Disease relevance of Skap2

• In vivo, the loss of SKAP-HOM results in a less severe clinical course of experimental autoimmune encephalomyelitis following immunization of mice with the encephalitogenic peptide of MOG (myelin oligodendrocyte glycoprotein) [1].
• For overexpression studies, mSKAP55R was cloned into a bicistronic murine stem cell virus-based retrovirus [2].

High impact information on Skap2

• Previously, several groups have provided experimental evidence that SKAP-HOM (most likely in cooperation with the cytosolic adaptor protein ADAP) is involved in regulating leukocyte adhesion [1].
• To further assess the physiological role of SKAP-HOM, we investigated the immune system of SKAP-HOM-deficient mice [1].
• Our data show that T-cell responses towards a variety of stimuli are unaffected in the absence of SKAP-HOM [1].
• In contrast, a tyrosine 260 mutant mSKAP55R had no effect on in vitro growth [2].
• Phosphorylation of mSKAP55R was examined by transient transfection of COS cells [2].

Biological context of Skap2

• In addition, we obtained evidence that SKAP-HOM is tyrosine phosphorylated in response to macrophage cell adhesion and that it forms a signalling complex with Fyb [3].
• We conclude that although Saps were produced, individual Saps were not indispensable factors for virulence during gastrointestinal infection of mice [4].

Anatomical context of Skap2

• In summary, our data suggest that SKAP-HOM is required for proper activation of the immune system, likely by regulating the cross-talk between immunoreceptors and integrins [1].
• Induced myeloid differentiation of M1 and HL-60 cell lines was associated with an eight-fold increase in mSKAP55R mRNA [2].
• Enforced expression of mSKAP55R inhibited in vitro growth of the myeloid FDC-P1 cell line and primary hematopoietic progenitors [2].
• Individual acid aspartic proteinases (Saps) 1-6 of Candida albicans are not essential for invasion and colonization of the gastrointestinal tract in mice [4].
• Furthermore, Vac1p affects chlamydospore formation, adherence to human vaginal epithelial cells, and the secretion of aspartyl proteinases (Saps) [5].

References