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Gene Review

STU2  -  Stu2p

Saccharomyces cerevisiae S288c

Synonyms: L2108, Protein STU2, Suppressor of tubulin 2, YLR045C
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High impact information on STU2

  • Instead, the checkpoint deficiency leads to deregulation of microtubule-associated proteins Cin8 and Stu2, which, in the absence of both chromosome cohesion and bipolar attachment of kinetochores to microtubules, induce untimely spindle elongation, causing premature chromosome separation [1].
  • Our data suggest that Stu2p regulates microtubule dynamics through two separate activities [2].
  • A recent study in budding yeast indicates that when a chromosome is transported to the minus end of a spindle microtubule, its kinetochore-bound microtubule plus end-tracking protein (+TIP) Stu2 may move to the plus end to promote rescue; i.e., to switch the depolymerizing end to a polymerizing end [3].
  • Thus, the SPB-associated Spc72-Stu2 complex plays a key role in regulating microtubule properties [4].
  • We further show that the activity of Stu2 is opposed by the activity of the kinesin-related protein Kip3 [5].

Biological context of STU2

  • STU2 is an essential gene and encodes a protein whose sequence is similar to proteins identified in a variety of organisms [6].
  • Interestingly, among these candidates, we identified two genes, SSO1 and STU2, involved in protein transport and spindle pole body formation, respectively, suggesting puzzling connections with the translation termination process [7].
  • Two-hybrid analysis suggests that this phosphorylation may attenuate the interaction between Kar9p and the XMAP215-homologue Stu2p [8].
  • Stu2 promotes mitotic spindle elongation in anaphase [5].
  • Control of microtubule dynamics by Stu2p is essential for spindle orientation and metaphase chromosome alignment in yeast [9].

Associations of STU2 with chemical compounds

  • We propose that phosphorylation at serine 197 regulates the release of Kar9p from Stu2p at the SPB, either to clear it from the mother-SPB or to allow it to travel to the plus end [8].

Physical interactions of STU2


Other interactions of STU2

  • Two-hybrid, coimmunoprecipitation, and in vitro binding assays demonstrate that they associate in all pairwise combinations, although the interaction between Stu2 and Bim1 may be indirect [11].
  • Thus, Stu2, Bik1, and Bim1 interact physically but do not appear to be arranged in a single unique complex [11].
  • Using live cell imaging and mutations affecting microtubule binding proteins and kinetochore function, we identify a regulatory mechanism for spindle microtubule dynamics involving Stu2p and the core kinetochore component, Ndc10p [12].


  1. DNA replication checkpoint prevents precocious chromosome segregation by regulating spindle behavior. Krishnan, V., Nirantar, S., Crasta, K., Cheng, A.Y., Surana, U. Mol. Cell (2004) [Pubmed]
  2. Stu2p binds tubulin and undergoes an open-to-closed conformational change. Al-Bassam, J., van Breugel, M., Harrison, S.C., Hyman, A. J. Cell Biol. (2006) [Pubmed]
  3. A +TIP for a smooth trip. Xiang, X. J. Cell Biol. (2006) [Pubmed]
  4. The XMAP215 homologue Stu2 at yeast spindle pole bodies regulates microtubule dynamics and anchorage. Usui, T., Maekawa, H., Pereira, G., Schiebel, E. EMBO J. (2003) [Pubmed]
  5. Stu2 promotes mitotic spindle elongation in anaphase. Severin, F., Habermann, B., Huffaker, T., Hyman, T. J. Cell Biol. (2001) [Pubmed]
  6. Stu2p: A microtubule-binding protein that is an essential component of the yeast spindle pole body. Wang, P.J., Huffaker, T.C. J. Cell Biol. (1997) [Pubmed]
  7. Gene overexpression as a tool for identifying new trans-acting factors involved in translation termination in Saccharomyces cerevisiae. Namy, O., Hatin, I., Stahl, G., Liu, H., Barnay, S., Bidou, L., Rousset, J.P. Genetics (2002) [Pubmed]
  8. The Cyclin-dependent Kinase Cdc28p Regulates Multiple Aspects of Kar9p Function in Yeast. Moore, J.K., Miller, R.K. Mol. Biol. Cell (2007) [Pubmed]
  9. Control of microtubule dynamics by Stu2p is essential for spindle orientation and metaphase chromosome alignment in yeast. Kosco, K.A., Pearson, C.G., Maddox, P.S., Wang, P.J., Adams, I.R., Salmon, E.D., Bloom, K., Huffaker, T.C. Mol. Biol. Cell (2001) [Pubmed]
  10. The yeast spindle pole body component Spc72p interacts with Stu2p and is required for proper microtubule assembly. Chen, X.P., Yin, H., Huffaker, T.C. J. Cell Biol. (1998) [Pubmed]
  11. The regulation of microtubule dynamics in Saccharomyces cerevisiae by three interacting plus-end tracking proteins. Wolyniak, M.J., Blake-Hodek, K., Kosco, K., Hwang, E., You, L., Huffaker, T.C. Mol. Biol. Cell (2006) [Pubmed]
  12. Yeast kinetochores do not stabilize Stu2p-dependent spindle microtubule dynamics. Pearson, C.G., Maddox, P.S., Zarzar, T.R., Salmon, E.D., Bloom, K. Mol. Biol. Cell (2003) [Pubmed]
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