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Gene Review

DUT1  -  bifunctional dITP/dUTP diphosphatase

Saccharomyces cerevisiae S288c

Synonyms: Deoxyuridine 5'-triphosphate nucleotidohydrolase, YBR1705, YBR252W, dUTP pyrophosphatase, dUTPase
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Disease relevance of DUT1

  • The activities of dUTPase and uracil-DNA glycosylase, key enzymes in uracil-DNA metabolism, were diminished or augmented, and the manipulated strains were analyzed for biochemical endpoints of toxicity [1].

High impact information on DUT1


Biological context of DUT1

  • The Dut1-1 protein possesses a single amino acid substitution (Gly82Ser) in a conserved motif nearby the active site and exhibits a greatly reduced dUTPase activity [3].
  • The 5' flanking sequence of DUT1 contains an MluI site typical of MCB cell-cycle-dependent UAS elements of budding and fission yeast [4].
  • However, in dut1 ung1 double mutants, starvation for dTMP causes dividing cells to arrest and die in all phases of the cell cycle [2].
  • Cells overexpressing dUTPase were protected from cytotoxicity by their ability to prevent dUTP pool expansion and were able to recover from an early S-phase checkpoint arrest [1].
  • The complete sequence of a 6794 bp segment located on the right arm of chromosome II of Saccharomyces cerevisiae. Finding of a putative dUTPase in a yeast [5].

Associations of DUT1 with chemical compounds


Regulatory relationships of DUT1

  • Our findings indicate that the relative expression levels of both dUTPase and uracil-DNA glycosylase can have great influence over the efficacy of thymidylate synthase-directed chemotherapy, thereby enhancing the candidacy of these proteins as prognostic markers and alternative targets for therapeutic development [1].


  1. dUTPase and uracil-DNA glycosylase are central modulators of antifolate toxicity in Saccharomyces cerevisiae. Tinkelenberg, B.A., Hansbury, M.J., Ladner, R.D. Cancer Res. (2002) [Pubmed]
  2. dUTP pyrophosphatase is an essential enzyme in Saccharomyces cerevisiae. Gadsden, M.H., McIntosh, E.M., Game, J.C., Wilson, P.J., Haynes, R.H. EMBO J. (1993) [Pubmed]
  3. dUTPase activity is critical to maintain genetic stability in Saccharomyces cerevisiae. Guillet, M., Van Der Kemp, P.A., Boiteux, S. Nucleic Acids Res. (2006) [Pubmed]
  4. MluI site-dependent transcriptional regulation of the Candida albicans dUTPase gene. McIntosh, E.M., Looser, J., Haynes, R.H., Pearlman, R.E. Curr. Genet. (1994) [Pubmed]
  5. The complete sequence of a 6794 bp segment located on the right arm of chromosome II of Saccharomyces cerevisiae. Finding of a putative dUTPase in a yeast. Doignon, F., Biteau, N., Aigle, M., Crouzet, M. Yeast (1993) [Pubmed]
  6. Crystallization and preliminary X-ray crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Saccharomyces cerevisiae. Han, B.W., Lee, J.Y., Yang, J.K., Lee, B.I., Suh, S.W. Acta Crystallogr. D Biol. Crystallogr. (2001) [Pubmed]
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