High impact information on SYP61

• OSM1/SYP61: a syntaxin protein in Arabidopsis controls abscisic acid-mediated and non-abscisic acid-mediated responses to abiotic stress [1].
• The stomata of osm1 plants were insensitive to both ABA-induced closing and inhibition of opening compared with wild-type plants [1].
• Analysis of the deduced amino acid sequence of the affected gene revealed that OSM1 is related most closely to mammalian syntaxins 6 and 10, which are members of the SNARE superfamily of proteins required for vesicular/target membrane fusions [1].
• The T-DNA insertion appeared in the first exon of an open reading frame on chromosome 1 (F3M18.7, which is the same as AtSYP61) [1].
• One line, osm1 (for osmotic stress-sensitive mutant), exhibited increased sensitivity to both ionic (NaCl) and nonionic (mannitol) osmotic stress in a root-bending assay [1].

Biological context of SYP61

• Here, we use a liposome fusion assay to demonstrate that VTI12 and either SYP41 or SYP61, but not both, are required for membrane fusion [2].
• This insertion mutation cosegregated closely with the osm1 phenotype and was the only functional T-DNA in the mutant genome [1].

Anatomical context of SYP61

• Furthermore, the SYP41/SYP61-residing compartment, which was defined as the TGN, was not always located along with the Golgi apparatus, suggesting that this compartment is an independent organelle distinct from the Golgi apparatus [3].

Physical interactions of SYP61

• SYP51 and SYP61 interact with each other and with VTI12, most likely also on the TGN [4].

Other interactions of SYP61

• This indicates that SYP41 and SYP61 are likely to function in independent vesicle fusion reactions in Arabidopsis [2].

References