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Gene Review:

CHST8 - carbohydrate (N-acetylgalactosamine 4-0)...

Homo sapiens

Synonyms: Carbohydrate sulfotransferase 8, GALNAC-4-ST1, GALNAC4ST1, GalNAc-4-O-sulfotransferase 1, GalNAc-4-ST1, ...

Okuda, T. et al., Kang, H.G. et al., Hiraoka, N. et al., Evers, M.R. et al., Xia, G. et al., et al.

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Disease relevance of CHST8

By infecting primary mouse hepatocytes with a recombinant retrovirus carrying a temperature-sensitive simian virus 40 large T antigen gene, two mouse liver epithelial cell lines, CHST8 and CHST10-2.1, were established [1].

High impact information on CHST8

ChGn1 and Chst8 are involved in the initiation of the synthesis of chondroitin sulfate and in the 4-O-sulfation of non-reducing N-acetylgalactosamine residues, respectively [2].
The properties of D4ST-1 indicate that sulfation of the GalNAc moieties in dermatan is mediated by a distinct GalNAc-4-O-sulfotransferase and occurs following epimerization of GlcUA to IdoUA [3].
Thus, as with GalNAc-4-ST1, sequences N-terminal to the catalytic domain contribute to the specificity of GalNAc-4-ST2 toward terminal beta1,4-linked GalNAc [4].
Molecular cloning and expression of an N-acetylgalactosamine-4-O-sulfotransferase that transfers sulfate to terminal and non-terminal beta 1,4-linked N-acetylgalactosamine [4].
Molecular cloning and expression of the pituitary glycoprotein hormone N-acetylgalactosamine-4-O-sulfotransferase [5].

Biological context of CHST8

The GalNAc-4-ST1 gene is located on human chromosome 19q13.1 [5].
GalNAc-4-ST1 has putative 5'-phosphosulfonate and 3'-phosphate binding sites [5].
Identity of the amino acid sequence between GalNAc4ST and human C4ST was 30% [6].

Anatomical context of CHST8

GalNAc4ST-1 and -2 transcripts are highly expressed in the pituitary gland and trachea, respectively, and GalNAc4ST-1 and -2 transcripts are reciprocally expressed in other tissues as well [7].

Associations of CHST8 with chemical compounds

In this paper, we report cloning and characterization of GalNAc 4-sulfotransferase (GalNAc4ST), which transfers sulfate to position 4 of the nonreducing terminal GalNAc residue [6].

Other interactions of CHST8

The recombinant GalNAc4ST transferred sulfate to position 4 of GalNAc residue of p-nitrophenyl GalNAc, indicating that this sulfotransferase transfers sulfate to position 4 at the nonreducing terminal GalNAc residue [6].
GalNAc4ST-1 and -2 do not add sulfate to HNK-1 precursor glycans, chondroitin, or desulfated dermatan sulfate [7].

Analytical, diagnostic and therapeutic context of CHST8

Molecular cloning and characterization of a dermatan-specific N-acetylgalactosamine 4-O-sulfotransferase [3].

References

Conditional transformation of mouse liver epithelial cells. An in vitro model for analysis of genetic events in hepatocarcinogenesis. Lee, G.H., Ogawa, K., Drinkwater, N.R. Am. J. Pathol. (1995) [Pubmed]
Glycosylation-related gene expression in prion diseases: PrPSc accumulation in scrapie infected GT1 cells depends on beta-1,4-linked GalNAc-4-SO4 hyposulfation. Barret, A., Forestier, L., Deslys, J.P., Julien, R., Gallet, P.F. J. Biol. Chem. (2005) [Pubmed]
Molecular cloning and characterization of a dermatan-specific N-acetylgalactosamine 4-O-sulfotransferase. Evers, M.R., Xia, G., Kang, H.G., Schachner, M., Baenziger, J.U. J. Biol. Chem. (2001) [Pubmed]
Molecular cloning and expression of an N-acetylgalactosamine-4-O-sulfotransferase that transfers sulfate to terminal and non-terminal beta 1,4-linked N-acetylgalactosamine. Kang, H.G., Evers, M.R., Xia, G., Baenziger, J.U., Schachner, M. J. Biol. Chem. (2001) [Pubmed]
Molecular cloning and expression of the pituitary glycoprotein hormone N-acetylgalactosamine-4-O-sulfotransferase. Xia, G., Evers, M.R., Kang, H.G., Schachner, M., Baenziger, J.U. J. Biol. Chem. (2000) [Pubmed]
Molecular cloning and characterization of GalNAc 4-sulfotransferase expressed in human pituitary gland. Okuda, T., Mita, S., Yamauchi, S., Fukuta, M., Nakano, H., Sawada, T., Habuchi, O. J. Biol. Chem. (2000) [Pubmed]
Molecular cloning and expression of two distinct human N-acetylgalactosamine 4-O-sulfotransferases that transfer sulfate to GalNAc beta 1-->4GlcNAc beta 1-->R in both N- and O-glycans. Hiraoka, N., Misra, A., Belot, F., Hindsgaul, O., Fukuda, M. Glycobiology (2001) [Pubmed]

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