High impact information on ZFY

• ZFY transcripts were detected in unsexed pools of 8-16-cell stage embryos and in male blastocysts [1].
• ZFY is highly homologous with ZFX and, as a result, ZFY could be mapped simultaneously [2].
• ZFY was mapped to the distal region of the short arm of the Y Chromosome (Chr) (Yp13), contradicting the previously reported position Yq1 [2].
• Cloning and mapping of bovine ZFX gene to the long arm of the X-chromosome (Xq34) and homologous mapping of ZFY gene to the distal region of the short arm of the bovine (Yp13), ovine (Yp12-p13), and caprine (Yp12-p13) Y chromosome [2].
• We have examined mRNA expression of two genes located on the Y chromosome, the sex-determining region Y gene (SRY) and the linked zinc finger gene (ZFY), using in vitro fertilized-in vitro cultured bovine embryos [3].

Biological context of ZFY

• One sample of each original embryo was diagnosed for sex, based on restriction fragment length polymorphism of PCR-amplified DNA of the ZFX/ZFY locus [4].
• The quarter-embryos were sexed by polymerase chain reaction (PCR) using BRY.1, Bov97M and ZFX/ZFY loci, and then genotyped for k-casein, bovine leukocyte adhesion deficiency (BLAD) and microsatellite D9S1 [5].
• Like in humans, both bovine ZFY and ZFX contain 13 zinc finger motifs and belong to the Krueppel family of C2H2-type zinc finger proteins [6].
• Using comparative sequencing, single base pair polymorphisms between the amplified ZFY and ZFX were established [7].
• Sex and the kappa-casein genotype of in vitro produced cattle demiembryos was identified by simultaneous PCR-genotyping using polymorphic sites in ZFY/ZFX and kappa-casein genes [8].

Associations of ZFY with chemical compounds

• Ovine and caprine ZFY were also mapped with bovine ZFX [2].

Other interactions of ZFY

• Because male ovine embryos develop faster than female embryos, the transcription of SRY and ZFY, two genes located on the Y chromosome, was examined in preimplantation stages using the reverse transcriptase polymerase chain reaction (RT-PCR) [9].

Analytical, diagnostic and therapeutic context of ZFY

• It was ascertained that the two genes, ZFY and AMG gene which located on the short arm of the Y chromosome (Yp) were detected in normal bulls and a XY female, but were not detected in normal cow, mother cow and half-sib heifer by Southern blotting [10].

References