High impact information on ZFX

• Comparison of substitution rates in ZFX and ZFY introns of sheep and goat related species supports the hypothesis of male-biased mutation rates [1].
• G6PD, HPRT and ZFX transcripts were noted in all pre-compaction stage embryos and in female and male blastocysts [2].
• The expression of ZFX was also significantly higher in female than in male blastocysts [2].
• ZFY is highly homologous with ZFX and, as a result, ZFY could be mapped simultaneously [3].
• The bovine ZFX consisted of 5328 bp and encoded 800 amino acid residues, which contained 13 zinc finger motifs [3].

Biological context of ZFX

• One sample of each original embryo was diagnosed for sex, based on restriction fragment length polymorphism of PCR-amplified DNA of the ZFX/ZFY locus [4].
• The quarter-embryos were sexed by polymerase chain reaction (PCR) using BRY.1, Bov97M and ZFX/ZFY loci, and then genotyped for k-casein, bovine leukocyte adhesion deficiency (BLAD) and microsatellite D9S1 [5].
• Cloning and mapping of bovine ZFX gene to the long arm of the X-chromosome (Xq34) and homologous mapping of ZFY gene to the distal region of the short arm of the bovine (Yp13), ovine (Yp12-p13), and caprine (Yp12-p13) Y chromosome [3].
• Ovine ZFX was mapped to a region close to the centromere of the X Chr (Xq13) [3].
• Using comparative sequencing, single base pair polymorphisms between the amplified ZFY and ZFX were established [6].

Associations of ZFX with chemical compounds

• Ovine and caprine ZFY were also mapped with bovine ZFX [3].

Other interactions of ZFX

• ZFY and ZFX transcripts were present in all stages examined following RT-PCR, whereas transcripts for SRY were undetectable in all investigated stages following either RT nested PCR or Southern analysis [7].

Analytical, diagnostic and therapeutic context of ZFX

• ZFX was used as a probe for fluorescence in situ hybridization and was mapped to Xq34, different from its previously reported site at Xq21-q231 [3].

References