Disease relevance of RFC5

• Upon overexpression in Escherichia coli Rfc5p has an apparent molecular mass of 41 kDa [1].

High impact information on RFC5

• We identified YDR499W as a Saccharomyces cerevisiae open reading frame with homology to several checkpoint proteins, including S. cerevisiae Rfc5p and Schizosaccharomyces pombe Rad26 [2].
• The RFC5 gene encodes a small subunit of replication factor C complex [3].
• Overexpression of POL30, which encodes the proliferating cell nuclear antigen, suppressed the replication defect of the rfc5 mutant but not its checkpoint defect [3].
• The rfc4-2 mutant itself is sensitive to hydroxyurea, and like rfc2 and rfc5 strains, it exhibits defects in the DNA replication block and intra-S checkpoints [4].
• RAD24 and RFC5 are required for DNA damage checkpoint control in the budding yeast Saccharomyces cerevisiae [5].

Biological context of RFC5

• Furthermore, the inducibility of RNR3 transcription in response to DNA damage is dependent on RFC5 [6].
• RFC5 is necessary for slowing the S-phase progression in response to DNA damage [6].
• Rfc5, a small subunit of replication factor C complex, couples DNA replication and mitosis in budding yeast [3].
• Rfc5, in cooperation with rad24, controls DNA damage checkpoints throughout the cell cycle in Saccharomyces cerevisiae [5].
• Rad24, like Rfc5, is required for the regulation of Rad53 phosphorylation in response to DNA damage [7].

Associations of RFC5 with chemical compounds

• Unlike the checkpoint-defective rfc5-1 mutation, the RFC5 suppressor mutations did not interfere with the methylmethane sulfonate- or hydroxyurea-induced phosphorylation of Rad53p [8].

Other interactions of RFC5

• Our results, together with the observation that overexpression of RAD53 suppresses the defects of the rfc5-1 mutation, suggest that Rfc5 is part of a mechanism transducing the DNA damage signal to the activation of the central transducer Rad53 [6].

References