The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

MRE11  -  Mre11p

Saccharomyces cerevisiae S288c

Synonyms: Double-strand break repair protein MRE11, NGS1, RAD58, XRS4, YM9959.06C, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of MRE11

  • Structural and functional similarities between the SbcCD proteins of Escherichia coli and the RAD50 and MRE11 (RAD32) recombination and repair proteins of yeast [1].

High impact information on MRE11

  • The Mre11 nuclease and the ATM-related Tel1 kinase are the first proteins detected at DSBs [2].
  • The cellular response to DSBs and DNA replication stress is likely directed by the Mre11 complex detecting and processing DNA ends in conjunction with Sae2 and by RP-A recognizing single-stranded DNA and recruiting additional checkpoint and repair proteins [2].
  • Unlike the response to DSBs, Mre11 and recombination proteins are not recruited to hydroxyurea-stalled replication forks unless the forks collapse [2].
  • Mre11 forms a complex with Rad50 and Xrs2, acting as the binding core, and participates in DSB processing [3].
  • Mre11 repairs methyl methanesulfonate-induced DSBs by reactions that require the nuclease activities and those that do not [3].

Biological context of MRE11


Anatomical context of MRE11

  • Conditionally, MRE11 null chicken DT40 cells accumulate chromosome breaks and die upon Mre11 repression, showing frequent centrosome amplification [8].
  • The MRE11 gene has previously been implicated in DSB repair in the yeast Saccharomyces cerevisiae . Here we have developed a methodology to study the roles of the murine Mre11 homolog in pluripotent embryonic stem cells [9].

Associations of MRE11 with chemical compounds


Physical interactions of MRE11

  • Using a two-hybrid system, we found that Mre11 interacts with Rad50 and itself in vivo [6].
  • Requirement of the Mre11 complex and exonuclease 1 for activation of the Mec1 signaling pathway [14].
  • However, Ddc1 association with DSBs does not require the function of the Mre11 complex and Exo1 [14].
  • However, domains in Xrs2p located both 49 amino acids upstream and 104 amino acids downstream of the Mre11p binding site are required for meiotic recombination and telomere elongation, respectively, in addition to the 32-amino acid region [15].

Regulatory relationships of MRE11


Other interactions of MRE11

  • The type 1 mutations (mre2 and mre11) confer a reduction in MNase sensitivity relative to the wild-type level [19].
  • This suggests that other nucleases can compensate for loss of the Exo1 and Mre11 nucleases, but not of the Mre11-Rad50-Xrs2 complex [5].
  • RAD58 (XRS4)--a new gene in the RAD52 epistasis group [20].
  • The mre2 and mre11 deletion mutants are proficient in mitotic recombination, but are defective in meiotic recombination and in formation of viable spores [21].
  • The mre11-H125N mutation, which eliminates the nuclease activities of Mre11, causes an accumulation of unprocessed double-strand breaks (DSBs) in meiosis, but no defect in processing HO-induced DSBs in mitotic cells, suggesting the existence of redundant activities [5].

Analytical, diagnostic and therapeutic context of MRE11


  1. Structural and functional similarities between the SbcCD proteins of Escherichia coli and the RAD50 and MRE11 (RAD32) recombination and repair proteins of yeast. Sharples, G.J., Leach, D.R. Mol. Microbiol. (1995) [Pubmed]
  2. Choreography of the DNA damage response: spatiotemporal relationships among checkpoint and repair proteins. Lisby, M., Barlow, J.H., Burgess, R.C., Rothstein, R. Cell (2004) [Pubmed]
  3. Complex formation and functional versatility of Mre11 of budding yeast in recombination. Usui, T., Ohta, T., Oshiumi, H., Tomizawa, J., Ogawa, H., Ogawa, T. Cell (1998) [Pubmed]
  4. Recombination-induced CAG trinucleotide repeat expansions in yeast involve the MRE11-RAD50-XRS2 complex. Richard, G.F., Goellner, G.M., McMurray, C.T., Haber, J.E. EMBO J. (2000) [Pubmed]
  5. Overlapping functions of the Saccharomyces cerevisiae Mre11, Exo1 and Rad27 nucleases in DNA metabolism. Moreau, S., Morgan, E.A., Symington, L.S. Genetics (2001) [Pubmed]
  6. Interaction of Mre11 and Rad50: two proteins required for DNA repair and meiosis-specific double-strand break formation in Saccharomyces cerevisiae. Johzuka, K., Ogawa, H. Genetics (1995) [Pubmed]
  7. Mitotic cyclins regulate telomeric recombination in telomerase-deficient yeast cells. Grandin, N., Charbonneau, M. Mol. Cell. Biol. (2003) [Pubmed]
  8. Mre11 is essential for the maintenance of chromosomal DNA in vertebrate cells. Yamaguchi-Iwai, Y., Sonoda, E., Sasaki, M.S., Morrison, C., Haraguchi, T., Hiraoka, Y., Yamashita, Y.M., Yagi, T., Takata, M., Price, C., Kakazu, N., Takeda, S. EMBO J. (1999) [Pubmed]
  9. Conditional gene targeted deletion by Cre recombinase demonstrates the requirement for the double-strand break repair Mre11 protein in murine embryonic stem cells. Xiao, Y., Weaver, D.T. Nucleic Acids Res. (1997) [Pubmed]
  10. Exo1 roles for repair of DNA double-strand breaks and meiotic crossing over in Saccharomyces cerevisiae. Tsubouchi, H., Ogawa, H. Mol. Biol. Cell (2000) [Pubmed]
  11. Identification of new genes required for meiotic recombination in Saccharomyces cerevisiae. Ajimura, M., Leem, S.H., Ogawa, H. Genetics (1993) [Pubmed]
  12. Mutations in Mre11 phosphoesterase motif I that impair Saccharomyces cerevisiae Mre11-Rad50-Xrs2 complex stability in addition to nuclease activity. Krogh, B.O., Llorente, B., Lam, A., Symington, L.S. Genetics (2005) [Pubmed]
  13. The isolation and characterization of an alkylating-agent-sensitive yeast mutant, ngs1. Nisson, P.E., Lawrence, C.W. Mutat. Res. (1986) [Pubmed]
  14. Requirement of the Mre11 complex and exonuclease 1 for activation of the Mec1 signaling pathway. Nakada, D., Hirano, Y., Sugimoto, K. Mol. Cell. Biol. (2004) [Pubmed]
  15. Xrs2p regulates Mre11p translocation to the nucleus and plays a role in telomere elongation and meiotic recombination. Tsukamoto, Y., Mitsuoka, C., Terasawa, M., Ogawa, H., Ogawa, T. Mol. Biol. Cell (2005) [Pubmed]
  16. The nuclease activity of Mre11 is required for meiosis but not for mating type switching, end joining, or telomere maintenance. Moreau, S., Ferguson, J.R., Symington, L.S. Mol. Cell. Biol. (1999) [Pubmed]
  17. A novel mre11 mutation impairs processing of double-strand breaks of DNA during both mitosis and meiosis. Tsubouchi, H., Ogawa, H. Mol. Cell. Biol. (1998) [Pubmed]
  18. DNA structure-specific nuclease activities in the Saccharomyces cerevisiae Rad50*Mre11 complex. Trujillo, K.M., Sung, P. J. Biol. Chem. (2001) [Pubmed]
  19. Mutations in the MRE11, RAD50, XRS2, and MRE2 genes alter chromatin configuration at meiotic DNA double-stranded break sites in premeiotic and meiotic cells. Ohta, K., Nicolas, A., Furuse, M., Nabetani, A., Ogawa, H., Shibata, T. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  20. RAD58 (XRS4)--a new gene in the RAD52 epistasis group. Chepurnaya, O.V., Kozhin, S.A., Peshekhonov, V.T., Korolev, V.G. Curr. Genet. (1995) [Pubmed]
  21. Functions of the yeast meiotic recombination genes, MRE11 and MRE2. Ogawa, H., Johzuka, K., Nakagawa, T., Leem, S.H., Hagihara, A.H. Adv. Biophys. (1995) [Pubmed]
  22. S. cerevisiae Tel1p and Mre11p are required for normal levels of Est1p and Est2p telomere association. Goudsouzian, L.K., Tuzon, C.T., Zakian, V.A. Mol. Cell (2006) [Pubmed]
  23. Structure of the Rad50 x Mre11 DNA repair complex from Saccharomyces cerevisiae by electron microscopy. Anderson, D.E., Trujillo, K.M., Sung, P., Erickson, H.P. J. Biol. Chem. (2001) [Pubmed]
  24. Molecular cloning and genetic characterization of the Saccharomyces cerevisiae NGS1/MRE11 gene. Chamankhah, M., Xiao, W. Curr. Genet. (1998) [Pubmed]
WikiGenes - Universities