The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Links

 

Gene Review

MUS81  -  MUS81 structure-specific endonuclease subunit

Homo sapiens

Synonyms: Crossover junction endonuclease MUS81, FLJ44872, SLX3
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of MUS81

 

High impact information on MUS81

 

Biological context of MUS81

  • These findings suggest that a complex network of the checkpoint pathways that respond to DNA double-strand breaks may participate in some of the phenotypes associated with Mus81 or Eme1 deficiency [1].
  • RNA interference inhibition of Mus81 reduces mitotic recombination in human cells [5].
  • We show here that the abundance of human Mus81 peaks in S-phase and remains high in cells that have completed DNA replication and that Mus81 is a predominantly nuclear protein, with super accumulation in nucleoli [6].
  • XPF and Rad1 proteins cleave the 5' side of nucleotide excision repair bubble, while Mus81 and Hef cleave similar sites of the nicked Holliday junction, fork, or flap structure [7].
 

Regulatory relationships of MUS81

  • Furthermore, Mus81 deficiency activates the G2/M checkpoint through the ATM-Chk2 pathway and promotes DNA rereplication [1].
  • The retention of human Mus81 at regions of UV-induced damage specifically in S-phase cells suggest that the enzyme is recruited to the sites at which replication forks encounter damaged DNA [6].
 

Other interactions of MUS81

  • Using immunoaffinity purification we show that hMUS81 or hMMS4 alone have no detectable nuclease activity, but that the hMUS81.hMMS4 complex is a structure-specific nuclease that is capable of resolving fork structures [8].
  • BLM helicase facilitates Mus81 endonuclease activity in human cells [4].
  • X-ray and biochemical anatomy of an archaeal XPF/Rad1/Mus81 family nuclease: similarity between its endonuclease domain and restriction enzymes [9].
  • However, the nucleolar retention of Mus81 is not dependent on the presence of BLM or WRN, or on ongoing transcription [6].
  • Mus81 is recruited to localized regions of UV damage in S-phase cells, but not in cells that are blocked from replicating DNA or that have completed replication [6].

References

  1. Haploinsufficiency of the Mus81-Eme1 endonuclease activates the intra-S-phase and G2/M checkpoints and promotes rereplication in human cells. Hiyama, T., Katsura, M., Yoshihara, T., Ishida, M., Kinomura, A., Tonda, T., Asahara, T., Miyagawa, K. Nucleic Acids Res. (2006) [Pubmed]
  2. The structure-specific endonuclease Mus81-Eme1 promotes conversion of interstrand DNA crosslinks into double-strands breaks. Hanada, K., Budzowska, M., Modesti, M., Maas, A., Wyman, C., Essers, J., Kanaar, R. EMBO J. (2006) [Pubmed]
  3. Structure of an XPF endonuclease with and without DNA suggests a model for substrate recognition. Newman, M., Murray-Rust, J., Lally, J., Rudolf, J., Fadden, A., Knowles, P.P., White, M.F., McDonald, N.Q. EMBO J. (2005) [Pubmed]
  4. BLM helicase facilitates Mus81 endonuclease activity in human cells. Zhang, R., Sengupta, S., Yang, Q., Linke, S.P., Yanaihara, N., Bradsher, J., Blais, V., McGowan, C.H., Harris, C.C. Cancer Res. (2005) [Pubmed]
  5. RNA interference inhibition of Mus81 reduces mitotic recombination in human cells. Blais, V., Gao, H., Elwell, C.A., Boddy, M.N., Gaillard, P.H., Russell, P., McGowan, C.H. Mol. Biol. Cell (2004) [Pubmed]
  6. Mus81 endonuclease localizes to nucleoli and to regions of DNA damage in human S-phase cells. Gao, H., Chen, X.B., McGowan, C.H. Mol. Biol. Cell (2003) [Pubmed]
  7. Structural and functional analyses of an archaeal XPF/Rad1/Mus81 nuclease: asymmetric DNA binding and cleavage mechanisms. Nishino, T., Komori, K., Ishino, Y., Morikawa, K. Structure (Camb.) (2005) [Pubmed]
  8. Identification and characterization of human MUS81-MMS4 structure-specific endonuclease. Oğrünç, M., Sancar, A. J. Biol. Chem. (2003) [Pubmed]
  9. X-ray and biochemical anatomy of an archaeal XPF/Rad1/Mus81 family nuclease: similarity between its endonuclease domain and restriction enzymes. Nishino, T., Komori, K., Ishino, Y., Morikawa, K. Structure (Camb.) (2003) [Pubmed]
 
WikiGenes - Universities