Disease relevance of VPS25

• Nevertheless, HIV-1 release and infectivity were not reduced by efficient small interfering RNA depletion of EAP20/ESCRT-II or CHMP6/ESCRT-III [1].
• Strikingly, when apoptosis of mutant cells is blocked, tumor-like overgrowths are formed that are capable of metastasis. vps25 encodes a component of the ESCRT-II complex, which sorts membrane proteins into multivesicular bodies during endocytic trafficking to the lysosome [2].

High impact information on VPS25

• In addition, Hippo signaling is increased in vps25 clones, and hippo mutants block apoptosis in vps25 clones [3].
• In a genetic screen for suppressors of the pro-apoptotic gene hid in Drosophila, we identified two alleles of vps25, a component of the ESCRT machinery required for protein sorting at the early endosome [3].
• Inhibition of cell death in vps25 clones causes dramatic overgrowth phenotypes [3].
• vps25 mosaics display non-autonomous cell survival and overgrowth, and autonomous apoptosis [3].
• Here we report the cloning of cDNAs encoding the EAP45 and EAP20 proteins [4].

Biological context of VPS25

• Cloning and characterization of ELL-associated proteins EAP45 and EAP20. a role for yeast EAP-like proteins in regulation of gene expression by glucose [4].
• We found evidence for five ancestral VPS25 introns, intron loss, and single instances of intron gain (a Paramecium species) and intron slippage (Theileria species) [5].
• The amino acid sequences of 119 Vps25 orthologs are aligned, compared with the known secondary structure of yeast Vps25p, and used to carry out phylogenetic analysis [5].
• Residues in two amino-terminal PPXY motifs (motif I and II), involved in dimerization of Vps25p and interaction with Vps22p and Vps36p, were closely, but not absolutely conserved [5].

Anatomical context of VPS25

• Expression of mammalian VPS25 in a wide range of tissues, and the presence in a broad range of eukaryotic species, indicates a basic role in eukaryotic cell function [5].

Associations of VPS25 with chemical compounds

• Intron positions were analyzed in VPS25 from 36 species [5].
• A highly conserved carboxy-terminal lysine was identified, which suggests Vps25 is ubiquitinated [5].

Other interactions of VPS25

• Human CHMP6, a myristoylated ESCRT-III protein, interacts directly with an ESCRT-II component EAP20 and regulates endosomal cargo sorting [6].

References