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Gene Review

PNC1  -  nicotinamidase

Saccharomyces cerevisiae S288c

Synonyms: NAMase, Nicotinamidase, Nicotine deamidase, YGL037C
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Disease relevance of PNC1


High impact information on PNC1

  • We provide evidence that nicotinamide depletion is sufficient to activate Sir2 and that this is the mechanism by which PNC1 regulates longevity [3].
  • Here we show that increased expression of PNC1 (pyrazinamidase/nicotinamidase 1), which encodes an enzyme that deaminates nicotinamide, is both necessary and sufficient for lifespan extension by calorie restriction and low-intensity stress [3].
  • Furthermore, PNC1 overexpression suppresses the inhibitory effect of exogenously added NAM on silencing, life span, and Hst1-mediated transcriptional repression [4].
  • Pnc1 is a nicotinamidase that converts NAM to nicotinic acid [4].
  • Additional copies of the salvage pathway genes, PNC1, NMA1, and NMA2, increase telomeric and rDNA silencing, implying that multiple steps affect the rate of the pathway [5].

Biological context of PNC1

  • Using a P(PNC1)::lacZ gene fusion, we have shown that this stimulation of NAMase activity results from increased levels of the protein and requires stress response elements in the 5'non-coding region of PNC1 [2].

Associations of PNC1 with chemical compounds


Regulatory relationships of PNC1


Other interactions of PNC1

  • Fewer changes were observed during the third generation but certain stress-response proteins such as Hsp26p, Ssa4p and Pnc1p exhibited constitutive expression in subsequent generations [8].


  1. The reported human NADsyn2 is ammonia-dependent NAD synthetase from a pseudomonad. Bieganowski, P., Brenner, C. J. Biol. Chem. (2003) [Pubmed]
  2. Identification and functional analysis of the Saccharomyces cerevisiae nicotinamidase gene, PNC1. Ghislain, M., Talla, E., François, J.M. Yeast (2002) [Pubmed]
  3. Nicotinamide and PNC1 govern lifespan extension by calorie restriction in Saccharomyces cerevisiae. Anderson, R.M., Bitterman, K.J., Wood, J.G., Medvedik, O., Sinclair, D.A. Nature (2003) [Pubmed]
  4. Nicotinamide clearance by Pnc1 directly regulates Sir2-mediated silencing and longevity. Gallo, C.M., Smith, D.L., Smith, J.S. Mol. Cell. Biol. (2004) [Pubmed]
  5. Manipulation of a nuclear NAD+ salvage pathway delays aging without altering steady-state NAD+ levels. Anderson, R.M., Bitterman, K.J., Wood, J.G., Medvedik, O., Cohen, H., Lin, S.S., Manchester, J.K., Gordon, J.I., Sinclair, D.A. J. Biol. Chem. (2002) [Pubmed]
  6. Telomeric and rDNA silencing in Saccharomyces cerevisiae are dependent on a nuclear NAD(+) salvage pathway. Sandmeier, J.J., Celic, I., Boeke, J.D., Smith, J.S. Genetics (2002) [Pubmed]
  7. Chemical activation of Sir2-dependent silencing by relief of nicotinamide inhibition. Sauve, A.A., Moir, R.D., Schramm, V.L., Willis, I.M. Mol. Cell (2005) [Pubmed]
  8. Two-dimensional protein map of an "ale"-brewing yeast strain: proteome dynamics during fermentation. Kobi, D., Zugmeyer, S., Potier, S., Jaquet-Gutfreund, L. FEMS Yeast Res. (2004) [Pubmed]
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