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Gene Review

UBE2L3  -  ubiquitin-conjugating enzyme E2L 3

Homo sapiens

Synonyms: E2-F1, L-UBC, UBCE7, UBCH7, UbcH7, ...
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Disease relevance of UBE2L3

  • The protein also exhibits very strong homology to a rabbit protein, E2-F1, which mediates p53 degradation driven by papilloma virus E6 protein in vitro [1].
  • We show that E2F1 expression is low in benign and localized prostate cancer, modestly elevated in metastatic lymph nodes from hormone-na??ve patients, and significantly elevated in metastatic tissues from hormone-resistant prostate cancer patients (P = 0.0006) [2].
  • Two separate sets of primers were used that recognized the regulatory retinoblastoma (Rb) protein-binding region and the functional DNA-binding region of E2F1 [3].

High impact information on UBE2L3

  • The crystal structure of the E6AP hect domain bound to the UbcH7 ubiquitin-conjugating enzyme (E2) reveals the determinants of E2-E3 specificity and provides insights into the transfer of ubiquitin from the E2 to the E3 [4].
  • Suppression of p85Cool-1/betaPix expression restores UbcH7 raft localization and Vav ubiquitination and diminishes Cdc42 activity [5].
  • Nef-mediated lipid raft exclusion of UbcH7 inhibits Cbl activity in T cells to positively regulate signaling [5].
  • Regulation of E2F1-mediated apoptosis is essential for proper cellular growth [6].
  • Correlative evidence showed the failure of hSpry2DeltaN11 and mSpry4, both deficient in c-Cbl binding, to instigate these effects. hSpry2 interacts specifically with the c-Cbl RING finger domain and displaces UbcH7 from its binding site on the E3 ligase [7].

Biological context of UBE2L3

  • We have identified a human gene, UBE2L3, localized on Chromosome (Chr) 22q11 [8].
  • In contrast to the intronless UBE2L1 gene, the coding sequence of UBE2L3 is interrupted by three large introns [8].
  • Protein:DNA interaction studies confirmed the presence of binding sites for the transcription factors AP2 and Sp1 in the UBE2L3 minimal promoter [9].
  • We investigated the role of the ubiquitin-conjugating enzyme UBCH7 in nuclear receptor transactivation [10].
  • Human homologue of Drosophila ariadne (HHARI) is a RING-IBR-RING domain protein identified through its ability to bind the human ubiquitin-conjugating enzyme, UbcH7 [11].

Anatomical context of UBE2L3


Associations of UBE2L3 with chemical compounds

  • The ubiquitin-conjugating enzyme UBCH7 acts as a coactivator for steroid hormone receptors [10].
  • Biochemically, parkin appeared most enriched in cytosolic and postsynaptic fractions of adult rat brain, but also in purified, alpha S-rich presynaptic elements that additionally contained parkin's E2-binding partner, UbcH7 [16].

Physical interactions of UBE2L3


Co-localisations of UBE2L3

  • We now demonstrate that HHARI interacts and co-localizes with UbcH7 in mammalian cells, particularly in the perinuclear region [19].

Other interactions of UBE2L3

  • Comparison of the genomic organization of UBE2L6 with UBE2L3 demonstrates that these genes remain highly conserved at the genomic as well as at the protein level [20].
  • We recently reported the identification of a RING finger-containing protein, HHARI (human homologue of Drosophila ariadne), which binds to the human ubiquitin-conjugating enzyme UbcH7 in vitro [19].
  • Furthermore, nuclear localization of N-terminal deletion mutant Nedd4 enabled us to investigate the interaction between Nedd4 and E2 enzyme (Ubc4 or UbcH7) in the cell [21].
  • Structure of an E6AP-UbcH7 complex: insights into ubiquitination by the E2-E3 enzyme cascade [4].
  • To determine whether NKLAM functions as an E3 ligase, we performed coimmunoprecipitation binding assays with ubiquitin conjugates (Ubcs) UbcH7, UbcH8, and UbcH10 [22].

Analytical, diagnostic and therapeutic context of UBE2L3

  • 3. Using chromosome-specific vectorette PCR, we have determined the intron/exon structure of UBE2L3 [8].
  • Here we analysed by in situ hybridization the expression of mRNAs for parkin and UbcR7 (rat orthologue of human UbcH7) in the developing rat brain [23].
  • In this study, we examined the expression of E2F1 in 667 prostate tissue cores and compared it with the expression of the androgen receptor (AR), a marker of prostate epithelial differentiation, using tissue microarray analysis [2].


  1. A human ubiquitin conjugating enzyme, L-UBC, maps in the Alzheimer's disease locus on chromosome 14q24.3. Robinson, P.A., Leek, J.P., Thompson, J., Carr, I.M., Bailey, A., Moynihan, T.P., Coletta, P.L., Lench, N.J., Markham, A.F. Mamm. Genome (1995) [Pubmed]
  2. Elevated E2F1 inhibits transcription of the androgen receptor in metastatic hormone-resistant prostate cancer. Davis, J.N., Wojno, K.J., Daignault, S., Hofer, M.D., Kuefer, R., Rubin, M.A., Day, M.L. Cancer Res. (2006) [Pubmed]
  3. Signal antonymy unique to myelodysplastic marrows correlates with altered expression of E2F1. Mundle, S.D., Mativi, B.Y., Cartlidge, J.D., Dangerfield, B., Broady-Robinson, L., Li, B., Shetty, V., Venugopal, P., Gregory, S.A., Preisler, H.D., Raza, A. Br. J. Haematol. (2000) [Pubmed]
  4. Structure of an E6AP-UbcH7 complex: insights into ubiquitination by the E2-E3 enzyme cascade. Huang, L., Kinnucan, E., Wang, G., Beaudenon, S., Howley, P.M., Huibregtse, J.M., Pavletich, N.P. Science (1999) [Pubmed]
  5. Nef-mediated lipid raft exclusion of UbcH7 inhibits Cbl activity in T cells to positively regulate signaling. Simmons, A., Gangadharan, B., Hodges, A., Sharrocks, K., Prabhakar, S., García, A., Dwek, R., Zitzmann, N., McMichael, A. Immunity (2005) [Pubmed]
  6. Regulation of TopBP1 oligomerization by Akt/PKB for cell survival. Liu, K., Paik, J.C., Wang, B., Lin, F.T., Lin, W.C. EMBO J. (2006) [Pubmed]
  7. Sprouty2 attenuates epidermal growth factor receptor ubiquitylation and endocytosis, and consequently enhances Ras/ERK signalling. Wong, E.S., Fong, C.W., Lim, J., Yusoff, P., Low, B.C., Langdon, W.Y., Guy, G.R. EMBO J. (2002) [Pubmed]
  8. Characterization of a human ubiquitin-conjugating enzyme gene UBE2L3. Moynihan, T.P., Ardley, H.C., Leek, J.P., Thompson, J., Brindle, N.S., Markham, A.F., Robinson, P.A. Mamm. Genome (1996) [Pubmed]
  9. Promoter analysis of the human ubiquitin-conjugating enzyme gene family UBE2L1-4, including UBE2L3 which encodes UbcH7. Ardley, H.C., Moynihan, T.P., Markham, A.F., Robinson, P.A. Biochim. Biophys. Acta (2000) [Pubmed]
  10. The ubiquitin-conjugating enzyme UBCH7 acts as a coactivator for steroid hormone receptors. Verma, S., Ismail, A., Gao, X., Fu, G., Li, X., O'Malley, B.W., Nawaz, Z. Mol. Cell. Biol. (2004) [Pubmed]
  11. Human homologue of ariadne promotes the ubiquitylation of translation initiation factor 4E homologous protein, 4EHP. Tan, N.G., Ardley, H.C., Scott, G.B., Rose, S.A., Markham, A.F., Robinson, P.A. FEBS Lett. (2003) [Pubmed]
  12. Quantitative expression analysis of the cellular specificity of HECT-domain ubiquitin E3 ligases. Scarafia, L.E., Winter, A., Swinney, D.C. Physiol. Genomics (2000) [Pubmed]
  13. Differential expression of a novel ankyrin containing E3 ubiquitin-protein ligase, Hace1, in sporadic Wilms' tumor versus normal kidney. Anglesio, M.S., Evdokimova, V., Melnyk, N., Zhang, L., Fernandez, C.V., Grundy, P.E., Leach, S., Marra, M.A., Brooks-Wilson, A.R., Penninger, J., Sorensen, P.H. Hum. Mol. Genet. (2004) [Pubmed]
  14. Ubiquitin and malignant transformation of oral mucosa. Cheng, L.H. Head & neck. (2001) [Pubmed]
  15. Melanin accumulation accelerates melanocyte senescence by a mechanism involving p16INK4a/CDK4/pRB and E2F1. Bandyopadhyay, D., Medrano, E.E. Ann. N. Y. Acad. Sci. (2000) [Pubmed]
  16. Parkin localizes to the Lewy bodies of Parkinson disease and dementia with Lewy bodies. Schlossmacher, M.G., Frosch, M.P., Gai, W.P., Medina, M., Sharma, N., Forno, L., Ochiishi, T., Shimura, H., Sharon, R., Hattori, N., Langston, J.W., Mizuno, Y., Hyman, B.T., Selkoe, D.J., Kosik, K.S. Am. J. Pathol. (2002) [Pubmed]
  17. A novel centrosomal ring-finger protein, dorfin, mediates ubiquitin ligase activity. Niwa , J., Ishigaki, S., Doyu, M., Suzuki, T., Tanaka, K., Sobue, G. Biochem. Biophys. Res. Commun. (2001) [Pubmed]
  18. UbcH7 interacts with the glucocorticoid receptor and mediates receptor autoregulation. Garside, H., Waters, C., Berry, A., Rice, L., Ardley, H.C., White, A., Robinson, P.A., Ray, D. J. Endocrinol. (2006) [Pubmed]
  19. Features of the parkin/ariadne-like ubiquitin ligase, HHARI, that regulate its interaction with the ubiquitin-conjugating enzyme, Ubch7. Ardley, H.C., Tan, N.G., Rose, S.A., Markham, A.F., Robinson, P.A. J. Biol. Chem. (2001) [Pubmed]
  20. Genomic organization of the human ubiquitin-conjugating enzyme gene, UBE2L6 on chromosome 11q12. Ardley, H.C., Rose, S.A., Tan, N., Leek, J.P., Markham, A.F., Robinson, P.A. Cytogenet. Cell Genet. (2000) [Pubmed]
  21. Human ubiquitin-protein ligase Nedd4: expression, subcellular localization and selective interaction with ubiquitin-conjugating enzymes. Anan, T., Nagata, Y., Koga, H., Honda, Y., Yabuki, N., Miyamoto, C., Kuwano, A., Matsuda, I., Endo, F., Saya, H., Nakao, M. Genes Cells (1998) [Pubmed]
  22. NK lytic-associated molecule, involved in NK cytotoxic function, is an E3 ligase. Fortier, J.M., Kornbluth, J. J. Immunol. (2006) [Pubmed]
  23. Developmental changes in the expression of parkin and UbcR7, a parkin-interacting and ubiquitin-conjugating enzyme, in rat brain. Wang, M., Suzuki, T., Kitada, T., Asakawa, S., Minoshima, S., Shimizu, N., Tanaka, K., Mizuno, Y., Hattori, N. J. Neurochem. (2001) [Pubmed]
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