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Chemical Compound Review

Nickel 204     nickel(+2) cation

Synonyms: Nickel 211, Nickel 212, Nickel 213, Nickel 222, Nickel 223, ...
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Disease relevance of Nickel II ion


High impact information on Nickel II ion

  • We propose a general model for Ni(2+) recognition in which betaHis81 and two amino acids from the NH(2)-terminal part of the MHC bound peptide coordinate Ni(2+) which then interacts with some portion of the Valpha CDR1 or CDR2 region [6].
  • A series of experiments established that the functional ligand for this T cell was a preformed complex of Ni(2+) bound to the combination of DR52c and a specific peptide that was generated in human and mouse B cells, but not in fibroblasts nor other antigen processing-deficient cells [6].
  • The major histocompatibility complex (MHC) restriction element for a human Ni(2+) reactive T cell, ANi-2.3, was identified as DR52c [6].
  • The effects of carcinogenic nickel [(Ni) CAS: 7440-02-0] and Ni compounds on the natural killer (NK) cell activity of rat peripheral blood mononuclear cells (PBMCs) were studied [7].
  • In addition, some recent studies on CAOs whose active-site Cu(2+) has been replaced with Co(2+) or Ni(2+) are summarized [8].

Chemical compound and disease context of Nickel II ion

  • Reconstitution of metal-free recombinant Escherichia coli type I IDI with several divalent metals-Mg(2+), Mn(2+), Zn(2+), Co(2+), Ni(2+), and Cd(2+)-generated active enzyme [9].
  • Using Escherichia coli thioredoxin as a model protein, we show that potential binding sites can be identified using the Cu(2+) ion, and that pseudocontact shifts induced by a Ni(2+) ion bound to one of these sites can provide valuable long-range structure information about the protein [10].
  • Escherichia coli glyoxalase I (GlxI) is a metalloisomerase that is maximally activated by Ni(2+), unlike other known GlxI enzymes which are active with Zn(2+) [11].
  • The TG-induced sustained elevation of [Ca(2+)](i) in endothelial cells was inhibited completely by 1 mM Ni(2+) and partly by 10 microM econazole and 30 microM ML-9, but not by 900 ng ml(-1) pertussis toxin or 100 microM wortmannin [12].
  • In the present study, the hypoxia-mimicking agent Ni(2+) induced vasoactive intestinal peptide (VIP) expression at both mRNA and peptide levels but it did not modify the expression of VIP receptors (VPAC(1), VPAC(2) and PAC(1) receptors) in androgen-dependent human LNCaP prostate cancer cells [13].

Biological context of Nickel II ion

  • The urease activation kinetics were studied in vivo by monitoring the development of urease activity upon adding Ni(2+) to spectinomycin-treated Escherichia coli cells that expressed the complete K. aerogenes urease gene cluster with altered forms of ureE [14].
  • In addition, divalent cations (Mg(2+), Cu(2+), and Ni(2+)) have a dramatic and differential effect on the structure, depending on the state of phosphorylation of the protein [15].
  • For example, neither Ni(2+) nor the receptor-associated protein, which blocks binding of all known ligands to LRP, block alpha(2)M*-induced signal transduction [16].
  • In contrast, at basal membrane potentials (approximately 25 mV) ryanodine triggered extracellular Ca(2+) influx that was blocked by Ni(2+) [17].
  • Nickel ions localized initially in the cytoplasm, but later entered the nucleus and eventually silenced a transgene [18].

Anatomical context of Nickel II ion

  • Whereas rat myocytes lack I(Ca(T)) and exhibit I(Ca(TTX)) only, guinea pig myocytes possess both of these low-voltage-activated Ca(2+) currents, which are separated pharmacologically by superfusion with TTX or Ni(2+) [19].
  • Pretreatment of the oocytes with the reagent reduced Ni(2+) inhibition of the remaining current [20].
  • The precursor was purified from insoluble inclusion bodies by Ni(2+) affinity and gel filtration chromatography under denaturing conditions [21].
  • To study the ion selectivity of the AtNHX1 protein, we have purified a histidine-tagged version of the protein from yeast microsomes by Ni(2+) affinity chromatography, reconstituted the protein into lipid vesicles, and measured cation-dependent H(+) exchange with the fluorescent pH indicator pyranine [22].
  • Astrocyte death could be blocked by 100 microM Ni(2+) or 100 microM benzamil, suggesting involvement of Na(+)-Ca(2+) exchange [23].

Associations of Nickel II ion with other chemical compounds

  • At pH 8.5, binding of Zn(2+), Cd(2+), or Ni(2+) reduced the rates of proton uptake upon Q(A)(-) and Q(B)(-) formation as well as k(AB)((1)) by approximately an order of magnitude, resulting in similar final values, indicating that there is a common rate-limiting step [24].
  • Recent structural studies revealed that the active site of colicin DNases encompasses the HNH motif found in homing endonucleases, and bound within this motif a single transition metal ion (either Zn(2+) or Ni(2+)) the role of which is unknown [25].
  • Native UreE possesses a histidine-rich region at its carboxyl terminus that binds several equivalents of Ni(2+); however, a truncated form of this protein (H144*UreE) binds only 2 Ni(2+) per dimer and is functionally active (Brayman, T. G., and Hausinger, R. P. (1996) J. Bacteriol. 178, 5410-5416) [14].
  • In this study, we have employed a variety of spectroscopic and computational methods to probe the electronic structure of the NiSOD active site in both its oxidized (NiSOD(ox), possessing a low-spin (S = (1)/(2)) Ni(3+) center) and reduced (NiSOD(red), containing a diamagnetic Ni(2+) center) states [26].
  • The crystal structure, as determined by synchrotron X-ray powder diffraction, is a heavily distorted double perovskite with Ni(2+) and Mn(4+) ions ordered in a rock-salt configuration [27].

Gene context of Nickel II ion

  • CAX4 transcripts appeared to be expressed at low levels in all tissues and levels of CAX4 RNA increased after Mn(2+), Na(+), and Ni(2+) treatment [28].
  • The removal of extracellular Ca(2+) or stimulation in the presence of Ni(2+) (2mM) or La(3+) (100 microM) blocked the RANTES-elicited [Ca(2+)](i) increase [29].
  • Either VIP or hypoxia mimetics with Ni(2+) increased VEGF expression whereas both conditions together resulted in an additive response [13].
  • The C-terminally poly-his tagged Glc7p with and without an N-terminal hemagglutinin (HA) tag was partially purified by immobilized Ni(2+) affinity chromatography and further analyzed by gel filtration and ion exchange chromatography [30].
  • Neither RAP nor Ni(2+) blocked the binding of (125)I-alpha(2)M* to alpha(2)MSR on insulin- or buffer-treated cells, but they both blocked binding to LRP/alpha(2)MR [31].

Analytical, diagnostic and therapeutic context of Nickel II ion

  • A functional Ndh subcomplex was purified by Ni(2+) affinity chromatography and its subunit composition analyzed by mass spectrometry [32].
  • In fact, HSA could be replaced by xenogeneic albumins exhibiting sufficient affinity for Ni(2+) as determined by surface plasmon resonance (Biacore technology) or atomic absorption spectroscopy [33].
  • Western blot revealed that YggB-His(6) oligomers are more stable in the presence of Ni(2+), providing evidence that Ni(2+) is coordinated between C termini from different subunits of the channel [34].
  • Previously, we reported that tolerance to nickel, induced by oral administration of Ni(2+) ions, can be adoptively transferred to naive mice with only 10(2) splenic T cells [35].
  • L-754,394 that had been adducted to P450 3A4 was hydrolyzed under the conditions used for SDS-PAGE, Ni(2+) affinity chromatography, and proteolytic digestion [36].


  1. Chrysanthemyl diphosphate synthase: isolation of the gene and characterization of the recombinant non-head-to-tail monoterpene synthase from Chrysanthemum cinerariaefolium. Rivera, S.B., Swedlund, B.D., King, G.J., Bell, R.N., Hussey, C.E., Shattuck-Eidens, D.M., Wrobel, W.M., Peiser, G.D., Poulter, C.D. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  2. Molecular characterization of Vibrio parahaemolyticus vSGLT: a model for sodium-coupled sugar cotransporters. Turk, E., Kim, O., le Coutre, J., Whitelegge, J.P., Eskandari, S., Lam, J.T., Kreman, M., Zampighi, G., Faull, K.F., Wright, E.M. J. Biol. Chem. (2000) [Pubmed]
  3. Block of tetrodotoxin-resistant Na+ channel pore by multivalent cations: gating modification and Na+ flow dependence. Kuo, C.C., Chen, W.Y., Yang, Y.C. J. Gen. Physiol. (2004) [Pubmed]
  4. Apolipoprotein E and mimetic peptide initiate a calcium-dependent signaling response in macrophages. Misra, U.K., Adlakha, C.L., Gawdi, G., McMillian, M.K., Pizzo, S.V., Laskowitz, D.T. J. Leukoc. Biol. (2001) [Pubmed]
  5. Involvement of histone hypoacetylation in Ni2+-induced bcl- 2 down-regulation and human hepatoma cell apoptosis. Kang, J., Zhang, D., Chen, J., Lin, C., Liu, Q. J. Biol. Inorg. Chem. (2004) [Pubmed]
  6. Components of the ligand for a Ni++ reactive human T cell clone. Lu, L., Vollmer, J., Moulon, C., Weltzien, H.U., Marrack, P., Kappler, J. J. Exp. Med. (2003) [Pubmed]
  7. Inhibition of rat natural killer cell function by carcinogenic nickel compounds: preventive action of manganese. Judde, J.G., Breillout, F., Clemenceau, C., Poupon, M.F., Jasmin, C. J. Natl. Cancer Inst. (1987) [Pubmed]
  8. Tyrosine-derived quinone cofactors. Mure, M. Acc. Chem. Res. (2004) [Pubmed]
  9. Escherichia coli type I isopentenyl diphosphate isomerase: structural and catalytic roles for divalent metals. Lee, S., Poulter, C.D. J. Am. Chem. Soc. (2006) [Pubmed]
  10. Metal-protein interactions: structure information from Ni(2+)-induced pseudocontact shifts in a native nonmetalloprotein. Jensen, M.R., Led, J.J. Biochemistry (2006) [Pubmed]
  11. An XAS investigation of product and inhibitor complexes of Ni-containing GlxI from Escherichia coli: mechanistic implications. Davidson, G., Clugston, S.L., Honek, J.F., Maroney, M.J. Biochemistry (2001) [Pubmed]
  12. Mechanisms of the thapsigargin-induced Ca(2+) entry in in situ endothelial cells of the porcine aortic valve and the endothelium-dependent relaxation in the porcine coronary artery. Kuroiwa-Matsumoto, M., Hirano, K., Ahmed, A., Kawasaki, J., Nishimura, J., Kanaide, H. Br. J. Pharmacol. (2000) [Pubmed]
  13. Hypoxia regulation of expression and angiogenic effects of vasoactive intestinal peptide (VIP) and VIP receptors in LNCaP prostate cancer cells. Collado, B., Sánchez-Chapado, M., Prieto, J.C., Carmena, M.J. Mol. Cell. Endocrinol. (2006) [Pubmed]
  14. In vivo and in vitro kinetics of metal transfer by the Klebsiella aerogenes urease nickel metallochaperone, UreE. Colpas, G.J., Hausinger, R.P. J. Biol. Chem. (2000) [Pubmed]
  15. Isolation and characterization of IIAChb, a soluble protein of the enzyme II complex required for the transport/phosphorylation of N, N'-diacetylchitobiose in Escherichia coli. Keyhani, N.O., Boudker, O., Roseman, S. J. Biol. Chem. (2000) [Pubmed]
  16. The role of Grp 78 in alpha 2-macroglobulin-induced signal transduction. Evidence from RNA interference that the low density lipoprotein receptor-related protein is associated with, but not necessary for, GRP 78-mediated signal transduction. Misra, U.K., Gonzalez-Gronow, M., Gawdi, G., Hart, J.P., Johnson, C.E., Pizzo, S.V. J. Biol. Chem. (2002) [Pubmed]
  17. Ca(2+) influx through the osteoclastic plasma membrane ryanodine receptor. Moonga, B.S., Li, S., Iqbal, J., Davidson, R., Shankar, V.S., Bevis, P.J., Inzerillo, A., Abe, E., Huang, C.L., Zaidi, M. Am. J. Physiol. Renal Physiol. (2002) [Pubmed]
  18. Alterations of histone modifications and transgene silencing by nickel chloride. Ke, Q., Davidson, T., Chen, H., Kluz, T., Costa, M. Carcinogenesis (2006) [Pubmed]
  19. T-Type and tetrodotoxin-sensitive Ca(2+) currents coexist in guinea pig ventricular myocytes and are both blocked by mibefradil. Heubach, J.F., Köhler, A., Wettwer, E., Ravens, U. Circ. Res. (2000) [Pubmed]
  20. External nickel inhibits epithelial sodium channel by binding to histidine residues within the extracellular domains of alpha and gamma subunits and reducing channel open probability. Sheng, S., Perry, C.J., Kleyman, T.R. J. Biol. Chem. (2002) [Pubmed]
  21. Purified NS2B/NS3 serine protease of dengue virus type 2 exhibits cofactor NS2B dependence for cleavage of substrates with dibasic amino acids in vitro. Yusof, R., Clum, S., Wetzel, M., Murthy, H.M., Padmanabhan, R. J. Biol. Chem. (2000) [Pubmed]
  22. The arabidopsis Na+/H+ exchanger AtNHX1 catalyzes low affinity Na+ and K+ transport in reconstituted liposomes. Venema, K., Quintero, F.J., Pardo, J.M., Donaire, J.P. J. Biol. Chem. (2002) [Pubmed]
  23. Calcium dependence of rapid astrocyte death induced by transient hypoxia, acidosis, and extracellular ion shifts. Bondarenko, A., Chesler, M. Glia (2001) [Pubmed]
  24. Identification of the proton pathway in bacterial reaction centers: both protons associated with reduction of QB to QBH2 share a common entry point. Adelroth, P., Paddock, M.L., Sagle, L.B., Feher, G., Okamura, M.Y. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  25. Homing in on the role of transition metals in the HNH motif of colicin endonucleases. Pommer, A.J., Kühlmann, U.C., Cooper, A., Hemmings, A.M., Moore, G.R., James, R., Kleanthous, C. J. Biol. Chem. (1999) [Pubmed]
  26. Spectroscopic and computational studies of Ni superoxide dismutase: electronic structure contributions to enzymatic function. Fiedler, A.T., Bryngelson, P.A., Maroney, M.J., Brunold, T.C. J. Am. Chem. Soc. (2005) [Pubmed]
  27. Designed ferromagnetic, ferroelectric Bi(2)NiMnO(6). Azuma, M., Takata, K., Saito, T., Ishiwata, S., Shimakawa, Y., Takano, M. J. Am. Chem. Soc. (2005) [Pubmed]
  28. Characterization of CAX4, an Arabidopsis H(+)/cation antiporter. Cheng, N.H., Pittman, J.K., Shigaki, T., Hirschi, K.D. Plant Physiol. (2002) [Pubmed]
  29. Beta-chemokines and human immunodeficiency virus type-1 proteins evoke intracellular calcium increases in human microglia. Hegg, C.C., Hu, S., Peterson, P.K., Thayer, S.A. Neuroscience (2000) [Pubmed]
  30. Sds22p is a subunit of a stable isolatable form of protein phosphatase 1 (Glc7p) from Saccharomyces cerevisiae. Hong, G., Trumbly, R.J., Reimann, E.M., Schlender, K.K. Arch. Biochem. Biophys. (2000) [Pubmed]
  31. Coordinate regulation of the alpha(2)-macroglobulin signaling receptor and the low density lipoprotein receptor-related protein/alpha(2)-macroglobulin receptor by insulin. Misra, U.K., Gawdi, G., Gonzalez-Gronow, M., Pizzo, S.V. J. Biol. Chem. (1999) [Pubmed]
  32. New subunits NDH-M, -N, and -O, encoded by nuclear genes, are essential for plastid Ndh complex functioning in higher plants. Rumeau, D., Bécuwe-Linka, N., Beyly, A., Louwagie, M., Garin, J., Peltier, G. Plant Cell (2005) [Pubmed]
  33. Metal-protein complex-mediated transport and delivery of Ni2+ to TCR/MHC contact sites in nickel-specific human T cell activation. Thierse, H.J., Moulon, C., Allespach, Y., Zimmermann, B., Doetze, A., Kuppig, S., Wild, D., Herberg, F., Weltzien, H.U. J. Immunol. (2004) [Pubmed]
  34. C termini of the Escherichia coli mechanosensitive ion channel (MscS) move apart upon the channel opening. Koprowski, P., Kubalski, A. J. Biol. Chem. (2003) [Pubmed]
  35. Infectious nickel tolerance: a reciprocal interplay of tolerogenic APCs and T suppressor cells that is driven by immunization. Roelofs-Haarhuis, K., Wu, X., Nowak, M., Fang, M., Artik, S., Gleichmann, E. J. Immunol. (2003) [Pubmed]
  36. Mechanism-based inactivation of cytochrome P450 3A4 by L-754,394. Lightning, L.K., Jones, J.P., Friedberg, T., Pritchard, M.P., Shou, M., Rushmore, T.H., Trager, W.F. Biochemistry (2000) [Pubmed]
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