High impact information on LCD1

• DDC2 is a novel component of the DNA integrity checkpoint pathway, which is required for proper checkpoint response to DNA damage and to incomplete DNA replication [1].
• By fusing PIE-1 sequences to the yeast GAL4 DNA-binding domain, we have identified a PIE-1 repression domain that appears to inhibit the transcriptional machinery directly [2].
• The Lcd1p/Mec1p complex is crucial for normal S phase progression and for signaling DNA damage [3].
• Hyperactivation of the yeast DNA damage checkpoint by TEL1 and DDC2 overexpression [4].
• In addition, cells lacking LCD1 are completely defective in the G(1)/S and G(2)/M DNA damage checkpoints [5].

Biological context of LCD1

• Cells lacking LCD1 are very sensitive to a range of DNA-damaging agents, including UV irradiation, and to the inhibition of DNA replication [5].
• LCD1: an essential gene involved in checkpoint control and regulation of the MEC1 signalling pathway in Saccharomyces cerevisiae [5].
• Pie1, a protein interacting with Mec1, controls cell growth and checkpoint responses in Saccharomyces cerevisiae [6].
• Mutation of the region in Pie1 homologous to Rad26 results in a phenotype similar to that of the pie1Delta mutation [6].
• Thus, Pie1 is structurally and functionally related to Rad26 and interacts with Mec1 to control checkpoints and cell proliferation [6].

Anatomical context of LCD1

• Our data suggests AIR-1 plays a role in P-granule segregation and the association of the germline factor PIE-1 with centrosomes [7].

Associations of LCD1 with chemical compounds

• Mutagenesis analysis show that YDR499Wp contains an NES that conforms to the consensus sequence for leucine-rich NESs [8].

Physical interactions of LCD1

• Finally, we reveal that endogenous Mec1p co-immunoprecipitates with Lcd1p both before and after treatment with DNA-damaging agents [5].

Other interactions of LCD1

• Pie1 has a limited homology to fission yeast Rad26, which forms a complex with the ATR family protein Rad3 [6].
• These proteins are the 5' RNA triphosphatase Ctl1p, the cell cycle-regulated transcription factor Ace2p, and a protein encoded by the previously uncharacterized open reading frame YDR499W [8].

Analytical, diagnostic and therapeutic context of LCD1

• We have determined that PIE-1 can function as a transcriptional repressor in cell culture assays [2].

References