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Gene Review

PEX14  -  Pex14p

Saccharomyces cerevisiae S288c

Synonyms: G1870, Peroxin-14, Peroxisomal membrane protein PEX14, YGL153W
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High impact information on PEX14

  • Pex14p, an S. cerevisiae peroxin, is attached to the outer face of the peroxisomal membrane and is a component of the protein import machinery [1].
  • Pex14p, a peroxisomal membrane protein binding both receptors of the two PTS-dependent import pathways [1].
  • While the function of most small signaling domains is confined to binary ligand interactions, the peroxisomal Pex13p SH3 domain has the unique capacity of binding to two different ligands, Pex5p and Pex14p [2].
  • Involvement of Pex13p in Pex14p localization and peroxisomal targeting signal 2-dependent protein import into peroxisomes [3].
  • From these and other studies we conclude that Pex17p, in addition to Pex13p and Pex14p, is the third identified component of the peroxisomal translocation machinery [4].

Biological context of PEX14


Anatomical context of PEX14

  • Peroxisome formation from the preperoxisomal compartment depends on the activity of the genes PEX14 and PEX19, which are required for the targeting of peroxisomal matrix and membrane proteins, respectively [8].
  • Pex14p was characterized as an integral membrane protein of peroxisomes, exposing its N- and C-terminal parts to the cytosol [9].

Associations of PEX14 with chemical compounds

  • Site-directed mutagenesis of the conserved tryptophan residue within a reverse WXXXF motif abolished two-hybrid binding with the N-terminal half of Pex14p [10].
  • In cells of a pex19 disruption strain (Hppex19), induced on methanol, peroxisome structures were not detectable; peroxisomal matrix proteins accumulated in the cytosol, whereas peroxisomal membrane proteins (PMPs) were mislocalized to the cytosol (Pex3p) and mitochondria (Pex14p) or strongly reduced to undetectable levels (Pex10p) [11].
  • These organelles, which contained typical peroxisomal matrix and membrane proteins (alcohol oxidase, catalase, Pex3p, Pex10p and Pex14p), showed a relatively low density (1.18 g cm-3) after sucrose gradient centrifugation of WT::PAOX.PEX3(7x) homogenates, compared to normal peroxisomes (1.23 g cm-3) [12].

Physical interactions of PEX14

  • On the basis of these and other published data, we propose that the C terminus of Pex14p contains the actual docking site and discuss the possibility that the N terminus could be involved in a Pex5p-Pex14p association inside the peroxisomal membrane [6].

Other interactions of PEX14


  1. Pex14p, a peroxisomal membrane protein binding both receptors of the two PTS-dependent import pathways. Albertini, M., Rehling, P., Erdmann, R., Girzalsky, W., Kiel, J.A., Veenhuis, M., Kunau, W.H. Cell (1997) [Pubmed]
  2. Topography for independent binding of alpha-helical and PPII-helical ligands to a peroxisomal SH3 domain. Douangamath, A., Filipp, F.V., Klein, A.T., Barnett, P., Zou, P., Voorn-Brouwer, T., Vega, M.C., Mayans, O.M., Sattler, M., Distel, B., Wilmanns, M. Mol. Cell (2002) [Pubmed]
  3. Involvement of Pex13p in Pex14p localization and peroxisomal targeting signal 2-dependent protein import into peroxisomes. Girzalsky, W., Rehling, P., Stein, K., Kipper, J., Blank, L., Kunau, W.H., Erdmann, R. J. Cell Biol. (1999) [Pubmed]
  4. Pex17p of Saccharomyces cerevisiae is a novel peroxin and component of the peroxisomal protein translocation machinery. Huhse, B., Rehling, P., Albertini, M., Blank, L., Meller, K., Kunau, W.H. J. Cell Biol. (1998) [Pubmed]
  5. Cloning of two splice variants of the rice PTS1 receptor, OsPex5pL and OsPex5pS, and their functional characterization using pex5-deficient yeast and Arabidopsis. Lee, J.R., Jang, H.H., Park, J.H., Jung, J.H., Lee, S.S., Park, S.K., Chi, Y.H., Moon, J.C., Lee, Y.M., Kim, S.Y., Kim, J.Y., Yun, D.J., Cho, M.J., Lee, K.O., Lee, S.Y. Plant J. (2006) [Pubmed]
  6. Yeast Pex14p possesses two functionally distinct Pex5p and one Pex7p binding sites. Niederhoff, K., Meindl-Beinker, N.M., Kerssen, D., Perband, U., Schäfer, A., Schliebs, W., Kunau, W.H. J. Biol. Chem. (2005) [Pubmed]
  7. Saccharomyces cerevisiae PTS1 receptor Pex5p interacts with the SH3 domain of the peroxisomal membrane protein Pex13p in an unconventional, non-PXXP-related manner. Bottger, G., Barnett, P., Klein, A.T., Kragt, A., Tabak, H.F., Distel, B. Mol. Biol. Cell (2000) [Pubmed]
  8. Pex3p initiates the formation of a preperoxisomal compartment from a subdomain of the endoplasmic reticulum in Saccharomyces cerevisiae. Tam, Y.Y., Fagarasanu, A., Fagarasanu, M., Rachubinski, R.A. J. Biol. Chem. (2005) [Pubmed]
  9. The peroxin Pex14p. cDNA cloning by functional complementation on a Chinese hamster ovary cell mutant, characterization, and functional analysis. Shimizu, N., Itoh, R., Hirono, Y., Otera, H., Ghaedi, K., Tateishi, K., Tamura, S., Okumoto, K., Harano, T., Mukai, S., Fujiki, Y. J. Biol. Chem. (1999) [Pubmed]
  10. Membrane association of the cycling peroxisome import receptor pex5p. Kerssen, D., Hambruch, E., Klaas, W., Platta, H.W., de Kruijff, B., Erdmann, R., Kunau, W.H., Schliebs, W. J. Biol. Chem. (2006) [Pubmed]
  11. Hansenula polymorpha Pex19p is essential for the formation of functional peroxisomal membranes. Otzen, M., Perband, U., Wang, D., Baerends, R.J., Kunau, W.H., Veenhuis, M., Van der Klei, I.J. J. Biol. Chem. (2004) [Pubmed]
  12. Deviant Pex3p levels affect normal peroxisome formation in Hansenula polymorpha: a sharp increase of the protein level induces the proliferation of numerous, small protein-import competent peroxisomes. Baerends, R.J., Salomons, F.A., Kiel, J.A., van der Klei, I.J., Veenhuis, M. Yeast (1997) [Pubmed]
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