High impact information on Mapk12

• Contra > ipsi genes included the neuronal high affinity glutamate transporter, 5-hydroxytryptamine receptor 1D, mitogen-activated protein kinase 12 and ubiquitin carboxy-terminal hydrolase L1 [1].
• Whereas treatment with Leptomycin B to prevent nuclear export processes promoted higher levels of p38- alpha/ beta staining in cardiac myocyte nuclei, there was no apparent change in SAPK3 localization under these conditions [2].
• By immunoblotting, we confirmed high expression of SAPK3 in fast, slow and mixed fibre types of murine skeletal muscle and observed significant expression restricted to heart, lung, thymus and testes [2].
• Dehydroepiandrosterone (100 nM) attenuated the translocation of stress-activated protein kinase 3, but not the others [3].
• Dehydroepiandrosterone antagonizes the neurotoxic effects of corticosterone and translocation of stress-activated protein kinase 3 in hippocampal primary cultures [3].

Biological context of Mapk12

• This is a new mechanism for the regulation of SAPK3 and suggests that its intracellular activity should not be solely assessed by its phosphorylation status [4].
• Rat ERK6, also known as SAPK3 and p38gamma, exhibits a distinct pattern of expression during muscle development in vitro and in vivo [5].
• With the exception of p38gamma/ERK6, the concentration of each signal transduction protein was uniformly higher in one muscle than in the other in all four animals [6].

Anatomical context of Mapk12

• This latter protein is localised together with SAPK3 at the mitochondria but it is not a SAPK3 substrate [4].
• ERK6 is expressed in a developmentally regulated manner in rodent skeletal muscle [5].
• Similar results are observed in vivo where ERK6 mRNA levels increase with post-natal development in rat hindlimb mixed muscle samples [5].
• Levels of mRNA transcript and protein abundance for ERK6 are increased during the differentiation of 2 rodent myoblast cell lines in culture [5].
• Immunolocalization in cultured cardiac myocytes followed by confocal microscopy showed punctate, non-nuclear SAPK3 staining [2].

Analytical, diagnostic and therapeutic context of Mapk12

• We have generated monoclonal antibodies with specificity for SAPK3 demonstrated by immunoblot analysis, immunofluorescence studies, and cloning of SAPK3 from a rat heart cDNA expression library [2].

References