Disease relevance of DnaJ-1

• Negative regulation of hepatitis B virus replication by cellular Hsp40/DnaJ proteins through destabilization of viral core and X proteins [1].
• A partial open reading frame 165 base pairs 3' to the end of dnaK encodes a peptide of 190 amino acids that is 59% identical to DnaJ of E. coli [2].
• Unexpectedly, cystamine treatment increased transcription of one of the two genes shown to be neuroprotective for polyglutamine toxicity in Drosophila, dnaj (also known as HDJ1 and Hsp40 in humans and mice, respectively) [3].
• Understanding human cancer using Drosophila: Tid47, a cytosolic product of the DnaJ-like tumor suppressor gene l2Tid, is a novel molecular partner of patched related to skin cancer [4].

High impact information on DnaJ-1

• The DnaJ-like cysteine string protein and exocytotic neurotransmitter release [5].
• Overexpression of DROJ1 in Drosophila SL2 cells delays the onset of the heat shock response [6].
• Moreover, RNA interference involving transfection of SL2 cells with double-stranded droj1 RNA depletes the endogenous level of DROJ1 protein, leading to constitutive activation of endogenous heat shock genes [6].
• Tid1, a DnaJ cochaperone protein, is the mammalian homologue of the Drosophila tumor suppressor Tid56 whose antitumor function is most likely mediated through its capacity to regulate cell differentiation in imaginal discs [7].
• In the photoreceptors, expression of another suppressor with a J domain, dHDJ1, but not dMRJ, prior to expression of expanded polyQs dramatically promoted cytoplasmic aggregation [8].

Biological context of DnaJ-1

• Thermoprotection of synaptic transmission in a Drosophila heat shock factor mutant is accompanied by increased expression of Hsp83 and DnaJ-1 [9].

Anatomical context of DnaJ-1

• We recovered cell lines containing between three and about 50 copies of both pCV31gpt and pMH10A, a cloned 87 A7 hsp70 heat-shock locus that encodes a mutant 40,000-dalton heat-shock protein (hsp40) [10].

Other interactions of DnaJ-1

• The Drosophila melanogaster tumor suppressor gene lethal(2)tumorous imaginal discs (tid) was identified as a homolog of all dnaJ-like genes known to date which have been well preserved in evolution [11].

Analytical, diagnostic and therapeutic context of DnaJ-1

• In addition, increases in the levels of Hsp83 and DnaJ-1 proteins but not in the inducible form of Hsp70 were detected by Western blot analysis [9].
• Binding of the viral core protein with the Hsp40 proteins was confirmed by affinity chromatography and immunoprecipitation of transiently expressed proteins [1].

References