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LCD1  -  Lcd1p

Saccharomyces cerevisiae S288c

Synonyms: DDC2, DNA damage checkpoint protein 2, DNA damage checkpoint protein LCD1, Lethal, checkpoint-defective, DNA damage-sensitive protein 1, PIE1, ...
 
 
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High impact information on LCD1

  • DDC2 is a novel component of the DNA integrity checkpoint pathway, which is required for proper checkpoint response to DNA damage and to incomplete DNA replication [1].
  • By fusing PIE-1 sequences to the yeast GAL4 DNA-binding domain, we have identified a PIE-1 repression domain that appears to inhibit the transcriptional machinery directly [2].
  • The Lcd1p/Mec1p complex is crucial for normal S phase progression and for signaling DNA damage [3].
  • Hyperactivation of the yeast DNA damage checkpoint by TEL1 and DDC2 overexpression [4].
  • In addition, cells lacking LCD1 are completely defective in the G(1)/S and G(2)/M DNA damage checkpoints [5].
 

Biological context of LCD1

 

Anatomical context of LCD1

  • Our data suggests AIR-1 plays a role in P-granule segregation and the association of the germline factor PIE-1 with centrosomes [7].
 

Associations of LCD1 with chemical compounds

 

Physical interactions of LCD1

  • Finally, we reveal that endogenous Mec1p co-immunoprecipitates with Lcd1p both before and after treatment with DNA-damaging agents [5].
 

Other interactions of LCD1

  • Pie1 has a limited homology to fission yeast Rad26, which forms a complex with the ATR family protein Rad3 [6].
  • These proteins are the 5' RNA triphosphatase Ctl1p, the cell cycle-regulated transcription factor Ace2p, and a protein encoded by the previously uncharacterized open reading frame YDR499W [8].
 

Analytical, diagnostic and therapeutic context of LCD1

  • We have determined that PIE-1 can function as a transcriptional repressor in cell culture assays [2].

References

  1. The checkpoint protein Ddc2, functionally related to S. pombe Rad26, interacts with Mec1 and is regulated by Mec1-dependent phosphorylation in budding yeast. Paciotti, V., Clerici, M., Lucchini, G., Longhese, M.P. Genes Dev. (2000) [Pubmed]
  2. Transcriptional repression by the Caenorhabditis elegans germ-line protein PIE-1. Batchelder, C., Dunn, M.A., Choy, B., Suh, Y., Cassie, C., Shim, E.Y., Shin, T.H., Mello, C., Seydoux, G., Blackwell, T.K. Genes Dev. (1999) [Pubmed]
  3. Lcd1p recruits Mec1p to DNA lesions in vitro and in vivo. Rouse, J., Jackson, S.P. Mol. Cell (2002) [Pubmed]
  4. Hyperactivation of the yeast DNA damage checkpoint by TEL1 and DDC2 overexpression. Clerici, M., Paciotti, V., Baldo, V., Romano, M., Lucchini, G., Longhese, M.P. EMBO J. (2001) [Pubmed]
  5. LCD1: an essential gene involved in checkpoint control and regulation of the MEC1 signalling pathway in Saccharomyces cerevisiae. Rouse, J., Jackson, S.P. EMBO J. (2000) [Pubmed]
  6. Pie1, a protein interacting with Mec1, controls cell growth and checkpoint responses in Saccharomyces cerevisiae. Wakayama, T., Kondo, T., Ando, S., Matsumoto, K., Sugimoto, K. Mol. Cell. Biol. (2001) [Pubmed]
  7. A highly conserved centrosomal kinase, AIR-1, is required for accurate cell cycle progression and segregation of developmental factors in Caenorhabditis elegans embryos. Schumacher, J.M., Ashcroft, N., Donovan, P.J., Golden, A. Development (1998) [Pubmed]
  8. Identification of novel Saccharomyces cerevisiae proteins with nuclear export activity: cell cycle-regulated transcription factor ace2p shows cell cycle-independent nucleocytoplasmic shuttling. Jensen, T.H., Neville, M., Rain, J.C., McCarthy, T., Legrain, P., Rosbash, M. Mol. Cell. Biol. (2000) [Pubmed]
 
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