Disease relevance of TFDP3

• Intracellular flow cytometry and enzyme-linked immunospot assay showed that these CTLs were able to target a hepatoma cell line, HepG2, which is HLA-A2 and HCA661 positive [1].
• We constructed a recombinant adenovirus expressing the full-length cDNA of HCA661 gene and then transduced immature DCs, which had been generated with GM-CSF and IL-4 from peripheral blood mononuclear cell of HLA-A2(+) healthy donors [1].

High impact information on TFDP3

• Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties [2].
• Like the recently identified E2F-like proteins of Arabidopsis, E2F7 has two DNA binding domains and binds to the E2F DNA binding consensus site independently of DP co-factors [3].
• Recently, several laboratories have isolated a cDNA clone that encodes an E2F-like protein, known as E2F-1 [4].
• Using low-stringency hybridization, we have isolated cDNA clones that encode two additional E2F-like proteins, called E2F-2 and E2F-3 [4].
• Fine mapping further revealed four amino acids in this region, which were critical for the functional conversion from activation by TFDP1 to suppression by TFDP3 [5].

Biological context of TFDP3

• Consistent with this observation, we found that ectopic expression of TFDP3 impaired cell cycle progression from G(1) to S phase instead of facilitating such a transition as TFDP1 does [5].
• In contrast to the stimulatory effect of TFDP1, TFDP3 inhibited E2F-mediated transcriptional activation [5].
• Although TFDP3 retained the capacity to bind to E2F proteins, the resulting heterodimers failed to interact with the E2F consensus sequence [5].
• Here we report that MT2 includes an E2F-like binding site (GTTCGCGGGCAAA), as shown by the following evidence [6].
• The 11-bp repeats together with an E2F-like sequence, located adjacent to the major transcription initiation site, were important for the stimulation [7].

Anatomical context of TFDP3

• Two such proteins, HCA587 and HCA661, were predominantly detected in testis, but not in other normal tissues, except for a weak expression in normal pancreas [8].
• After maturation, the transduced DCs transcribed HCA661 mRNA and were able to prime the naïve T cells to become cytotoxic T lymphocytes (CTLs) [1].
• Transduction of dendritic cells with recombinant adenovirus encoding HCA661 activates autologous cytotoxic T lymphocytes to target hepatoma cells [1].

Other interactions of TFDP3

• Human TFDP3, a Novel DP Protein, Inhibits DNA Binding and Transactivation by E2F [5].
• Characterization of E2F8, a novel E2F-like cell-cycle regulated repressor of E2F-activated transcription [9].

References