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Gene Review

TUB3  -  alpha-tubulin TUB3

Saccharomyces cerevisiae S288c

Synonyms: Tubulin alpha-3 chain, YM7056.02C, YML124C
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Disease relevance of TUB3


High impact information on TUB3

  • This defect is seen not only in 3' splice site cis-competitions but also in the splicing of an unusual intron in the TUB3 gene and in the ACT1 intron when utilization of its 3' splice site is rate limiting for splicing [2].
  • Addition of the microtubule polymerization inhibitors nocodazol or benomyl to the medium or deletion of the nonessential alpha-tubulin TUB3 gene can mostly correct the abnormal microtubule arrays and other growth defects of kar3 mutants, suggesting that these phenotypes result from excessive microtubule polymerization [3].
  • In Saccharomyces cerevisiae, this gene is distal to the TUB3 locus on the left arm of chromosome XIII and is named GTR1 [4].
  • By comparison of nucleotide sequences and by tetrad analysis with GAL80 as a standard, the PHO84 locus was mapped at a site beside the TUB3 locus on the left arm of chromosome XIII [5].
  • The yeast Saccharomyces cerevisiae has two alpha-tubulin genes, TUB1 and TUB3, either of which alone is sufficient for these processes when present in a high enough copy number [6].

Biological context of TUB3


Anatomical context of TUB3


Other interactions of TUB3

  • Through further application of this method, new mutations in TUB2 and TUB3 were isolated as unlinked noncomplementers of tub1-1 [7].
  • Tubulin was purified from wild-type and deletion strains lacking either Tub1 or Tub3, and parameters of microtubule dynamics were examined [10].
  • In vitro splicing with TUB3 pre-mRNA demonstrates a compromised second step in prp17::LEU2 extracts, implicating a direct role for Prp17 in its efficient splicing [11].
  • Surprisingly, factors involved in microtubule-mediated intracellular movement (Tub3, Dhc1, and Mlp2) appear to play no appreciable role in homolog juxtaposition, unlike their counterparts in fission yeast [12].
  • GIS5-7 are identical to the previously described genes PDE2, SGE1 and TUB3, respectively [13].


  1. Genetically essential and nonessential alpha-tubulin genes specify functionally interchangeable proteins. Schatz, P.J., Solomon, F., Botstein, D. Mol. Cell. Biol. (1986) [Pubmed]
  2. A novel role for a U5 snRNP protein in 3' splice site selection. Umen, J.G., Guthrie, C. Genes Dev. (1995) [Pubmed]
  3. The Saccharomyces cerevisiae kinesin-related motor Kar3p acts at preanaphase spindle poles to limit the number and length of cytoplasmic microtubules. Saunders, W., Hornack, D., Lengyel, V., Deng, C. J. Cell Biol. (1997) [Pubmed]
  4. Putative GTP-binding protein, Gtr1, associated with the function of the Pho84 inorganic phosphate transporter in Saccharomyces cerevisiae. Bun-Ya, M., Harashima, S., Oshima, Y. Mol. Cell. Biol. (1992) [Pubmed]
  5. The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter. Bun-Ya, M., Nishimura, M., Harashima, S., Oshima, Y. Mol. Cell. Biol. (1991) [Pubmed]
  6. Insertions of up to 17 amino acids into a region of alpha-tubulin do not disrupt function in vivo. Schatz, P.J., Georges, G.E., Solomon, F., Botstein, D. Mol. Cell. Biol. (1987) [Pubmed]
  7. Unlinked noncomplementation: isolation of new conditional-lethal mutations in each of the tubulin genes of Saccharomyces cerevisiae. Stearns, T., Botstein, D. Genetics (1988) [Pubmed]
  8. Suppression of a conditional mutation in alpha-tubulin by overexpression of two checkpoint genes. Guénette, S., Magendantz, M., Solomon, F. J. Cell. Sci. (1995) [Pubmed]
  9. Modulation of tubulin polypeptide ratios by the yeast protein Pac10p. Alvarez, P., Smith, A., Fleming, J., Solomon, F. Genetics (1998) [Pubmed]
  10. The two alpha-tubulin isotypes in budding yeast have opposing effects on microtubule dynamics in vitro. Bode, C.J., Gupta, M.L., Suprenant, K.A., Himes, R.H. EMBO Rep. (2003) [Pubmed]
  11. Dependence of pre-mRNA introns on PRP17, a non-essential splicing factor: implications for efficient progression through cell cycle transitions. Chawla, G., Sapra, A.K., Surana, U., Vijayraghavan, U. Nucleic Acids Res. (2003) [Pubmed]
  12. Analysis of close stable homolog juxtaposition during meiosis in mutants of Saccharomyces cerevisiae. Lui, D.Y., Peoples-Holst, T.L., Mell, J.C., Wu, H.Y., Dean, E.W., Burgess, S.M. Genetics (2006) [Pubmed]
  13. Yeast genes GIS1-4: multicopy suppressors of the Gal- phenotype of snf1 mig1 srb8/10/11 cells. Balciunas, D., Ronne, H. Mol. Gen. Genet. (1999) [Pubmed]
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