Disease relevance of Hex-A

• The phenotypes in myotonic dystrophy types 1 and 2 (DM1 and DM2) are similar, suggesting a shared pathophysiologic mechanism [1].

High impact information on Hex-A

• Antisera against purified Hex A and Hex C were raised in rabbits [2].
• Hexokinase coding DM1 and DM2 sequences were obtained from genomic DNA of a Drosophila melanogaster cell line by PCR amplification strategy [3].
• Amino acid sequence alignment of DM1 and DM2 shows approximately 45% homology with yeast and human hexokinases [3].
• Southern blot analysis suggests that the D. melanogaster genome contain a single copy of DM1 and DM2 sequences [3].

Anatomical context of Hex-A

• Northern analysis indicates DM1 is expressed as more than one transcript in adult as well as in the D.Mel2 cell line [3].

Analytical, diagnostic and therapeutic context of Hex-A

• Expression levels for DM1 and DM2 encoded message were found to be similar in different stages of development as seen by RT-PCR [3].
• Major forms of hexokinases (Hex A and Hex C) in Drosophila melanogaster were purified to homogeneity by utilizing affinity chromatography and preparative isoelectric focusing column as the key steps [2].

References