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Gene Review

TAF14  -  TATA-binding protein-associated factor TAF14

Saccharomyces cerevisiae S288c

Synonyms: ANC1, Actin non-complementing mutant 1, CST10, Chromosome stability protein 10, SWI/SNF chromatin-remodeling complex subunit TAF14, ...
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Disease relevance of TAF14


High impact information on TAF14

  • The yeast protein also contains a third subunit, Tfg3, which is less tightly associated and at most stimulatory to transcription, dispensable for cell viability, and has no known counterpart in mammalian TFIIF [2].
  • Importantly, we find that the luminal C-terminal domain of SUN1 interacts with the mammalian ANC-1 homologs nesprins 1 and 2 via their conserved KASH domain [3].
  • In addition to the pol II subunits, the complex was found to contain three subunits of a transcription factor TFIIF (TFIIF alpha, TFIIF beta, and Tfg3) and TFIIF-interacting CTD-phosphatase Fcp1 [4].
  • TFG3 interacted with the SNF5 component of the SWI/SNF complex in protein interaction blots [5].
  • KASH domains are conserved in the nuclear envelope proteins Syne/nesprins, Klarsicht, MSP-300, and ANC-1 [6].

Biological context of TAF14

  • Morphological abnormalities exhibited by anc1 mutants include failure to form a mating projection in response to alpha-factor and development of swollen or elongated cell shapes during proliferation [7].
  • These phenotypes demonstrate that Anc1p is important for actin function and for the functions of other proteins involved in morphogenesis [7].
  • Mutations in the ANC1 gene were shown to cause osmosensitivity and defects in actin organization; phenotypes that are similar to those caused by act1 mutations [8].
  • The Anc1 protein co-purifies with general transcription factors, chromatin remodeling complexes, and histone modification enzymes and is required for efficient transcription in yeast [9].
  • Anc1 interacts with the catalytic subunits of the general transcription factors TFIID and TFIIF, the chromatin remodeling complexes RSC and INO80, and the histone acetyltransferase complex NuA3 [9].

Anatomical context of TAF14

  • In further support of these roles for Anc1p, the anc1 delta 1::HIS3 mutation was found to be synthetically lethal in combination with a null mutation in SLA1, a gene that is important for membrane cytoskeleton function [7].

Associations of TAF14 with chemical compounds

  • We show that Anc1 is required for growth on galactose as the sole carbon source, and that it is recruited to the UAS of the GAL1 gene after induction [9].
  • The yeast mitochondrial adenine nucleotide carrier isoform encoded by the ANC2 gene has been specifically expressed in a yeast strain disrupted for the two other genes, ANC1 and ANC3 [10].

Physical interactions of TAF14

  • The interaction of recombinant Tfg3 with TATA-binding protein (TBP), the central subunit of TFIID, was temperature-dependent [11].

Regulatory relationships of TAF14


Other interactions of TAF14

  • In addition, mutant alleles of four ANC genes (ANC1, ANC2, ANC3 and ANC4) were tested for interactions with null alleles of actin-binding protein genes [13].
  • We identify ANC1 as the target of CDC40 regulation [12].
  • The 14 extragenic noncomplementing mutations represent alleles of at least four different genes, ANC1, ANC2, ANC3 and ANC4 (Actin NonComplementing) [8].


  1. The Yaf9 component of the SWR1 and NuA4 complexes is required for proper gene expression, histone H4 acetylation, and Htz1 replacement near telomeres. Zhang, H., Richardson, D.O., Roberts, D.N., Utley, R., Erdjument-Bromage, H., Tempst, P., Côté, J., Cairns, B.R. Mol. Cell. Biol. (2004) [Pubmed]
  2. TFIIF-TAF-RNA polymerase II connection. Henry, N.L., Campbell, A.M., Feaver, W.J., Poon, D., Weil, P.A., Kornberg, R.D. Genes Dev. (1994) [Pubmed]
  3. SUN1 Interacts with Nuclear Lamin A and Cytoplasmic Nesprins To Provide a Physical Connection between the Nuclear Lamina and the Cytoskeleton. Haque, F., Lloyd, D.J., Smallwood, D.T., Dent, C.L., Shanahan, C.M., Fry, A.M., Trembath, R.C., Shackleton, S. Mol. Cell. Biol. (2006) [Pubmed]
  4. Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and the Rpb4 subunit of pol II. Kimura, M., Suzuki, H., Ishihama, A. Mol. Cell. Biol. (2002) [Pubmed]
  5. TFG/TAF30/ANC1, a component of the yeast SWI/SNF complex that is similar to the leukemogenic proteins ENL and AF-9. Cairns, B.R., Henry, N.L., Kornberg, R.D. Mol. Cell. Biol. (1996) [Pubmed]
  6. UNC-83 IS a KASH protein required for nuclear migration and is recruited to the outer nuclear membrane by a physical interaction with the SUN protein UNC-84. McGee, M.D., Rillo, R., Anderson, A.S., Starr, D.A. Mol. Biol. Cell (2006) [Pubmed]
  7. A nuclear protein with sequence similarity to proteins implicated in human acute leukemias is important for cellular morphogenesis and actin cytoskeletal function in Saccharomyces cerevisiae. Welch, M.D., Drubin, D.G. Mol. Biol. Cell (1994) [Pubmed]
  8. Screens for extragenic mutations that fail to complement act1 alleles identify genes that are important for actin function in Saccharomyces cerevisiae. Welch, M.D., Vinh, D.B., Okamura, H.H., Drubin, D.G. Genetics (1993) [Pubmed]
  9. Anc1 interacts with the catalytic subunits of the general transcription factors TFIID and TFIIF, the chromatin remodeling complexes RSC and INO80, and the histone acetyltransferase complex NuA3. Kabani, M., Michot, K., Boschiero, C., Werner, M. Biochem. Biophys. Res. Commun. (2005) [Pubmed]
  10. Biochemical characterisation of the isolated Anc2 adenine nucleotide carrier from Saccharomyces cerevisiae mitochondria. Brandolin, G., Le Saux, A., Trezeguet, V., Vignais, P.V., Lauquin, G.J. Biochem. Biophys. Res. Commun. (1993) [Pubmed]
  11. Tfg3, a subunit of the general transcription factor TFIIF in Schizosaccharomyces pombe, functions under stress conditions. Kimura, M., Ishihama, A. Nucleic Acids Res. (2004) [Pubmed]
  12. The Saccharomyces cerevisiae gene CDC40/PRP17 controls cell cycle progression through splicing of the ANC1 gene. Dahan, O., Kupiec, M. Nucleic Acids Res. (2004) [Pubmed]
  13. Genetic evidence for functional interactions between actin noncomplementing (Anc) gene products and actin cytoskeletal proteins in Saccharomyces cerevisiae. Vinh, D.B., Welch, M.D., Corsi, A.K., Wertman, K.F., Drubin, D.G. Genetics (1993) [Pubmed]
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