ethylmaleimide (NEM) for 5 min at 37 degrees C, followed by a 5-min perfusion of DNP-albumin in the presence of NEM [40].


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