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Gene Review:

LUM - lumican

Gallus gallus

Blochberger, T.C. et al., Sundarraj, N. et al., Hassell, J.R. et al., Corpuz, L.M. et al., Dunlevy, J.R. et al., et al.

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High impact information on LUM

Results showed that all of the peptides sequenced aligned to the deduced amino acid sequence of either chick lumican or chick keratocan at the first, third, and fourth potential N-linked sites [1].
A 1.9-kb cDNA clone to chick lumican (keratan sulfate proteoglycan) was isolated by screening an expressing vector library made from chick corneal RNA with antiserum to chick corneal lumican [2].
cDNA to chick lumican (corneal keratan sulfate proteoglycan) reveals homology to the small interstitial proteoglycan gene family and expression in muscle and intestine [2].
Lumican contains three variations of this sequence that are tandemly linked to form a unit and three units tandemly linked to form the leucine-rich region [2].
Isolation and partial characterization of lumican and decorin from adult chicken corneas. A keratan sulfate-containing isoform of decorin is developmentally regulated [3].

Biological context of LUM

These results show the chick lumican gene to consist of three exons, and that regulatory elements are present within 1 kb upstream of the first exon [4].

Anatomical context of LUM

Both lumican and mimecan are expressed at lowest levels in brain, liver and sternum [5].
The corneas were harvested at different times after wounding and processed for either histochemical analyses using periodic acid-Schiff's reaction (PAS) or for indirect immunofluorescence analyses of lumican, keratocan, keratan sulfate, perlecan and laminin [6].
Decorin is highly expressed in sclera and sternum, whereas lumican is expressed in these tissues, as well as in liver, at very low levels [7].

Analytical, diagnostic and therapeutic context of LUM

Radiolabeling and immunoprecipitation studies show that lumican core protein is also synthesized by these tissues [2].
No differences were evident in the immunofluorescence for keratocan or keratan sulfate in the scar tissue, but the scar tissue did contain markedly decreased levels of lumican [6].
Molecular cloning and relative tissue expression of decorin and lumican in embryonic quail cornea [7].
Three overlapping genomic clones to chick lumican were isolated and then characterized using restriction enzyme analyses, Southern blot analyses with cDNA probes, and by DNA sequencing [4].

References

Identification of the N-linked oligosaccharide sites in chick corneal lumican and keratocan that receive keratan sulfate. Dunlevy, J.R., Neame, P.J., Vergnes, J.P., Hassell, J.R. J. Biol. Chem. (1998) [Pubmed]
cDNA to chick lumican (corneal keratan sulfate proteoglycan) reveals homology to the small interstitial proteoglycan gene family and expression in muscle and intestine. Blochberger, T.C., Vergnes, J.P., Hempel, J., Hassell, J.R. J. Biol. Chem. (1992) [Pubmed]
Isolation and partial characterization of lumican and decorin from adult chicken corneas. A keratan sulfate-containing isoform of decorin is developmentally regulated. Blochberger, T.C., Cornuet, P.K., Hassell, J.R. J. Biol. Chem. (1992) [Pubmed]
Gene structure of chick lumican and identification of the first exon. Hassell, J.R., Rada, J., Cornuet, P., Vergnes, J.P., Kinchington, P.R. Biochim. Biophys. Acta (1998) [Pubmed]
Molecular cloning and relative tissue expression of keratocan and mimecan in embryonic quail cornea. Corpuz, L.M., Dunlevy, J.R., Hassell, J.R., Conrad, A.H., Conrad, G.W. Matrix Biol. (2000) [Pubmed]
Proteoglycan distribution during healing of corneal stromal wounds in chick. Sundarraj, N., Fite, D., Belak, R., Sundarraj, S., Rada, J., Okamoto, S., Hassell, J. Exp. Eye Res. (1998) [Pubmed]
Molecular cloning and relative tissue expression of decorin and lumican in embryonic quail cornea. Corpuz, L.M., Dunlevy, J.R., Hassell, J.R., Conrad, A.H., Conrad, G.W. Matrix Biol. (2000) [Pubmed]

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