Disease relevance of CBS2

• CBS2 protein, expressed in Escherichia coli and prepared from inclusion bodies, was used as an antigen to raise a polyclonal rabbit antiserum [1].

High impact information on CBS2

• In this paper we characterize a nuclear gene, CBP7, already known to be required for the translation of the cytochrome b transcript; we present further evidence that it is also required as a co-factor in conjunction with the maturases for the efficient processing of their intervening sequences [2].
• The AMP binding site of the gamma(1) CBS1/CBS2 pair, modeled on the structures of the CBS sequences present in the inosine monophosphate dehydrogenase crystal structure, contains three arginine residues 70, 152, and 171 and His151 [3].
• The resulting predicted functions include three particularly interesting cases of translation-associated proteins: peptidyl-tRNA hydrolase, a ribosome recycling factor homologue, and a protein similar to cytochrome b translational activator CBS2 [4].
• Recently, we provided evidence for an interaction of Cbs2p with mitochondrial ribosomes [5].
• The yeast translational activator protein Cbs2p is imported into mitochondria without obvious proteolytic processing [6].

Biological context of CBS2

• The results exclude the possibility that suppressor genes have been cloned and confirm the conclusion that both genes, CBS1 and CBS2, specifically are involved in translation of mitochondrial COB RNA [7].
• In this report, we show that CBS2 is located on chromosome IV and provide genetic evidence that the CBS2 gene encodes a polypeptide [8].
• The CBS2 gene is transcribed into low abundance mRNA species with a major transcription initiation site located 97 bp upstream from the ATG start codon next to a poly(dA-dT) stretch [8].
• Here we report on the identification of two unique chromosomal DNA-sequences of 2 kb and 2.3 kb from yeast wild type gene banks which functionally complement cbs1 and cbs2 mutants, respectively [7].
• The protein is overexpressed in mitochondrial extracts of a transformant carrying the CBS2 gene on a high copy number plasmid, but undetectable in the post-mitochondrial supernatant [1].

Anatomical context of CBS2

• Intramitochondrial localization of CBS2 was verified by in vitro import of CBS2 protein that had been synthesized in a reticulocyte lysate programmed with CBS2 mRNA transcribed in vitro [1].

Other interactions of CBS2

• In this article I review available data on CBS1p and CBS2p from the initial detection of the genes up to the current investigations on interacting components and the proteins' topology [9].
• The yeast nuclear genes CBS1 and CBS2 are both required for translation of the mitochondrial COB transcripts [7].
• So far no homologs have been identified, except for the product of the S. cerevisiae orf YHR063C, which has some similarity to Cbs2p [10].

Analytical, diagnostic and therapeutic context of CBS2

• Molecular cloning of the yeast nuclear genes CBS1 and CBS2 [7].
• Here, we demonstrate by the use of affinity chromatography and coimmunoprecipitation that Cbs2p interacts with the mitoribosomes [11].

References