High impact information on SRY

• Porcine SRY HMG-box related gene-9 (pSOX-9) expression also paralleled that of pSF-1 in developing testes [1].
• These domains may have a function in the control of SRY expression during fetal development [2].
• Unlike the human SRY promoter, CAAT and TATA box motifs are present in the bovine sequences [3].
• The PCR utilized two different sets of primers: the first pair recognized a bovine Y-chromosome-specific sequence (SRY), that showed 100% homology with the corresponding sequence of the ovine Y-chromosome and is amplified in males only [4].
• Cytogenetic and fluorescence in situ hybridization analysis of cell cultures as well as prove of the presence of the SRY gene sequence revealed a heterosexual twin pregnancy [5].

Biological context of SRY

• Three genes, SRY, ANT3, and CSF2RA, were mapped to the bovine Y chromosome (BTAY) by fluorescence in situ hybridization (FISH) and/or radiation hybrid (RH) mapping [6].
• Male-specific SRY and ZFY haplotypes in US beef cattle [7].
• C- and R-banding comparison were also performed and both bovine and caprine BAC clones containing the SRY, ZFY, UMN0504, UMN0301, UMN0304 and DYZ10 loci in cattle and DXYS3 and SLC25A6 in goat were hybridized on R-banded chromosomes by FISH [8].
• Amplification and analysis of a male-specific SRY homologous sequence of cattle [9].
• Phylogenies using mtDNA and SRY provide evidence for male-mediated introgression in Asian domestic cattle [10].

Anatomical context of SRY

• Northern analysis of bull testicular RNA demonstrated low levels of expression of the bovine SRY gene in adult testes with a major poly(A) species at 1.9 kb [3].
• Early transcription of the SRY gene by bovine preimplantation embryos [11].
• Expression of the SRY gene, implicated in sex determination in mammals, has been reported to occur both for a short time at the sex-determining stage of development around the period of the primitive undifferentiated gonad and in the adult testis [11].
• The log ratio (SRY/F9) of the corrected intensity densities were used to estimate the percent Y-chromosome DNA bearing spermatozoa (%Y-CDBS) in each ejaculate using inverse regression procedures [12].
• Weights for total dissectible (TDF) and subcutaneous fat (SQ) from each of nine wholesale cuts were recorded [13].

Associations of SRY with chemical compounds

• Ethidium bromide stained bands were detected by image analysis and compared to a standard curve constructed from pure SRY and F9 PCR product [12].
• The Mishima, Mongolian, Korean, Chinese Yellow and Sri Lanka cattle populations had a full match between the mtDNA and SRY sequences, specifically the taurine/taurine type or zebu/zebu type [10].

Other interactions of SRY

• ZFY and ZFX transcripts were present in all stages examined following RT-PCR, whereas transcripts for SRY were undetectable in all investigated stages following either RT nested PCR or Southern analysis [14].

Analytical, diagnostic and therapeutic context of SRY

• The bovine SRY gene was cloned by a combination of anchored polymerase chain reaction (PCR) amplification of genomic restriction fragments and reverse transcription-PCR (RT-PCR) of testicular RNA [3].
• In this study, using a sensitive reverse transcriptase-polymerase chain reaction (RT-PCR) assay, we detected SRY but not ZFY mRNA expression as early as the 4- to 8-cell stage and through to the blastocyst stage in bovine embryos [11].

References