High impact information on Csnk1e

• We conclude that tau is an allele of hamster CKIepsilon and propose a mechanism by which the mutation leads to the observed aberrant circadian phenotype in mutant animals [1].
• tau protein isolated from porcine brain microtubules was further purified by electrophoretic elution from polyacrylamide gels and used to prepare antisera in rabbits [2].
• Control of mammalian circadian rhythm by CKIepsilon-regulated proteasome-mediated PER2 degradation [3].
• We thus propose that the CKIepsilon-binding domain is critical not only for mPER phosphorylation but also for a functioning circadian clock [4].
• In vitro studies with chimeric proteins suggest that the inability of mPER3 to support circadian clock function results in part from lack of direct and stable interaction with casein kinase Iepsilon (CKIepsilon) [4].

Biological context of Csnk1e

• cDNA cloning and chromosome mapping of the mouse casein kinase I epsilon gene (Csnk1e) [5].
• Csnk1e is a key component in the Darpp-32 (Dopamine-And-cAMP-Regulated-Phosphoprotein-32 kDa) second messenger pathway and has been implicated in previous pharmacological and pharmacogenetic studies in mice [6].
• Here, we provide the first evidence that CKIepsilon is involved in hematopoietic cell differentiation [7].
• Casein kinase I delta (CKIdelta) and casein kinase I epsilon (CKIepsilon) have been implicated in the response to DNA damage, but the understanding of how these kinases are regulated remains incomplete [8].

Anatomical context of Csnk1e

• Control of intracellular dynamics of mammalian period proteins by casein kinase I epsilon (CKIepsilon) and CKIdelta in cultured cells [9].
• Thus, the suppression of CKIepsilon in many human leukemia cell lines may play a role in the cell immortalization [10].

Associations of Csnk1e with chemical compounds

• Mice bred for methamphetamine sensitivity showed linkage to the region of chromosome 15 that contains Csnk1e and also showed a 10-fold increase in expression of Csnk1e [6].
• These findings demonstrate a novel role for CKIepsilon in the regulation of Ror2 tyrosine kinase [11].
• Furthermore, it was found that association of Ror2 with CKIepsilon is required for its serine/threonine phosphorylation by CKIepsilon [11].
• We also show that Ror2 is phosphorylated by CKIepsilon on serine/threonine residues, in its C-terminal serine/threonine-rich 2 domain, resulting in autophosphorylation of Ror2 on tyrosine residues [11].

Regulatory relationships of Csnk1e

• These results suggest that CKIepsilon and CKIdelta regulate the mammalian circadian autoregulatory loop by controlling both protein turnover and subcellular localization of mPer proteins [9].
• Moreover, CKIepsilon and CKIdelta are able to induce nuclear translocation of mPer3, which requires its nuclear localization signal [9].

Other interactions of Csnk1e

• Coexpression of XWnt-8 or addition of soluble Wnt-3a ligand led to a significant and rapid increase in the activity of endogenous CKIepsilon [12].
• We show that when expressed in mammalian cells, Ror2 associates with casein kinase Iepsilon (CKIepsilon), a crucial regulator of Wnt signaling [11].
• CKIepsilon and CKIdelta affected the inhibitory effect of mPer proteins on the transcriptional activity of BMAL1-CLOCK, but the inhibitory effect of mCry proteins on the activity of BMAL1-CLOCK was unaffected [9].

Analytical, diagnostic and therapeutic context of Csnk1e

• Animal models suggest that the casein kinase 1 epsilon gene (Csnk1e) contributes to variability in stimulant response [6].

References