Disease relevance of Periodic Acid-Schiff Reaction

• In a series of 78 cases of acute lymphocytic leukemia, seven were found to demonstrate blast cells staining strongly for both the periodic acid-Schiff reaction and alpha-naphthyl acetate esterase [1].
• Sulfated mucins were found in five of 10 cases of infiltrating lobular carcinoma, and in six of 10 cases there was a positive periodic acid-Schiff reaction following KOH/PBT [2].
• Similarly in some gastric cancers which do not stain with the periodic acid-Schiff reaction at all, large amount of free or neuraminic acid substituted PNA binding sites can be detected [3].
• At 1.0 MgCl2, metaphyseal dysostosis plates are weakly periodic acid-Schiff (PAS) positive compared to intense PAS reactions in normal growth plates [4].
• The intensity of these reactions can be greatly enhanced by using 3 steps in tissue preparation, either singly or in combination: 1) The PAS reaction in semithin sections turns out stronger after partial (70% ethanol) than complete (100% ethanol) dehydration of the tissue before its transfer to 100% LR White [5].

High impact information on Periodic Acid-Schiff Reaction

• This lineage was found to have alpha 1-Pi-induced liver pathology in the neonatal period, concomitant with the accumulation of human Z protein in diastase-resistant cytoplasmic globules that could be revealed in the Periodic acid-Schiff reaction (PAS) [6].
• It stains intensely with Coomassie blue, but gives negative reactions to carbohydrate (periodic acid/Schiff reaction) and polyanions (alcian blue, colloidal iron, and carbocyanine) [7].
• Other enzyme histochemical and lectin-binding studies showed that the enzyme alpha-naphthyl acetate esterase, the lectin Ulex europaeus I, and the periodic-acid Schiff reaction were consistent, but not specific, markers of megakaryocytes [8].
• Cytochemical evaluation was performed, including periodic acid-Schiff reaction, Sudan black B, alpha-naphthyl acetate, alpha-naphthyl butyrate, naphthol AS-D chloroacetate, and acid phosphatase stains [9].
• MEG-01 cells were positive for the periodic acid-Schiff reaction, alpha-naphthyl acetate esterase, and acid phosphatase, and negative for myeloperoxidase, alpha-naphthyl butyrate esterase, naphthol AS-D chloroacetate esterase, and alkaline phosphatase [10].

Biological context of Periodic Acid-Schiff Reaction

• Cell proliferation was detected with Ki-67 immunostaining and proliferating cells were identified with vimentin as glial and with CD31 and periodic acid-Schiff (PAS) reaction as endothelial elements [11].
• Cytochemistry evaluation showed that MSCs were homogeneously positive for acid alpha-naphthl acetate esterase (ANAE), glycogen (periodic acid Schiff reaction, PAS), and negative for acid phosphatase (ACP) and the Sudan black reaction (SB) [12].
• Features of megakaryocytopoiesis were determined not only by using the periodic acid Schiff reaction (PAS), but also by immunostaining with the monoclonal antibody Y2/51, detecting a formalin-resistant epitope of glycoprotein IIIa (CD61) [13].

Anatomical context of Periodic Acid-Schiff Reaction

• In the dermal papillae where amyloid was deposited, elastic fibers frequently were absent, but periodic acid-Schiff reaction after diastase digestion was homogenously positive [14].
• Samples of small intestine with luminal contents were frozen and sectioned in a cryostat for fluorescence microscopy; the same sections were placed in a 0.2% celloidin solution to preserve mucous gel and were stained by periodic acid-Schiff reaction for light microscopy [15].
• Semithin sections, made possible by infiltration and embedding in glycol methacrylate, were stained with hematoxylin and eosin to assess the general architecture of the organ and the lipid content of the hepatocytes and by the periodic acid-Schiff reaction and hematoxylin to visualize the cytoplasmic stores of glycogen [16].
• Blood and bone marrow cells of ten clinically healthy cats were stained for alkaline phosphatase (ALP), peroxidase (PO), chloroacetate esterase (CAE), alpha-naphthyl butyrate esterase (NBE), sudanophilia, and periodic acid-Schiff (PAS) reaction [17].
• Alcian blue (AB) staining and periodic acid-Schiff (PAS) reactions demonstrated that minipig submandibular glands synthesized and secreted acid mucous substances by serous cells and polysaccharide, and neutral mucous substances, by mucous cells [18].

Associations of Periodic Acid-Schiff Reaction with chemical compounds

• A number of glycoprotein bands which were undetected by protein stains or the periodic acid Schiff reaction were revealed by lectins [19].
• The morphologic appearance of the leukemic cells coupled with the results of cytochemical staining reactions (alpha-naphthyl AS-D chloroacetate esterase, alpha-naphthyl butyrate esterase, Sudan black B, and periodic acid-Schiff reaction) identified this leukemia as myelomonocytic in origin [20].
• These HBcAg inclusions stain greyish pink with chromotrope aniline blue and are negative for orcein, the periodic acid-Schiff reaction, and the Feulgen reaction for DNA [21].
• They were sometimes identified by haematoxylin and eosin and periodic acid Schiff reaction, but were not labeled by phosphotungstic acid haematoxylin or by antibodies to glial fibrillary acidic protein [22].
• The proportion of glycogen-depleted fibres (estimated from the periodic acid Schiff reaction, PAS) in muscles removed from animals 10 min after a 5 min period of isometric twitches was significantly lower in ischaemic muscles (45.1 +/- 1.9%) than in control (80.5 +/- 1.5%) or chronically stimulated ischaemic muscles (67.3 +/- 4.0%) [23].

Gene context of Periodic Acid-Schiff Reaction

• Serial frozen sections of longissimus dorsi muscles from twelve Canadian Yorkshire breed pigs at different live weights (13-86 kg) were stained for glycogen by the periodic acid-Schiff (PAS) reaction and reacted for NAD tetrazolium reductase to determine the mitochondrial content of muscle fibres [24].
• Histochemical staining procedures, including the periodic acid-Schiff (PAS) reaction, Alcian blue stain, and the colloidal iron stain (Müller's reagent), were investigated for their potential to identify mucin deposits on worn hydrogel contact lenses [25].
• Periodic acid-Schiff reaction with and without diastase digestion demonstrated the presence of glycogen and other carbohydrates, but histochemical stains for normal collagen, elastin and fibrin showed a weak variable reactivity of the deposit [26].
• In this study, all the CK isoenzymes demonstrated positive periodic acid-Schiff (PAS) reactions, indicating the presence of carbohydrate [27].
• The following cytochemical methods were used: acid phosphatase, lysozyme, peroxidase, alpha-naphthyl acetate esterase, alpha-naphthyl butyrate esterase, adenosine triphosphatase, and the periodic acid-Schiff reaction (PAS reaction) [28].

Analytical, diagnostic and therapeutic context of Periodic Acid-Schiff Reaction

• Immediately after renal scintigraphy, blood urea nitrogen and serum creatinine levels were measured and renal tissues stained by periodic acid Schiff reaction were examined in each group [29].

References