High impact information on P2ry4

• Thus, by the process of elimination, our data suggest that P2Y purinoceptors (P2Y2 or P2Y4) are involved in the Ca(2+)(i) response to fluid flow [1].
• Loss of nucleotide regulation of epithelial chloride transport in the jejunum of P2Y4-null mice [2].
• RT-PCR analysis of isolated colonic crypts revealed P2Y2, P2Y4 and P2Y6 specific transcripts [3].
• Luminal UTP-stimulated K+ secretion was completely absent in P2Y2/P2Y4 double receptor KO mice [3].
• To this end P2Y2 and P2Y4 knock-out mice were used to measure distal colonic ion transport in an Ussing chamber [3].

Biological context of P2ry4

• The P2Y2/P2Y4 ligand UTP activated sustained action potential firing in 54% of C fibers in a concentration-dependent manner [4].
• To isolate the mouse P2Y4 receptor gene, a mouse genomic library was screened with a human P2Y4 probe [5].

Anatomical context of P2ry4

• In summary, these results indicate that both the P2Y2 and P2Y4 receptors are present in the luminal membrane of mouse distal colonic mucosa, and stimulation of these receptors leads to K+ secretion [3].

Associations of P2ry4 with chemical compounds

• K+ secretion activated by luminal P2Y2 and P2Y4 receptors in mouse colon [3].
• P2Y4 receptors were not involved, since P2Y4-deficient mice displayed unaltered responses to ATP and UTP.The purinergic receptor antagonist suramin exerted surmountable antagonism for all agonists [6].
• In 1321N1 transfected cells, the mouse P2Y4 receptor was equally activated by UTP and ATP, and was antagonized by pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (PPADS) and Reactive Blue 2, and not by suramin [5].

References