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Gene Review:

MTIF2 - mitochondrial translational initiation...

Homo sapiens

Synonyms: IF-2(Mt), IF-2Mt, IF-2mt, IF2(mt), Translation initiation factor IF-2, mitochondrial

Overman, R.G. et al., Hayashi, R. et al., Ma, L. et al., Bonner, D.S. et al., Ma, J. et al.

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Disease relevance of MTIF2

The sequence of bovine IF-2mt exhibits 85% identity to human IF-2mt, but only 38% identity to yeast IF-2mt and 39% identity to Escherichia coli IF-2 alpha [1].

High impact information on MTIF2

Complete cDNAs encoding human mitochondrial translational initiation factor 2 (IF-2mt) have been obtained from liver, heart, and fetal brain cDNA libraries [2].
Assignment1 of the mitochondrial translational initiation factor 2 gene (MTIF2) to human chromosome 2 bands p16-->p14 by in situ hybridization and with somatic cell hybrids [3].
The minimal promoter region for the human IF2mt gene contains binding sites for Nuclear Respiratory Factor 2 (NRF-2), which is often involved in the transcription of mitochondrial-related genes [4].
We also discovered a pseudogene of MTIF2 and mapped it to chromosome 1p13-12 [5].
Mitochondrial translation initiation factor 2 (MTIF2) is nuclear-encoded and functions in mitochondria to initiate the translation of proteins encoded by the mitochondrial genome [5].

Biological context of MTIF2

The human mitochondrial translation initiation factor 2 gene (MTIF2): transcriptional analysis and identification of a pseudogene [5].
MTIF2 is approximately 33.5-kb long and contains 16 exons, confirming data from the Human Genome Project. With RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE), we mapped the transcription start point in human heart tissue to a cytosine residue 296 bp upstream from the translation initiation site [5].
To gain insight into mechanisms that regulate MTIF2 gene expression, the genomic copy and the 5' and 3' flanking regions of MTIF2 were isolated using a combination of genomic library screening and polymerase chain reaction (PCR) [5].

Analytical, diagnostic and therapeutic context of MTIF2

Cloning and sequence analysis of the human mitochondrial translational initiation factor 2 cDNA [2].
Electrophoresis mobility shift assay (EMSA) analyses indicated that NRF-2alpha/beta binds to the IF2mt promoter [4].

References

Cloning and sequence analysis of the cDNA for bovine mitochondrial translational initiation factor 2. Ma, J., Farwell, M.A., Burkhart, W.A., Spremulli, L.L. Biochim. Biophys. Acta (1995) [Pubmed]
Cloning and sequence analysis of the human mitochondrial translational initiation factor 2 cDNA. Ma, L., Spremulli, L.L. J. Biol. Chem. (1995) [Pubmed]
Assignment1 of the mitochondrial translational initiation factor 2 gene (MTIF2) to human chromosome 2 bands p16-->p14 by in situ hybridization and with somatic cell hybrids. Bonner, D.S., Wiley, J.E., Farwell, M.A. Cytogenet. Cell Genet. (1998) [Pubmed]
Nuclear respiratory factor 2 activates transcription of human mitochondrial translation initiation factor 2 gene. Hayashi, R., Ueda, T., Farwell, M.A., Takeuchi, N. Mitochondrion (2007) [Pubmed]
The human mitochondrial translation initiation factor 2 gene (MTIF2): transcriptional analysis and identification of a pseudogene. Overman, R.G., Enderle, P.J., Farrow, J.M., Wiley, J.E., Farwell, M.A. Biochim. Biophys. Acta (2003) [Pubmed]

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AgaP_AGAP001670	Anopheles gambiae str. PEST
AT4G11160	Arabidopsis thaliana
MTIF2	Bos taurus
F46B6.6	Caenorhabditis elegans
MTIF2	Canis lupus familiaris
mtif2	Danio rerio
CG12413	Drosophila melanogaster
MTIF2	Gallus gallus
MTIF2	Macaca mulatta
MGG_11482	Magnaporthe oryzae 70-15
Mtif2	Mus musculus
Os09g0515500	Oryza sativa Japonica Group
MTIF2	Pan troglodytes
Mtif2	Rattus norvegicus
mtif2	Xenopus (Silurana) tropicalis

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