Disease relevance of OSGIN1

• Our data suggest the potential use of OKL38 in diagnosis, prognosis, and/or treatment of kidney cancer [1].
• However, low levels of OKL38 transcripts were observed in the various human breast cancer cell lines studied and were barely detectable in all dimethylbenz(A)anthracene-induced mammary tumors examined [2].
• To develop a rat model for further characterization of OKL38's role in the initiation and progression of breast and ovarian cancer, we now report the cloning and characterization of three novel rat OKL38 cDNAs that are derived through alternative splicing and differential promoter usage [3].

High impact information on OSGIN1

• The human OKL38 gene spans approximately 18 kb and contains 8 exons and 7 introns with exon size ranging from 92 to 1270 bp [1].
• A transfection study using OKL38-eGFP recombinant construct showed that overexpression of the 52 kDa OKL38 protein in A498 cells resulted in growth inhibition and cell death [1].
• Multiple Tissue Expression array (MTE) and Multiple Tissue Northern blot (MTN) indicated that OKL38 was ubiquitously expressed in all tissues with high expression in liver, kidney, and testis [1].
• Two genes were consistently overexpressed: OKL38 and CDH13 [4].
• In situ hybridization localized the rat OKL38 transcripts to the luminal epithelial cells of the rat mammary gland and to the granulosa cells in the rat ovary [3].

Biological context of OSGIN1

• Structural characterization of three novel rat OKL38 transcripts, their tissue distributions, and their regulation by human chorionic gonadotropin [3].
• Here we report the cloning and characterization of a novel pregnancy-induced cDNA, OKL38, from a human ovarian cDNA library [2].
• The onset and advancement of pregnancy also gave rise to a concomitant increase in OKL38 gene expression [2].
• OKL38 is mapped to chromosome 19, spanning a region of approximately 15 kb, and contains eight exons [3].

Anatomical context of OSGIN1

• These observations led to speculation that OKL38 may play a vital role in the growth regulation and differentiation of breast epithelial cells during pregnancy and its implications in tumorigenesis [2].
• Differential expression of these three rat OKL38 transcripts was observed in liver, kidney, ovary, mammary gland, and uterus [3].

Analytical, diagnostic and therapeutic context of OSGIN1

• In situ hybridization revealed that OKL38 mRNA was further detected in mammary secretory epithelial cells [2].

References