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Gene Review

UBE2D2  -  ubiquitin-conjugating enzyme E2D 2

Homo sapiens

Synonyms: E2(17)KB2, PUBC1, UBC4, UBC4/5, UBC5B, ...
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High impact information on UBE2D2

  • Reconstitution of p53-ubiquitinylation reactions from purified components: the role of human ubiquitin-conjugating enzyme UBC4 and E6-associated protein (E6AP) [1].
  • We show that suppression of UbcH5B/C inhibits p53 ubiquitination and degradation [2].
  • In MCF7 cells levels of UbcH5B/C are reduced by doxorubicin and actinomycin D [2].
  • Surprisingly, with Ubc4 as the E2 enzyme, Zn(2+) ions alone are sufficient to catalyze the ubiquitination of cyclin B1 [3].
  • Nedd-4 was determined to have a second nonoverlapping E2 binding site that recognizes the first 67 amino acids of UbcH5B but not the more C-terminal portion of this E2 [4].

Biological context of UBE2D2


Anatomical context of UBE2D2

  • In contrast to UBC4 whose removal from a testis extract abrogates much of the conjugation activity, immmunodepletion of Rat100 from the extracts had little effect [10].
  • Both DEAE unadsorbed material (fraction I) and high salt eluate (fraction II) of the reticulocyte lysate are involved cooperatively in the ubiquitination process, where the ubiquitin-conjugating enzyme UBC4 can functionally substitute for fraction I [11].

Physical interactions of UBE2D2

  • To understand the basis for this interaction, we identified several basic residues of UbcH5B important for binding to CNOT4 by mutational analysis [12].

Other interactions of UBE2D2

  • Furthermore, nuclear localization of N-terminal deletion mutant Nedd4 enabled us to investigate the interaction between Nedd4 and E2 enzyme (Ubc4 or UbcH7) in the cell [13].
  • Concomitant charge-alteration of E49 of CNOT4 and K63 of UbcH5B restored binding and re-created a functional enzyme pair, indicative of an electrostatic interaction between these residues [12].
  • Regulation of p53 by the ubiquitin-conjugating enzymes UbcH5B/C in vivo [2].
  • A ubiquitin-protein ligase-like activity, partially purified from fraction II by DEAE anion-exchange chromatography, also functions in concert with UBC4 [11].

Analytical, diagnostic and therapeutic context of UBE2D2


  1. Reconstitution of p53-ubiquitinylation reactions from purified components: the role of human ubiquitin-conjugating enzyme UBC4 and E6-associated protein (E6AP). Rolfe, M., Beer-Romero, P., Glass, S., Eckstein, J., Berdo, I., Theodoras, A., Pagano, M., Draetta, G. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  2. Regulation of p53 by the ubiquitin-conjugating enzymes UbcH5B/C in vivo. Saville, M.K., Sparks, A., Xirodimas, D.P., Wardrop, J., Stevenson, L.F., Bourdon, J.C., Woods, Y.L., Lane, D.P. J. Biol. Chem. (2004) [Pubmed]
  3. APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex. Tang, Z., Li, B., Bharadwaj, R., Zhu, H., Ozkan, E., Hakala, K., Deisenhofer, J., Yu, H. Mol. Biol. Cell (2001) [Pubmed]
  4. Subcellular localization and ubiquitin-conjugating enzyme (E2) interactions of mammalian HECT family ubiquitin protein ligases. Hatakeyama, S., Jensen, J.P., Weissman, A.M. J. Biol. Chem. (1997) [Pubmed]
  5. SCF(beta-TRCP) and phosphorylation dependent ubiquitinationof I kappa B alpha catalyzed by Ubc3 and Ubc4. Strack, P., Caligiuri, M., Pelletier, M., Boisclair, M., Theodoras, A., Beer-Romero, P., Glass, S., Parsons, T., Copeland, R.A., Auger, K.R., Benfield, P., Brizuela, L., Rolfe, M. Oncogene (2000) [Pubmed]
  6. The RING-H2-finger protein APC11 as a target of hydrogen peroxide. Chang, T.S., Jeong, W., Lee, D.Y., Cho, C.S., Rhee, S.G. Free Radic. Biol. Med. (2004) [Pubmed]
  7. Structural model of the UbcH5B/CNOT4 complex revealed by combining NMR, mutagenesis, and docking approaches. Dominguez, C., Bonvin, A.M., Winkler, G.S., van Schaik, F.M., Timmers, H.T., Boelens, R. Structure (Camb.) (2004) [Pubmed]
  8. Identification of a novel ubiquitin-conjugating enzyme involved in mitotic cyclin degradation. Yu, H., King, R.W., Peters, J.M., Kirschner, M.W. Curr. Biol. (1996) [Pubmed]
  9. EL5, a rice N-acetylchitooligosaccharide elicitor-responsive RING-H2 finger protein, is a ubiquitin ligase which functions in vitro in co-operation with an elicitor-responsive ubiquitin-conjugating enzyme, OsUBC5b. Takai, R., Matsuda, N., Nakano, A., Hasegawa, K., Akimoto, C., Shibuya, N., Minami, E. Plant J. (2002) [Pubmed]
  10. Characterization of rat100, a 300-kilodalton ubiquitin-protein ligase induced in germ cells of the rat testis and similar to the Drosophila hyperplastic discs gene. Oughtred, R., Bedard, N., Adegoke, O.A., Morales, C.R., Trasler, J., Rajapurohitam, V., Wing, S.S. Endocrinology (2002) [Pubmed]
  11. Identification of an ubiquitin-ligation system for the epidermal-growth-factor receptor--herbimycin A induces in vitro ubiquitination in rabbit-reticulocyte lysate. Mori, S., Tanaka, K., Kanaki, H., Nakao, M., Anan, T., Yokote, K., Tamura, K., Saito, Y. Eur. J. Biochem. (1997) [Pubmed]
  12. An altered-specificity ubiquitin-conjugating enzyme/ubiquitin-protein ligase pair. Winkler, G.S., Albert, T.K., Dominguez, C., Legtenberg, Y.I., Boelens, R., Timmers, H.T. J. Mol. Biol. (2004) [Pubmed]
  13. Human ubiquitin-protein ligase Nedd4: expression, subcellular localization and selective interaction with ubiquitin-conjugating enzymes. Anan, T., Nagata, Y., Koga, H., Honda, Y., Yabuki, N., Miyamoto, C., Kuwano, A., Matsuda, I., Endo, F., Saya, H., Nakao, M. Genes Cells (1998) [Pubmed]
  14. Characterization of the nucleotide sequence of a polyubiquitin gene (PUBC1) from Arabian camel, Camelus dromedarius. Al-Khedhairy, A.A. J. Biochem. Mol. Biol. (2004) [Pubmed]
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