Disease relevance of ING3

• We previously showed two members of the ING family, ING1 and ING3 as a tumor suppressor gene in head and neck cancer [1].
• ING3 Promotes UV-induced Apoptosis via Fas/Caspase-8 Pathway in Melanoma Cells [2].
• Our data showed that nuclear ING2 expression was significantly reduced in radial growth phase (RGP), vertical growth phase (VGP), and metastatic melanomas compared with dysplastic nevi (P < 0.05) [3].
• Nuclear ING3 expression was remarkably reduced in malignant melanomas compared with dysplastic nevi (P<0.001), which was significantly correlated with the increased ING3 level in cytoplasm (P<0.05) [4].

High impact information on ING3

• ING3, a member of the ING family of growth regulators, links NuA4 to p53 function which we confirmed in vivo [5].
• Because ING1b and ING2 have been shown to be involved in cellular stress responses such as nucleotide excision repair and apoptosis after UV irradiation, we investigated whether ING3 also mediated UV-induced apoptosis [2].
• Moreover, ING3-mediated apoptosis was blocked by inhibition of caspase-8 or Fas activation [2].
• Using the stable clones of melanoma cells overexpressing ING3, we showed that overexpression of ING3 significantly promoted UV-induced apoptosis [2].
• A novel PHD-finger motif protein, p47ING3, modulates p53-mediated transcription, cell cycle control, and apoptosis [6].

Biological context of ING3

• Allelic loss and reduced expression of the ING3, a candidate tumor suppressor gene at 7q31, in human head and neck cancers [7].
• As only one missense mutation of the ING3 gene was found in HNSCC, we examined the expression level [7].
• Thus, we have isolated a novel ING family gene, p47ING3, which modulates p53-mediated transcription, cell cycle control, and apoptosis [6].
• The p47ING3 gene is located at chromosome 7q31.3 and consists of 12 exons that encode 418 amino acids [6].
• ING-1 mediates potent ADCC, but ING-2 had undetectable or very weak ADCC activity [8].

Anatomical context of ING3

• These antibodies bind to different tumor-associated antigens which are present on human breast carcinoma cell lines at 10(5)-10(6) antigens/cell; ING-1 binds to a 40-kD membrane glycoprotein, while ING-2 binds to a glycoprotein of greater than 300 kD [8].

Associations of ING3 with chemical compounds

• In contrast to ING2, ING4 is not a phosphoinositide receptor and binds with similar affinity to the different methylation states of histone-3 at lysine 4 [9].
• The family of proteins inhibitors of growth (ING) contains a PHD finger that bind to histone-3 trimethylated at lysine 4, and those of ING1 and ING2 also act as nuclear phosphoinositide receptors [9].

Other interactions of ING3

• ING3 protein harbors the PHD domain highly homologous among ING family proteins, in which we previously found mutations in a related gene, ING1 [7].

Analytical, diagnostic and therapeutic context of ING3

• Here, we used tissue microarray technology and immunohistochemistry to examine ING2 expression in human nevi and melanoma biopsies [3].

References