The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

CIN8  -  Cin8p

Saccharomyces cerevisiae S288c

Synonyms: Chromosome instability protein 8, KSL2, Kinesin-like protein CIN8, SDS15, YEL061C
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on CIN8

  • For S. cerevisiae cells, the assembly of a bipolar mitotic spindle requires the action of either Cin8p or Kip1p, gene products related to the mechanochemical enzyme kinesin [1].
  • Instead, the checkpoint deficiency leads to deregulation of microtubule-associated proteins Cin8 and Stu2, which, in the absence of both chromosome cohesion and bipolar attachment of kinetochores to microtubules, induce untimely spindle elongation, causing premature chromosome separation [2].
  • In addition, we found that yeast cell viability could be supported by as few as two microtubule-based motors: the BimC-type kinesin Cin8p, required for spindle structure, paired with either Kar3p or Kip3p, required for both spindle structure and positioning [3].
  • KIP1 and CIN8 are functionally redundant: double mutants arrested in mitosis whereas the single mutants did not [4].
  • Cin8p was observed to be a component of the mitotic spindle, colocalizing with the microtubules that lie between the poles [5].

Biological context of CIN8


Anatomical context of CIN8

  • The microtubule organizing centers of arrested cells were duplicated but unseparated, indicating that KIP1 or CIN8 is required for mitotic spindle assembly [4].
  • Since ectopic expression of proteolysis-resistant Cin8, Kip1 or Ase1 is sufficient for SPB separation even in the absence of Cdc28-Clb activity, we suggest that stabilization of these mechanical force-generating proteins is the predominant role of Cdc28-Clb in centrosome separation [10].
  • These include genes whose products act in two spindle motor pathways that overlap in function with Cin8p, the kinesin-related Kip1p pathway and the cytoplasmic dynein pathway [11].
  • We report that cin8 mutants are sensitive to the cell wall disrupting agents calcofluor white and SDS [12].

Associations of CIN8 with chemical compounds


Regulatory relationships of CIN8


Other interactions of CIN8

  • KAR3 encodes a distinct kinesin-related protein whose action antagonizes Cin8p/Kip1p function [6].
  • Furthermore, we demonstrate that double mutants, defective for SLC1 and the kinesin-related CIN8 genes are non-lethal [15].
  • Loss of function of Cin8p (a yeast kinesin-like motor protein) in the absence of either Kip1p (a motor of the same family) or Dyn1p (the dynein heavy chain) is lethal [12].
  • The kinesin-related Cin8p and cytoplasmic dynein are microtubule-associated motor proteins required for anaphase spindle elongation in the yeast Saccharomyces cerevisiae [16].


  1. Kinesin-related proteins required for structural integrity of the mitotic spindle. Saunders, W.S., Hoyt, M.A. Cell (1992) [Pubmed]
  2. DNA replication checkpoint prevents precocious chromosome segregation by regulating spindle behavior. Krishnan, V., Nirantar, S., Crasta, K., Cheng, A.Y., Surana, U. Mol. Cell (2004) [Pubmed]
  3. Novel roles for saccharomyces cerevisiae mitotic spindle motors. Cottingham, F.R., Gheber, L., Miller, D.L., Hoyt, M.A. J. Cell Biol. (1999) [Pubmed]
  4. Kinesin-related proteins required for assembly of the mitotic spindle. Roof, D.M., Meluh, P.B., Rose, M.D. J. Cell Biol. (1992) [Pubmed]
  5. Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly. Hoyt, M.A., He, L., Loo, K.K., Saunders, W.S. J. Cell Biol. (1992) [Pubmed]
  6. Loss of function of Saccharomyces cerevisiae kinesin-related CIN8 and KIP1 is suppressed by KAR3 motor domain mutations. Hoyt, M.A., He, L., Totis, L., Saunders, W.S. Genetics (1993) [Pubmed]
  7. The Saccharomyces cerevisiae kinesin-related motor Kar3p acts at preanaphase spindle poles to limit the number and length of cytoplasmic microtubules. Saunders, W., Hornack, D., Lengyel, V., Deng, C. J. Cell Biol. (1997) [Pubmed]
  8. Mitotic spindle function in Saccharomyces cerevisiae requires a balance between different types of kinesin-related motors. Saunders, W., Lengyel, V., Hoyt, M.A. Mol. Biol. Cell (1997) [Pubmed]
  9. Analysis of kinesin motor function at budding yeast kinetochores. Tytell, J.D., Sorger, P.K. J. Cell Biol. (2006) [Pubmed]
  10. Cdk1 regulates centrosome separation by restraining proteolysis of microtubule-associated proteins. Crasta, K., Huang, P., Morgan, G., Winey, M., Surana, U. EMBO J. (2006) [Pubmed]
  11. Saccharomyces cerevisiae genes required in the absence of the CIN8-encoded spindle motor act in functionally diverse mitotic pathways. Geiser, J.R., Schott, E.J., Kingsbury, T.J., Cole, N.B., Totis, L.J., Bhattacharyya, G., He, L., Hoyt, M.A. Mol. Biol. Cell (1997) [Pubmed]
  12. Mutations in the yeast kinesin-like Cin8p are alleviated by osmotic support. Korolyev, E., Steinberg-Neifach, O., Eshel, D. FEMS Microbiol. Lett. (2005) [Pubmed]
  13. Motile properties of the kinesin-related Cin8p spindle motor extracted from Saccharomyces cerevisiae cells. Gheber, L., Kuo, S.C., Hoyt, M.A. J. Biol. Chem. (1999) [Pubmed]
  14. The N-end rule pathway is required for import of histidine in yeast lacking the kinesin-like protein Cin8p. Xie, Y., Varshavsky, A. Curr. Genet. (1999) [Pubmed]
  15. Molecular and genetic characterization of SLC1, a putative Saccharomyces cerevisiae homolog of the metazoan cytoplasmic dynein light chain 1. Dick, T., Surana, U., Chia, W. Mol. Gen. Genet. (1996) [Pubmed]
  16. Simultaneous expression of both MAT loci in haploid cells suppresses mutations in yeast microtubule motor genes. Steinberg-Neifach, O., Eshel, D. Mol. Gen. Genet. (2000) [Pubmed]
WikiGenes - Universities