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Gene Review

TUB1  -  alpha-tubulin TUB1

Saccharomyces cerevisiae S288c

Synonyms: Tubulin alpha-1 chain, YML085C
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High impact information on TUB1

  • Spindle microtubules were detected by expression of a protein fusion between GFP and Tub1, the major alpha tubulin [1].
  • Filaments of cyano fluorescent protein-tagged Fin1 colocalize with filaments of green fluorescent protein-tagged Tub1 only in large-budded cells [2].
  • Removing the TUB1 intron from two other splicing mutants that arrest at G(2)/M, prp17Delta and prp22-1 strains, permits nuclear division, but suppression of the cell cycle block is less efficient [3].
  • Overexpression of the major alpha-tubulin gene (TUB1) was not lethal and did not affect chromosome segregation [4].
  • The yeast Saccharomyces cerevisiae has two alpha-tubulin genes, TUB1 and TUB3, either of which alone is sufficient for these processes when present in a high enough copy number [5].

Biological context of TUB1

  • A previously uncharacterized yeast gene (YER016w) that we have named BIM1 (binding to microtubules) was obtained from a two-hybrid screen of a yeast cDNA library using as bait the entire coding sequence of TUB1 (encoding alpha-tubulin) [6].
  • The suppression is specific for the mutation ALA422VAL in TUB1, and does not affect several other mutations in TUB1 that produce the 'no microtubule' phenotype [7].
  • It also explains why the TUB1/tub1-724 heterozygotes are cold sensitive for growth and why overexpression of Rbl2p rescues that conditional lethality [8].
  • Several of the mutants arrested growth with a sufficiently uniform morphology to indicate that TUB1 has at least one specific role in the progression of the yeast cell cycle [9].
  • We have isolated 70 conditional-lethal mutations in the TUB1 alpha-tubulin gene of the yeast Saccharomyces cerevisiae using a plasmid replacement technique [9].

Other interactions of TUB1

  • Tubulin was purified from wild-type and deletion strains lacking either Tub1 or Tub3, and parameters of microtubule dynamics were examined [10].
  • The noncomplementation between tub1 and tub2 mutations is gene specific and allele specific, suggesting that the phenotype is due to an interaction at the protein level [11].
  • Genomic replacement of an intronless TUB1 gene relieves the benomyl sensitivity of prp17 mutants; however, they remain temperature sensitive, implying multiple limiting factors for mitosis [12].
  • However, the mcm19 null mutation conferred growth defects in the presence of a mutation in the TUB1 gene coding for alpha-tubulin [13].

Analytical, diagnostic and therapeutic context of TUB1

  • A systematic study of 51 tub1 alleles suggests a correlation between specific failure to interact with Bim1p in the two-hybrid assay and synthetic lethality with the bim1Delta allele [6].
  • S1 nuclease protection assays demonstrated one initiation start site and two major stop sites for the TUB1 transcripts, suggesting that variations in 3' processing generate the alpha-tubulin messages of 2.5 and 2.0 kilobases [14].


  1. Mitosis in living budding yeast: anaphase A but no metaphase plate. Straight, A.F., Marshall, W.F., Sedat, J.W., Murray, A.W. Science (1997) [Pubmed]
  2. The Saccharomyces cerevisiae Fin1 protein forms cell cycle-specific filaments between spindle pole bodies. van Hemert, M.J., Lamers, G.E., Klein, D.C., Oosterkamp, T.H., Steensma, H.Y., van Heusden, G.P. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  3. Removal of a single alpha-tubulin gene intron suppresses cell cycle arrest phenotypes of splicing factor mutations in Saccharomyces cerevisiae. Burns, C.G., Ohi, R., Mehta, S., O'Toole, E.T., Winey, M., Clark, T.A., Sugnet, C.W., Ares, M., Gould, K.L. Mol. Cell. Biol. (2002) [Pubmed]
  4. Dominant effects of tubulin overexpression in Saccharomyces cerevisiae. Burke, D., Gasdaska, P., Hartwell, L. Mol. Cell. Biol. (1989) [Pubmed]
  5. Insertions of up to 17 amino acids into a region of alpha-tubulin do not disrupt function in vivo. Schatz, P.J., Georges, G.E., Solomon, F., Botstein, D. Mol. Cell. Biol. (1987) [Pubmed]
  6. BIM1 encodes a microtubule-binding protein in yeast. Schwartz, K., Richards, K., Botstein, D. Mol. Biol. Cell (1997) [Pubmed]
  7. Suppression of a conditional mutation in alpha-tubulin by overexpression of two checkpoint genes. Guénette, S., Magendantz, M., Solomon, F. J. Cell. Sci. (1995) [Pubmed]
  8. An alpha-tubulin mutant destabilizes the heterodimer: phenotypic consequences and interactions with tubulin-binding proteins. Vega, L.R., Fleming, J., Solomon, F. Mol. Biol. Cell (1998) [Pubmed]
  9. Isolation and characterization of conditional-lethal mutations in the TUB1 alpha-tubulin gene of the yeast Saccharomyces cerevisiae. Schatz, P.J., Solomon, F., Botstein, D. Genetics (1988) [Pubmed]
  10. The two alpha-tubulin isotypes in budding yeast have opposing effects on microtubule dynamics in vitro. Bode, C.J., Gupta, M.L., Suprenant, K.A., Himes, R.H. EMBO Rep. (2003) [Pubmed]
  11. Unlinked noncomplementation: isolation of new conditional-lethal mutations in each of the tubulin genes of Saccharomyces cerevisiae. Stearns, T., Botstein, D. Genetics (1988) [Pubmed]
  12. Dependence of pre-mRNA introns on PRP17, a non-essential splicing factor: implications for efficient progression through cell cycle transitions. Chawla, G., Sapra, A.K., Surana, U., Vijayraghavan, U. Nucleic Acids Res. (2003) [Pubmed]
  13. The IML3/MCM19 gene of Saccharomyces cerevisiae is required for a kinetochore-related process during chromosome segregation. Ghosh, S.K., Poddar, A., Hajra, S., Sanyal, K., Sinha, P. Mol. Genet. Genomics (2001) [Pubmed]
  14. Expression of alpha- and beta-tubulin genes during dimorphic-phase transitions of Histoplasma capsulatum. Harris, G.S., Keath, E.J., Medoff, J. Mol. Cell. Biol. (1989) [Pubmed]
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