Disease relevance of ATHSP101

• The ability of AtHSP101 to protect yeast from severe heat stress strongly suggests that this HSP plays an important role in thermotolerance in higher plants [1].
• Tobamovirus infection is independent of HSP101 mRNA induction and protein expression [2].
• Heat shock protein 101 (HSP101) has been implicated in tobamovirus infections by virtue of its ability to enhance translation of mRNAs possessing the 5'Omega-leader of Tobacco mosaic virus (TMV) [2].

High impact information on ATHSP101

• The phenotype of hot1 also provides direct evidence that Hsp101, which is required for thermotolerance in bacteria and yeast, is also essential for thermotolerance in a complex eukaryote [3].
• In plants, one of the best-studied proteins from this family is cytosolic ClpB1 (At1g74310), better known in Arabidopsis as AtHsp101, which is a heat shock protein required for acclimation to high temperatures [4].
• To assess oxidative damage and alterations in the heat shock response, thiobarbituric acid reactive substances, heat shock protein 101, and small heat shock protein levels were determined [5].
• Despite the complete absence of Hsp101 protein, plant growth and development, as well as seed germination, are normal, demonstrating that Hsp101 chaperone activity is not essential in the absence of stress [6].
• Southern analysis revealed that there appears to be a single gene per haploid genome coding for HSP101 protein in rice [7].

Biological context of ATHSP101

• Rice full-length hsp101 cDNA complemented yeast mutant disrupted for its own hsp104 gene by insertional mutagenesis, with efficacy that was comparable with Arabidopsis hsp101 cDNA [7].
• Analysis of the genome revealed 7 additional proteins that are variably homologous with At1g74310 throughout the entire amino acid sequence and significant similarities or identities in the signature sequences conserved among Hsp100 proteins [8].
• Collectively, the HSP101 ORF, the MULE 5' terminal inverted repeat (TIR), and the 1.3 kb immediately upstream of the TIR is located on chromosome IV, and we refer to it as HSP101B [9].

Anatomical context of ATHSP101

• Based on these observations, it is concluded that Hsc70 and Hsp101 are present in a complex in the plant cytosol [10].

Associations of ATHSP101 with chemical compounds

• The induction of HSP101 mRNA was also correlated with an increase in its protein levels and was independent of defense-related signaling pathways involving salicylic acid, jasmonic acid, or ethylene [2].

Other interactions of ATHSP101

• A high- and low-temperature inducible Arabidopsis thaliana HSP101 promoter located in a nonautonomous Mutator-like element [9].

Analytical, diagnostic and therapeutic context of ATHSP101

• Treatment with apyrase enhanced the co-immunoprecipitation of Hsp101 with Hsc70, while ATP had the opposite effect [10].

References