Psychiatry related information on Crc

• To further elucidate the molecular basis of olfaction, we explored the role of the calcium-binding chaperone calreticulin in the behavioral response of Drosophila to aversive odorants [1].

High impact information on Crc

• The amino acid sequence of rabbit calreticulin is 92% homologous to both murine calreticulin and human Ro [2].
• We have isolated a gain-of-function allele of let-60 ras that confers a Clr phenotype and implicated both let-60 ras and components of a mitogen-activated protein kinase cascade in EGL-15 signaling by their Soc phenotype [3].
• In Drosophila, calreticulin seems to mediate these anesthetic sensitivities, and it is a possible target for diethylether and isoflurane, although the predicted anesthetic targets based on many studies in vitro and in vivo are the membrane proteins, such as ion channels and receptors [4].
• For anesthetic phenotype assay, the 50% anesthetizing concentrations were determined for ethas311, revertants, and double-mutant strains (wild-type crc transgene plus ethas311) [4].
• In addition, we will describe insect and mammalian expression systems in which major histocompatibility complex class I molecules are used as model glycoprotein substrates for CNX and CRT [5].

Biological context of Crc

• Nucleotide sequence of a Drosophila melanogaster gene encoding a calreticulin homologue [6].
• The mouse cDNA-encoded amino acid sequence was found to be 94% homologous to the human Ro sequence and is 100% homologous to murine calreticulin, a high affinity calcium-binding protein which resides in the endoplasmic and sarcoplasmic reticulum [2].
• In vitro and in vivo assays to assess the functions of calnexin and calreticulin in ER protein folding and quality control [5].
• The number and size of CRT introns have increased through the evolution of eukaryotes [7].
• The CRT gene product, calreticulin, was first identified as a calcium binding protein in 1974, but further investigations have indicated that CRT protein performs many functions in cells, including involvement in evading the host's immune system by parasites [7].

Anatomical context of Crc

• Calreticulin is known as a multifunctional calcium-binding protein in the endoplasmic reticulum of non-muscle cells [8].
• Calreticulin mutants have broad defects in odor avoidance without abnormalities in antennal responses to odorants, alterations in central nervous system structure, or deficits in overall locomotor abilities [1].
• The highest level of mRNA expression of B. mori calreticulin was exhibited in the fat body of this insect [9].

Analytical, diagnostic and therapeutic context of Crc

• METHODS: Molecular analysis of crc included cloning and sequencing of the cDNA, Northern blotting, and in situ hybridization to accomplish the function of the gene and its mutation [4].

References