The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

CDC40  -  Cdc40p

Saccharomyces cerevisiae S288c

Synonyms: Cell division control protein 40, D9481.11, PRP17, Pre-mRNA-processing factor 17, SLT15, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on CDC40

  • Furthermore, the absence of a functional Xrs2p complex leads to sensitivity to deoxynucleotide depletion and to an inability to efficiently slow down cell cycle progression in response to hydroxyurea [1].
  • This suggests that SLU7 is involved in the process of 3' splice site choice, whereas SLU4 fulfills a generic requirement for the second step [2].
  • Here, we show that MRX (Mre11-Rad50-Xrs2), an evolutionarily conserved protein complex involved in DNA double-strand break (DSB) repair, is recruited to the telomeres in late S phase [3].
  • Although SLU7 encodes an essential gene product, we find that a null allele of prp17 is temperature-sensitive for growth and has a partial splicing defect in vitro [4].
  • While most of these genes are essential for yeast mating-type (MAT) gene switching, neither RAD50 nor XRS2 is required to complete this specialized mitotic gene conversion process [5].

Biological context of CDC40


Anatomical context of CDC40


Associations of CDC40 with chemical compounds

  • RAD6 mRNA levels increase when cells are treated with MMS, but this increase seems to be due to the arrest of the cells by MMS at the repair-specific stage; cells arrested at the same stage by HU or by the cdc40 lesion also show high levels of RAD6 mRNA [10].
  • This stage is the one at which rad6 mutants arrest, as do wild-type cells exposed to hydroxyurea (HU) or methyl methanesulfonate (MMS), or cdc40 cells exposed to the restrictive temperature [10].
  • We screened a cDNA overexpression library and isolated cDNAs that specifically suppress the HU/MMS-sensitivity of cdc40 mutants [11].

Physical interactions of CDC40

  • We therefore suggest that the functional region of Prp17p that interacts with Prp18p, Prp16p, and U5 snRNA is the N terminal region of the protein [12].

Regulatory relationships of CDC40


Other interactions of CDC40

  • Moreover, other PRP8 alleles exhibit synthetic lethality with the absence of Prp17p and show a reduced ability to splice an intron bearing an altered 3' splice junction [6].
  • Interestingly, deletion of SKY1 was synthetically lethal with all prp17 mutants tested, but only with specific prp8 alleles in a domain implicated in governing fidelity of 3'AG recognition [14].
  • Genomic replacement of an intronless TUB1 gene relieves the benomyl sensitivity of prp17 mutants; however, they remain temperature sensitive, implying multiple limiting factors for mitosis [7].
  • In vitro splicing with TUB3 pre-mRNA demonstrates a compromised second step in prp17::LEU2 extracts, implicating a direct role for Prp17 in its efficient splicing [7].
  • Only mutations in the N-terminal nonconserved domain of PRP17 are synthetically lethal in combination with mutations in PRP16 and PRP18, two other gene products required for the second splicing reaction [12].

Analytical, diagnostic and therapeutic context of CDC40


  1. The yeast Xrs2 complex functions in S phase checkpoint regulation. D'Amours, D., Jackson, S.P. Genes Dev. (2001) [Pubmed]
  2. An essential splicing factor, SLU7, mediates 3' splice site choice in yeast. Frank, D., Guthrie, C. Genes Dev. (1992) [Pubmed]
  3. Late S phase-specific recruitment of Mre11 complex triggers hierarchical assembly of telomere replication proteins in Saccharomyces cerevisiae. Takata, H., Tanaka, Y., Matsuura, A. Mol. Cell (2005) [Pubmed]
  4. Characterization and functional ordering of Slu7p and Prp17p during the second step of pre-mRNA splicing in yeast. Jones, M.H., Frank, D.N., Guthrie, C. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  5. Mutations in XRS2 and RAD50 delay but do not prevent mating-type switching in Saccharomyces cerevisiae. Ivanov, E.L., Sugawara, N., White, C.I., Fabre, F., Haber, J.E. Mol. Cell. Biol. (1994) [Pubmed]
  6. Extensive genetic interactions between PRP8 and PRP17/CDC40, two yeast genes involved in pre-mRNA splicing and cell cycle progression. Ben-Yehuda, S., Russell, C.S., Dix, I., Beggs, J.D., Kupiec, M. Genetics (2000) [Pubmed]
  7. Dependence of pre-mRNA introns on PRP17, a non-essential splicing factor: implications for efficient progression through cell cycle transitions. Chawla, G., Sapra, A.K., Surana, U., Vijayraghavan, U. Nucleic Acids Res. (2003) [Pubmed]
  8. Genetic and physical interactions between factors involved in both cell cycle progression and pre-mRNA splicing in Saccharomyces cerevisiae. Ben-Yehuda, S., Dix, I., Russell, C.S., McGarvey, M., Beggs, J.D., Kupiec, M. Genetics (2000) [Pubmed]
  9. Efficient initiation of S-phase in yeast requires Cdc40p, a protein involved in pre-mRNA splicing. Boger-Nadjar, E., Vaisman, N., Ben-Yehuda, S., Kassir, Y., Kupiec, M. Mol. Gen. Genet. (1998) [Pubmed]
  10. Regulation of the RAD6 gene of Saccharomyces cerevisiae in the mitotic cell cycle and in meiosis. Kupiec, M., Simchen, G. Mol. Gen. Genet. (1986) [Pubmed]
  11. A role for the yeast cell cycle/splicing factor Cdc40 in the G(1)/S transition. Kaplan, Y., Kupiec, M. Curr. Genet. (2007) [Pubmed]
  12. Genetic studies of the PRP17 gene of Saccharomyces cerevisiae: a domain essential for function maps to a nonconserved region of the protein. Seshadri, V., Vaidya, V.C., Vijayraghavan, U. Genetics (1996) [Pubmed]
  13. The Saccharomyces cerevisiae gene CDC40/PRP17 controls cell cycle progression through splicing of the ANC1 gene. Dahan, O., Kupiec, M. Nucleic Acids Res. (2004) [Pubmed]
  14. Evidence for a role of Sky1p-mediated phosphorylation in 3' splice site recognition involving both Prp8 and Prp17/Slu4. Dagher, S.F., Fu, X.D. RNA (2001) [Pubmed]
  15. Genomewide analysis of mRNA processing in yeast using splicing-specific microarrays. Clark, T.A., Sugnet, C.W., Ares, M. Science (2002) [Pubmed]
WikiGenes - Universities